Artículo

Bouchet, A.M.; Frías, M.A.; Lairion, F.; Martini, F.; Almaleck, H.; Gordillo, G.; Disalvo, E.A. "Structural and dynamical surface properties of phosphatidylethanolamine containing membranes" (2009) Biochimica et Biophysica Acta - Biomembranes. 1788(5):918-925
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Abstract:

The hydration of solid dimyristoylphosphatidylethanolamine (DMPE) produces a negligible shift in the asymmetric stretching frequency of the phosphate groups in contrast to dimyristoylphosphatidylcholine (DMPC). This suggests that the hydration of DMPE is not a consequence of the disruption of the solid lattice of the phosphate groups as occurs in DMPC. The strong lateral interactions between NH3 and PO2 - groups present in the solid PEs remain when the lipids are fully hydrated and seem to be a limiting factor for the hydration of the phosphate group hindering the reorientation of the polar heads. The lower mobility is reflected in a higher energy to translocate the phosphoethanolamine (P-N) dipoles in an electrical field. This energy is decreased in the presence of increasing ratios of PCs of saturated chains in phosphoethanolamine monolayer. The association of PC and PE in the membrane affecting the reorientation of the P-N groups is dependent of the chain-chain interaction. The dipole potentials of PCs and PEs mixtures show different behaviors according to the saturation of the acyl chain. This was correlated with the area in monolayers and the hydration of the P-N groups. In spite of the low hydration, DMPE is still able to adsorb fully hydrated proteins, although in a lower rate than DMPC at the same surface pressure. This indicates that PE interfaces posses an excess of surface free energy to drive protein interaction. The relation of this free energy with the low water content is discussed. © 2009 Elsevier B.V. All rights reserved.

Registro:

Documento: Artículo
Título:Structural and dynamical surface properties of phosphatidylethanolamine containing membranes
Autor:Bouchet, A.M.; Frías, M.A.; Lairion, F.; Martini, F.; Almaleck, H.; Gordillo, G.; Disalvo, E.A.
Filiación:Laboratorio de Fisicoquimica de Membranas Lipidicas, Catedra de Quimica General e Inorgánica, Departamento de Química Analítica y Fisicoquímica, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, (1113), Buenos Aires, Argentina
Departamento de Química Inorgánica, Analítica y Química Física, Facultad de Ciencias Exactas y Naturales, INQUIMAE, Ciudad Universitaria, Pabellon 2, 1428 Buenos Aires, Argentina
Palabras clave:Cyclic voltammetry; Dipole potential; Interphase water activity; Monolayer; Phosphate asymmetric frequency; Phosphatidylethanolamine; Protein penetration; ammonia; dimyristoylphosphatidylcholine; dimyristoylphosphatidylethanolamine; ethanolamine derivative; lipid; long chain fatty acid coenzyme A ligase; nitrogen; phosphate; phosphatidylcholine; phosphatidylethanolamine; phosphorus; protein; unclassified drug; article; cell disruption; cell energy; controlled study; correlation analysis; dipole; electric field; energy absorption; gene translocation; hydration; interface pressure; interphase; membrane biology; membrane structure; molecular dynamics; molecular interaction; monolayer culture; polarization; priority journal; surface property; water content; Adsorption; Animals; Biophysical Phenomena; Lecithins; Membrane Lipids; Membrane Potentials; Membranes, Artificial; Micelles; Molecular Structure; Phosphatidylethanolamines; Pressure; Proteins; Spectroscopy, Fourier Transform Infrared; Surface Properties; Thermodynamics
Año:2009
Volumen:1788
Número:5
Página de inicio:918
Página de fin:925
DOI: http://dx.doi.org/10.1016/j.bbamem.2009.02.012
Título revista:Biochimica et Biophysica Acta - Biomembranes
Título revista abreviado:Biochim. Biophys. Acta Biomembr.
ISSN:00052736
CODEN:BBBMB
CAS:ammonia, 14798-03-9, 51847-23-5, 7664-41-7; dimyristoylphosphatidylcholine, 13699-48-4, 18194-24-6; dimyristoylphosphatidylethanolamine, 20255-95-2; lipid, 66455-18-3; long chain fatty acid coenzyme A ligase, 9013-18-7; nitrogen, 7727-37-9; phosphate, 14066-19-4, 14265-44-2; phosphatidylcholine, 55128-59-1, 8002-43-5; phosphatidylethanolamine, 1405-71-6; phosphorus, 7723-14-0; protein, 67254-75-5; 1,2-dimyristoylphosphatidylethanolamine, 20255-95-2; Lecithins; Membrane Lipids; Membranes, Artificial; Micelles; Phosphatidylethanolamines; Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00052736_v1788_n5_p918_Bouchet

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Citas:

---------- APA ----------
Bouchet, A.M., Frías, M.A., Lairion, F., Martini, F., Almaleck, H., Gordillo, G. & Disalvo, E.A. (2009) . Structural and dynamical surface properties of phosphatidylethanolamine containing membranes. Biochimica et Biophysica Acta - Biomembranes, 1788(5), 918-925.
http://dx.doi.org/10.1016/j.bbamem.2009.02.012
---------- CHICAGO ----------
Bouchet, A.M., Frías, M.A., Lairion, F., Martini, F., Almaleck, H., Gordillo, G., et al. "Structural and dynamical surface properties of phosphatidylethanolamine containing membranes" . Biochimica et Biophysica Acta - Biomembranes 1788, no. 5 (2009) : 918-925.
http://dx.doi.org/10.1016/j.bbamem.2009.02.012
---------- MLA ----------
Bouchet, A.M., Frías, M.A., Lairion, F., Martini, F., Almaleck, H., Gordillo, G., et al. "Structural and dynamical surface properties of phosphatidylethanolamine containing membranes" . Biochimica et Biophysica Acta - Biomembranes, vol. 1788, no. 5, 2009, pp. 918-925.
http://dx.doi.org/10.1016/j.bbamem.2009.02.012
---------- VANCOUVER ----------
Bouchet, A.M., Frías, M.A., Lairion, F., Martini, F., Almaleck, H., Gordillo, G., et al. Structural and dynamical surface properties of phosphatidylethanolamine containing membranes. Biochim. Biophys. Acta Biomembr. 2009;1788(5):918-925.
http://dx.doi.org/10.1016/j.bbamem.2009.02.012