Artículo

Luzzani, C.; Solari, C.; Losino, N.; Ariel, W.; Romorini, L.; Bluguermann, C.; Sevlever, G.; Barañao, L.; Miriuka, S.; Guberman, A. "Modulation of chromatin modifying factors' gene expression in embryonic and induced pluripotent stem cells" (2011) Biochemical and Biophysical Research Communications. 410(4):816-822
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Abstract:

Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) are a promising source of cells for regenerative medicine because of their potential of self renew and differentiation. Multiple evidences highlight the relationship of chromatin remodeling with stem cell properties, differentiation programs and reprogramming for iPSC obtention.With the purpose of finding chromatin modifying factors relevant to these processes, and based on ChIP on chip studies, we selected several genes that could be modulated by Oct4, Sox2 and Nanog, critical transcription factors in stem cells, and studied their expression profile along the differentiation in mouse and human ESCs, and in mouse iPSCs. In this work, we analyzed the expression of Gcn5l2, GTF3C3, TAF15, ATF7IP, Myst2, HDAC2, HDAC3, HDAC5, HDAC10, SUV39H2, Jarid2, and Bmi-1. We found some genes from different functional groups that were highly modulated, suggesting that they could be relevant both in the undifferentiated state and during differentiation. These findings could contribute to the comprehension of molecular mechanisms involved in pluripotency, early differentiation and reprogramming. We believe that a deeper knowledge of the epigenetic regulation of ESC will allow improving somatic cell reprogramming for iPSC obtention and differentiation protocols optimization. © 2011 Elsevier Inc.

Registro:

Documento: Artículo
Título:Modulation of chromatin modifying factors' gene expression in embryonic and induced pluripotent stem cells
Autor:Luzzani, C.; Solari, C.; Losino, N.; Ariel, W.; Romorini, L.; Bluguermann, C.; Sevlever, G.; Barañao, L.; Miriuka, S.; Guberman, A.
Filiación:Lab. de Regulacion de la Expresion Genica en el Crecimiento, Supervivencia y Diferenciacion Celular, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Buenos Aires, Argentina
Laboratorio de Biología del Desarrollo Celular, Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia (FLENI), Argentina
Departamento de Fisiología y Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Chromatin modifying factors; Differentiation; Embryonic stem cells; Induced pluripotent stem cells; BMI1 protein; cell protein; histone deacetylase; histone deacetylase 10; histone deacetylase 2; histone deacetylase 3; histone deacetylase 5; protein atf7ip; protein gcn512; protein gtf3c3; protein Jarid2; protein myst2; protein suv39h2; protein taf15; unclassified drug; animal cell; article; cell differentiation; chromatin assembly and disassembly; embryonic stem cell; gene expression; human; human cell; mouse; nonhuman; pluripotent stem cell; priority journal; protein expression; Animals; Cell Line; Chromatin; Chromatin Immunoprecipitation; Embryonic Stem Cells; Gene Expression Regulation; Humans; Induced Pluripotent Stem Cells; Mice; Transcription Factors
Año:2011
Volumen:410
Número:4
Página de inicio:816
Página de fin:822
DOI: http://dx.doi.org/10.1016/j.bbrc.2011.06.070
Título revista:Biochemical and Biophysical Research Communications
Título revista abreviado:Biochem. Biophys. Res. Commun.
ISSN:0006291X
CODEN:BBRCA
CAS:histone deacetylase, 9076-57-7; Chromatin; Transcription Factors
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v410_n4_p816_Luzzani

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Citas:

---------- APA ----------
Luzzani, C., Solari, C., Losino, N., Ariel, W., Romorini, L., Bluguermann, C., Sevlever, G.,..., Guberman, A. (2011) . Modulation of chromatin modifying factors' gene expression in embryonic and induced pluripotent stem cells. Biochemical and Biophysical Research Communications, 410(4), 816-822.
http://dx.doi.org/10.1016/j.bbrc.2011.06.070
---------- CHICAGO ----------
Luzzani, C., Solari, C., Losino, N., Ariel, W., Romorini, L., Bluguermann, C., et al. "Modulation of chromatin modifying factors' gene expression in embryonic and induced pluripotent stem cells" . Biochemical and Biophysical Research Communications 410, no. 4 (2011) : 816-822.
http://dx.doi.org/10.1016/j.bbrc.2011.06.070
---------- MLA ----------
Luzzani, C., Solari, C., Losino, N., Ariel, W., Romorini, L., Bluguermann, C., et al. "Modulation of chromatin modifying factors' gene expression in embryonic and induced pluripotent stem cells" . Biochemical and Biophysical Research Communications, vol. 410, no. 4, 2011, pp. 816-822.
http://dx.doi.org/10.1016/j.bbrc.2011.06.070
---------- VANCOUVER ----------
Luzzani, C., Solari, C., Losino, N., Ariel, W., Romorini, L., Bluguermann, C., et al. Modulation of chromatin modifying factors' gene expression in embryonic and induced pluripotent stem cells. Biochem. Biophys. Res. Commun. 2011;410(4):816-822.
http://dx.doi.org/10.1016/j.bbrc.2011.06.070