Abstract:
Previous results suggested that the number of androgen-binding sites in rat epididymis was controlled by androgens. Using an exchange technique for receptor determination, we now report that cytoplasmic receptor number decreased to about half the control value after 4 days of castration and reached its lowest level (30% of the control value) after 12 days of castration. The Kd for [3H]methyltrienolone for cytoplasmic receptor varied from 2.8 × 10−9 M in controls to 0.6 × 10−9 M (P < 0.001) after 6 or more days of castration. Nuclear binding sites were reduced to 17% of the control value from the second day of castration on, but no change in the affinity for methyltrienolone was noted. The administration of testosterone propionate (400 μg/day) to rats castrated for 20 days significantly increased the number of nuclear and cytoplasmic binding sites from the second and third days of treatment, respectively. Labeled thymidine incorporation into DNA rose significantly after a 4-day latency period. The proportion of total binding sites capable of translocation into the nucleus was elevated during the early phase (2–4 days) of androgen treatment. These results also suggest the heterogeneity of the cytoplasmic binding site population. Control epididymides were composed of 61.5% receptor-containing epithelial cells, and this proportion was significantly reduced to 53.2% after 20 days of castration. Androgen administration elevated this percentage after an 8-day latency period. Proteolytic activity in cytosol was increased over control values after castration (for 20 days) and until the fourth day after the onset of androgen treatment. However, this activity does not seem to be an important factor in the decrease in binding sites caused by orchidectomy. © 1983 by The Endocrine Society.
Registro:
Documento: |
Artículo
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Título: | Androgens control androgen-binding sites in rat epididymis |
Autor: | Tezon, J.G.; Blaquier, J.A. |
Filiación: | Institute de Biologia y Medicina Experimental, Laboratorio de Esteroides, Obligado 2490, Buenos Aires, 1428, Argentina
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Palabras clave: | androgen; cell nucleus receptor; cytosol receptor; radioisotope; testosterone propionate; animal cell; castration; drug efficacy; drug receptor binding; endocrine system; epididymis; male genital system; methyltrienolone h 3; nonhuman; pharmacokinetics; rat; thymidine h 3; Androgens; Animal; Castration; Cell Nucleus; Cytoplasm; Epididymis; Estrenes; Male; Metribolone; Peptide Hydrolases; Rats; Rats, Inbred Strains; Receptors, Androgen; Receptors, Steroid; Support, Non-U.S. Gov't; Testosterone |
Año: | 1983
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Volumen: | 113
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Número: | 3
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Página de inicio: | 1025
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Página de fin: | 1030
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DOI: |
http://dx.doi.org/10.1210/endo-113-3-1025 |
Título revista: | Endocrinology
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Título revista abreviado: | Endocrinology
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ISSN: | 00137227
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CAS: | testosterone propionate, 57-85-2; Androgens; Estrenes; Metribolone, 965-93-5; Peptide Hydrolases, EC 3.4; Receptors, Androgen; Receptors, Steroid; Testosterone, 57-85-2
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v113_n3_p1025_Tezon |
Referencias:
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- Calandra, R.S., Blaquier, J.A., Del Castillo, E.J., Rivarola, M.A., Androgen dependency of the androgen receptor in rat epididymis (1975) Biochem Biophys Res Commun, 67, p. 97
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- Calandra, R.S., Purvis, K., Attramadal, A., Hansson, V., Androgen receptors in rat epididymis do not disappear after castration (1977) J Steroid Biochem, 8, p. 1205
- Tezon, J.G., Vazquez, M.H., Blaquier, J.A., Androgen determined subcellular distribution of receptor in rat epididymis. 5a-DHT induced nuclear translocation is blocked by antiandrogens (1982) Endocrinology, 111, p. 2039
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Citas:
---------- APA ----------
Tezon, J.G. & Blaquier, J.A.
(1983)
. Androgens control androgen-binding sites in rat epididymis. Endocrinology, 113(3), 1025-1030.
http://dx.doi.org/10.1210/endo-113-3-1025---------- CHICAGO ----------
Tezon, J.G., Blaquier, J.A.
"Androgens control androgen-binding sites in rat epididymis"
. Endocrinology 113, no. 3
(1983) : 1025-1030.
http://dx.doi.org/10.1210/endo-113-3-1025---------- MLA ----------
Tezon, J.G., Blaquier, J.A.
"Androgens control androgen-binding sites in rat epididymis"
. Endocrinology, vol. 113, no. 3, 1983, pp. 1025-1030.
http://dx.doi.org/10.1210/endo-113-3-1025---------- VANCOUVER ----------
Tezon, J.G., Blaquier, J.A. Androgens control androgen-binding sites in rat epididymis. Endocrinology. 1983;113(3):1025-1030.
http://dx.doi.org/10.1210/endo-113-3-1025