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Abstract:

Use of molecular markers such as Random Amplified Polymorphic DNA (RAPD), Amplified Fragment Length Polymorphism (AFLP) and Simple Sequence Repeats (SSR) as descriptors to characterize and differentiate a set of 100 soybean varieties of commercial use in Argentina was taken as a leading case study for plant variety protection (PVP) purposes. Sixteen morphological traits were recorded to compare pedigree relationships among varieties with information derived from conventional descriptors and molecular markers. Analysis of 109 polymorphic loci confirmed the rather low genetic variability of commercial soybean germplasm. Still, genetic fingerprinting of the 100 varieties could be established. Calculated similarity indexes were dependent on the technique, ranging from 0.262 (SSR), 0.407 (RAPD), 0.400 (AFLP) and 0.574 (morphological traits). Dendrograms generated from morphological data matrix showed low value correlation with kinship coefficient matrix (r = 0.216). Still, they were suitable to identify and differentiate each of the 100 varieties analyzed; which was not possible with RAPD or AFLP markers using comparable numbers of polymorphic loci. SSR data showed the best fit to pedigree information (r = 0.353), while maintaining an association to morphologically based separation. Results suggest that the four techniques describe genetic variability in different and specific ways. A combination of SSR and morphological descriptors show the best compromise of regarding genetic relationships and the needs of clear classification for PVP and may help to establish minimum genetic distances for distinctness within PVP Office definition.

Registro:

Documento: Artículo
Título:Feasibility of integration of molecular markers and morphological descriptors in a real case study of a plant variety protection system for soybean
Autor:Giancola, S.; Marcucci Poltri, S.; Lacaze, P.; Hopp, H.E.
Filiación:Laboratorio de Marcadores Moleculares INASE, Av. Paseo Colon 922, Buenos Aires (1405), Argentina
Instituto de Biotecnologia CICVyA-CNIA, INTA Castelar, Argentina
AGROGENE, 620 Av. Blaise Pascal, Z.I, Moissy Cramayel CEDEX 77555, France
Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:AFLP; Glycine max (L.) Merr.; RAPD; SSR; UPGMA; Embryophyta; Glycine max
Año:2002
Volumen:127
Número:1
Página de inicio:95
Página de fin:113
DOI: http://dx.doi.org/10.1023/A:1019923923805
Título revista:Euphytica
Título revista abreviado:Euphytica
ISSN:00142336
CODEN:EUPHA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142336_v127_n1_p95_Giancola

Referencias:

