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Abstract:

In view of the present controversy related to the potential beneficial effects of clinical dehydroepiandrosterone (DHEA) treatments, and considering our own previous results that reveal an influence of this steroid in pituitary hyperplasia development in vivo in rats, we decided to evaluate the role of DHEA in prolactin and GH secretion, as well as in second messengers involved, in cultured rat anterior pituitary cells. DHEA (1 × 10-5 to 1 × 10-7 M) did not modify basal GH or prolactin release, and a prolactin inhibitory effect was observed only for androstenediol, a metabolite of DHEA. DHEA partially prevented dopamine (1 × 10-6 M)-induced prolactin inhibition and facilitated the prolactin-releasing effect of 10-8 M Ang II, without modifying the resulting Ca2+i mobilization. Furthermore, DHEA potentiated the GH release and cAMP production induced by 1 × 10-8 M GHRH. Finally, DHEA partially reversed the inhibitory effect of 1 × 10-8 M somatostatin on GH, but not prolactin, release. We conclude that DHEA in vitro, directly or indirectly through conversion into metabolites, is able to modulate the hormonal response of the pituitary to hypothalamic regulators. It can enhance pituitary prolactin release and induce GH secretion. These effects could help explain some of the side effects observed in prolonged DHEA treatments in vivo and should be taken into account when considering its use in human clinical trials. © 2005 Society for Endocrinology.

Registro:

Documento: Artículo
Título:Dehydroepiandrosterone (DHEA) modulates GHRH, somatostatin and angiotensin II action at the pituitary level
Autor:Suárez, C.; Vela, J.; García-Tornadú, I.; Becu-Villalobos, D.
Filiación:Instituto de Biología y Medicina Experimental, CONICET, V Obligado 2490, Buenos Aires 1428, Argentina
Palabras clave:androstenediol; angiotensin; calcium ion; cyclic AMP; dopamine; drug metabolite; growth hormone; growth hormone releasing factor; prasterone; prolactin; somatostatin; adenohypophysis; animal cell; article; calcium mobilization; concentration response; controlled study; cytokine production; female; growth hormone release; hormonal regulation; hormone inhibition; hormone response; hypophysis cell; hypothalamus; in vitro study; nonhuman; priority journal; prolactin release; rat; Angiotensin II; Animals; Calcium; Cell Culture Techniques; Cyclic AMP; Dehydroepiandrosterone; Female; Glucocorticoids; Growth Hormone; Growth Hormone-Releasing Hormone; Pituitary Gland, Anterior; Prolactin; Rats; Rats, Sprague-Dawley; Somatostatin; Stimulation, Chemical
Año:2005
Volumen:185
Número:1
Página de inicio:165
Página de fin:172
DOI: http://dx.doi.org/10.1677/joe.1.05889
Título revista:Journal of Endocrinology
Título revista abreviado:J. Endocrinol.
ISSN:00220795
CODEN:JOENA
CAS:androstenediol, 28652-91-7, 521-17-5; angiotensin, 1407-47-2; calcium ion, 14127-61-8; cyclic AMP, 60-92-4; dopamine, 51-61-6, 62-31-7; growth hormone releasing factor, 83930-13-6, 9034-39-3; growth hormone, 36992-73-1, 37267-05-3, 66419-50-9, 9002-72-6; prasterone, 53-43-0; prolactin, 12585-34-1, 50647-00-2, 9002-62-4; somatostatin, 38916-34-6, 51110-01-1; Angiotensin II, 11128-99-7; Calcium, 7440-70-2; Cyclic AMP, 60-92-4; Dehydroepiandrosterone, 53-43-0; Glucocorticoids; Growth Hormone, 9002-72-6; Growth Hormone-Releasing Hormone, 9034-39-3; Prolactin, 9002-62-4; Somatostatin, 51110-01-1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220795_v185_n1_p165_Suarez

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Citas:

---------- APA ----------
Suárez, C., Vela, J., García-Tornadú, I. & Becu-Villalobos, D. (2005) . Dehydroepiandrosterone (DHEA) modulates GHRH, somatostatin and angiotensin II action at the pituitary level. Journal of Endocrinology, 185(1), 165-172.
http://dx.doi.org/10.1677/joe.1.05889
---------- CHICAGO ----------
Suárez, C., Vela, J., García-Tornadú, I., Becu-Villalobos, D. "Dehydroepiandrosterone (DHEA) modulates GHRH, somatostatin and angiotensin II action at the pituitary level" . Journal of Endocrinology 185, no. 1 (2005) : 165-172.
http://dx.doi.org/10.1677/joe.1.05889
---------- MLA ----------
Suárez, C., Vela, J., García-Tornadú, I., Becu-Villalobos, D. "Dehydroepiandrosterone (DHEA) modulates GHRH, somatostatin and angiotensin II action at the pituitary level" . Journal of Endocrinology, vol. 185, no. 1, 2005, pp. 165-172.
http://dx.doi.org/10.1677/joe.1.05889
---------- VANCOUVER ----------
Suárez, C., Vela, J., García-Tornadú, I., Becu-Villalobos, D. Dehydroepiandrosterone (DHEA) modulates GHRH, somatostatin and angiotensin II action at the pituitary level. J. Endocrinol. 2005;185(1):165-172.
http://dx.doi.org/10.1677/joe.1.05889