Navegar

Colección

Datos Estadísticas

Artículo

La versión final de este artículo es de uso interno. El editor solo permite incluir en el repositorio el artículo en su versión post-print. Por favor, si usted la posee enviela a
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

The E2 transcriptional activator of the human papillomavirus regulates the expression of most viral transcripts. Its binding to specific target DNA sequences involves large conformational changes in the interacting macromolecules. The high stability of the E2:DNA complex prompted us to analyze the role of macromolecular interactions and adjuvant emulsions in the appearance of conformation-specific antibodies. We demonstrate that immunization with free or DNA-complexed E2 emulsified in an oil-in-water adjuvant elicits a humoral response shifted to the recognition of discontinuous epitopes. Epitope mapping and functional analysis of the generated anti-E2 mAbs reveals that two separate antibodies populations can be obtained: those able to form a stable ternary complex with protein and DNA, and those which recognize the DNA-binding surface of the transcription factor, interfering with E2 binding to DNA. © 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

Registro:

Documento: Artículo
Título:Antibody response to a viral transcriptional regulator
Autor:Cerutti, M.L.; Centeno, J.M.; De Prat-Gay, G.; Goldbaum, F.A.
Filiación:F. I. L. IIBBA-CONICET, IIB-FCEN-UBA, Av. Patricias Argentinas 435, Buenos Aires 1405, Argentina
Palabras clave:Adjuvant; Antibody; Antigen binding; Conformational epitope; Transcription factor; adjuvant; epitope; monoclonal antibody; transcription factor; transcription factor E2; unclassified drug; antibody response; antibody specificity; article; DNA binding; DNA determination; DNA sequence; emulsion; enzyme regulation; enzyme stability; epitope mapping; humoral immunity; immunization; macromolecule; molecular recognition; nonhuman; Papilloma virus; priority journal; protein conformation; protein interaction; transcription initiation; transcription regulation; virus expression; virus transcription; Human papillomavirus; Papillomavirus
Año:2003
Volumen:534
Número:1-3
Página de inicio:202
Página de fin:206
DOI: http://dx.doi.org/10.1016/S0014-5793(02)03850-4
Título revista:FEBS Letters
Título revista abreviado:FEBS Lett.
ISSN:00145793
CODEN:FEBLA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00145793_v534_n1-3_p202_Cerutti

Referencias:

  • McBride, A., Romanczuk, H., Howley, P.M., (1991) J. Biol. Chem., 266, pp. 18411-18414
  • Lima, L.M., Prat Gay G. de, (1997) J. Biol. Chem., 272, pp. 19295-19303
  • Ferreiro, D.U., Lima, L.M.T.R., Alonso, L.G., Goldbaum, F.A., Prat Gay G. de, (2000) Biochemistry, 39, pp. 14692-14701
  • Moore, J.P., Trkola, A., Dragic, T., (1997) Curr. Opin. Immunol., 9, pp. 551-562
  • Cerutti, M.L., Centeno, J.M., Goldbaum, F.A., Prat Gay G. de, (2001) J. Biol. Chem., 276, pp. 12769-12773
  • Ulrich, J.T., Myers, K.R., (1995) Vaccine Design: The Subunit and Adjuvant Approach, 6, pp. 495-524. , Powell and Newman, Eds., Plenum Press, New York
  • Vogel, F.R., Powell, M.F., (1995) Vaccine Design: The Subunit and Adjuvant Approach, 6, pp. 141-228. , Powell and Newman, Eds., Plenum Press, New York
  • Galfré, G.A., Milstein, C., (1981) Methods Enzymol., 73, pp. 3-46
  • Goldbaum, F.A., Cauerhff, A., Velikovsky, C.A., Llera, A.S., Riottot, M., Poljak, R.J., (1999) J. Immunol., 162, pp. 6040-6045
  • Hassen, E., Chaieb, A., Bouaouina, N., Khairi, H., Remadi, S., Chouchane, L., (2001) Mod. Asp. Immunobiol., 2, pp. 8-12
  • Foguel, D., Silva, J.L., Prat Gay G. de, (1998) J. Biol. Chem., 273, pp. 9050-9057
  • Hegde, R.S., Androphy, E.J., (1998) J. Mol. Biol., 284, pp. 1479-1489
  • Scibienski, R.J., (1973) J. Immunol., 111, pp. 114-120
  • Kang, C.L., Hariharan, K., Nara, P.L., Sodroski, J., Moore, J.P., (1994) J. Virol., 68, pp. 5854-5862
  • Kurg, R., Parik, J., Juronen, E., Sedman, T., Abroi, A., Liiv, I., Langel, U., Ustav, M., (1999) J. Virol., 73, pp. 4670-4677
  • Orten, D.J., Strawhecker, J.M., Sanderson, S.D., Huang, D., Prystowsky, M.B., Hinrichs, S.H., (1994) J. Biol. Chem., 269, pp. 32254-32263
  • Kim, S.S., Tam, J.K., Wang, A.F., Hegde, R.S., (2000) J. Biol. Chem., 275, pp. 31245-31254
  • Gauthier, J.-M., Dillner, J., Yaniv, M., (1991) Nucleic Acids Res., 19, pp. 7073-7079
  • Thompson, N.E., Strasheim, L.A., Nolan, K.M., Burgess, R.R., (1995) J. Biol. Chem., 270, pp. 4735-4740
  • Marasco, W.A., (2001) Curr. Top. Microbiol. Immunol., 286, pp. 587-594

Citas:

---------- APA ----------
Cerutti, M.L., Centeno, J.M., De Prat-Gay, G. & Goldbaum, F.A. (2003) . Antibody response to a viral transcriptional regulator. FEBS Letters, 534(1-3), 202-206.
http://dx.doi.org/10.1016/S0014-5793(02)03850-4
---------- CHICAGO ----------
Cerutti, M.L., Centeno, J.M., De Prat-Gay, G., Goldbaum, F.A. "Antibody response to a viral transcriptional regulator" . FEBS Letters 534, no. 1-3 (2003) : 202-206.
http://dx.doi.org/10.1016/S0014-5793(02)03850-4
---------- MLA ----------
Cerutti, M.L., Centeno, J.M., De Prat-Gay, G., Goldbaum, F.A. "Antibody response to a viral transcriptional regulator" . FEBS Letters, vol. 534, no. 1-3, 2003, pp. 202-206.
http://dx.doi.org/10.1016/S0014-5793(02)03850-4
---------- VANCOUVER ----------
Cerutti, M.L., Centeno, J.M., De Prat-Gay, G., Goldbaum, F.A. Antibody response to a viral transcriptional regulator. FEBS Lett. 2003;534(1-3):202-206.
http://dx.doi.org/10.1016/S0014-5793(02)03850-4