  • Ajmone-Marsan, P., Castiglioni, P., Fusari, F., Kuiper, M., Motto, M., Genetic diversity and its relationship to hybrid performance in maize as revealed by RFLP and AFLP markers (1998) Theor Appl Genet, 98, pp. 219-227
  • Akkaya, M.S., Shoemaker, R.C., Specht, J.E., Bhagwat, A.A., Cregan, P.B., Integration of simple sequence repeats (SSR) DNA markers into a soybean linkage map (1995) Crop Sci, 35, pp. 1439-1445
  • Anderson, J.A., Churchill, G.A., Autrique, J.E., Tanksley, S.D., Sorrells, M.E., Optimizing parental selection for genetic linkage maps (1993) Genome, 36, pp. 181-186
  • Barret, B.A., Kidwell, K.K., AFLP-based genetic diversity assessment among wheat cultivars from the Pacific Northwest (1998) Crop Sci, 38, pp. 1261-1271
  • Becker, J., Vos, P., Kuiper, M., Salamini, F., Heun, M., Combined mapping of AFLP and RLPS markers in barley (1995) Mol Gen Genet, 249, pp. 65-73
  • Brown, S.M., Hopkins, M.S., Mitchell, S.E., Senior, M.L., Wang, T.Y., Duncan, R.R., Gonzalez-Candelas, F., Kresovich, S., Multiple methods for the identification of polymorphic simple sequence repeat (SSRs) in sorghum [Sorghum bicolor (L.) Moench] (1996) Theor Appl Genet, 93, pp. 190-198
  • Cervera, M.T., Gusmao, J., Steenackers, M., Peleman, J., Storme, V., Vanden Broeck, A., Van Montagu, M., Boerjan, W., Identification of AFLP molecular markers for resistance against Melanospora larici-populina in Populus (1996) Theor Appl Genet, 93, pp. 733-737
  • Clifford, H.T., Stephenson, W., (1975) An Introduction to Numerical Classification, , Academic Press, New York. 229 pp
  • Cregan, P.B., (1999) SoyBase Home Page, , http://129.186.26.94/SSR.html
  • Crisci, J.V., López Armengol, M.F., (1983) lntroducción a la Teoría y Práctica de la Taxonomía Numérica, , Secretaría General de la Organización de los Estados Americanos. Programa Regional de Desarrollo Científico y Tecnológico, Washington, D.C., 132 pp
  • Diwan, N., Cregan, P.B., Automated sizing of fluorescentlabeled simple sequence repeats (SSR) markers to assay genetic variation in soybean (1997) Theor Appl Genet, 95, pp. 723-733
  • Domini, P., Law, J.R., Koebner, R.M.D., Reeves, J.C., Cooke, R.J., Temporal trends in the diversity of UK wheat (2000) Theor Appl Genet, 100, pp. 912-917
  • Dos Santos, J.B., Nienhuis, J., Skroch, P., Tivang, J.G., Slocum, M.K., Comparison of RAPD and RFLP genetic markers in determining genetic similarity among Brassica oleracea L. genotypes (1994) Theor Appl Genet, 87, pp. 909-915
  • Giorda, L., Baigorri, H., (1997) El Cultivo de la Soja en Argentina, , INTA. Centro Regional Córdoba. EEA Marcos Juárez-EEA Manfredi. Coordinación Subprograma Soja. 448 pp
  • González, H.R., (1999) National Seed Institute, Yearbook, , Estilos Gráficos S.A. Publisher, Buenos Aires-Argentina. 84 pp
  • Kein, P., Beavis, W., Schupp, J., Freestone, R., Evaluation of soybean RFLP marker diversity in adapted germplasm (1992) Theor Appl Genet, 85, pp. 205-212
  • Liu, J.-J., Kuo, J., Novel PCR-Based Assay for plant and Bacterial DNA Fingerprinting (1995) Focus, 17, pp. 66-70
  • Lodhi, M.A., Ye, G.-N., Weeden, N.F., Reisch, I., A simple and efficient method for DNA extraction from grapevine cultivars and vitis species (1994) Plant Mol Biol, 12, pp. 6-13
  • Manifesto, M.M., Schlatter, A.R., Hopp, H.E., Suarez, E.Y., Dubcovsky, J., Quantitative evaluation of genetic diversity in wheat germplasm using molecular markers (2001) Crop Sciences, 41. , in press
  • Mantel, N., The detection of disease clustering and a generalized regression approach (1967) Cancer Res, 27, pp. 209-220
  • Maughan, P.J., Saghai Maroof, M.A., Buss, G.R., Microsatellite and amplified sequence length polymorphisms in cultivated and wild soybean (1995) Genome, 38, pp. 715-723
  • Milbourne, D., Meyer, R., Bradshow, J.E., Baird, E., Bonar, N., Powel, J.W., Morgante, M., Rafalsky, J.A., Comparison of PCR-based marker systems for the analysis of genetic relationships in cultivated potato (1997) Mol Breed, 3, pp. 127-136
  • Powell, J.W., Morgante, M., Andre, C., Hanafey, M., Vogel, M., Tingey, S.V., Rafalski, A., The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis (1996) Mol Breed, 2, pp. 225-238
  • Rohlf, F.J., (1992) NTSYS-pc. Numerical Taxonomy and Multivariate Analysis System Version 1.7, , Exeter Publ.LTD Setauket, NY
  • Rongwen, J., Akkaya, M.S., Bhagwat, A.A., Lavi, U., Cregan, P.B., The use of microsatellite DNA markers for soybean genotype identification (1995) Theor Appl Genet, 90, pp. 43-48
  • Sambrook, J., Fritsch, E.F., Maniatis, T., (1989) Molecular Cloning. A Laboratory Manual. 2nd. Ed., , Cold Spring Harbor Laboratory Press, New York
  • Sharma, S.K., Knox, M.R., Ellis, T.H.M., AFLP analysis of the diversity and phylogeny of Lens and its comparison with RAPD analysis (1996) Theor Appl Genet, 93, pp. 751-758
  • Smith, S., Chin, E., (1992) Pioneer Hi-Bred International. Application of RAPD Technology to Plant Breeding. Joint Plant Breeding Symposia, pp. 46-49. , Minneapolis, Minnesota, EUA
  • Smith, S., Register III, Genetic purity and testing technologies for seed quality: A company perspective (1998) Seed Sci Res, 8, pp. 285-293
  • Smith, S., Registrer, J., (1998) Genetic Purity and Testing Technologies. DNA-based Methodologies Replace Biochemistry, , PROPHYTA, ISTA Congress OECD, Pretoria, South Africa. 5053 pp
  • Sneath, P.H.A., Sokal, R.R., (1973) Numerical Taxonomy, 573. , The Principles and Practice of Numerical Classification, Freeman, San Francisco, CA., XV, 573 pp
  • Tautz, D., Trick, M., Dover, G.A., Cryptic simplicity in DNA is a major source of genetic variation (1986) Nature, 322, pp. 652-656
  • Thormann, C.E., Ferreira, M.E., Camargo, L.E.A., Tivang, J.G., Osborn, T.C., Comparison of RFLP and RAPD markers to estimating genetic relationships within and among cruciferous species (1994) Theor Appl Genet, 88, pp. 973-980
  • Tinker, N.A., Mather, D.E., KIN: Software for computing kinship coefficients (1993) J Heredity, 84, p. 238
  • (1983) Guidelines for the Conduct of Test for Distinctness, Homogeneity and Stability, Soybean (Glycine max (L.) Merrill)
  • Vos, P., Hogers, R., Bleeker, M., Reijans, M., Van de Lee, T., Homes, M., Frijters, A., Zabeau, M., AFLP: A new technique for DNA fingerprinting (1995) Nucl Acids Res, 21, pp. 4414-4470
  • Welsh, J., McClelland, M., Fingerprinting genomes using PCR with arbitrary primers (1990) Nucl Acids Res, 18, pp. 7213-7218
  • Williams, J.G.K., Kubelik, A.R., Livak, K.J., Rafalsky, J.A., Tingey, S.V., DNA polimorphisms amplified by arbitrary primers are useful as genetic markers (1990) Nucl Acids Res, 18, pp. 6331-6336

Citas:

---------- APA ----------
Giancola, S., Marcucci Poltri, S., Lacaze, P. & Hopp, H.E. (2002) . Feasibility of integration of molecular markers and morphological descriptors in a real case study of a plant variety protection system for soybean. Euphytica, 127(1), 95-113.
http://dx.doi.org/10.1023/A:1019923923805
---------- CHICAGO ----------
Giancola, S., Marcucci Poltri, S., Lacaze, P., Hopp, H.E. "Feasibility of integration of molecular markers and morphological descriptors in a real case study of a plant variety protection system for soybean" . Euphytica 127, no. 1 (2002) : 95-113.
http://dx.doi.org/10.1023/A:1019923923805
---------- MLA ----------
Giancola, S., Marcucci Poltri, S., Lacaze, P., Hopp, H.E. "Feasibility of integration of molecular markers and morphological descriptors in a real case study of a plant variety protection system for soybean" . Euphytica, vol. 127, no. 1, 2002, pp. 95-113.
http://dx.doi.org/10.1023/A:1019923923805
---------- VANCOUVER ----------
Giancola, S., Marcucci Poltri, S., Lacaze, P., Hopp, H.E. Feasibility of integration of molecular markers and morphological descriptors in a real case study of a plant variety protection system for soybean. Euphytica. 2002;127(1):95-113.
http://dx.doi.org/10.1023/A:1019923923805