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Salamone, G.V.; Petracca, Y.; Bass, J.I.F.; Rumbo, M.; Nahmod, K.A.; Gabelloni, M.L.; Vermeulen, M.E.; Matteo, M.J.; Geffner, J.R.; Trevani, A.S. "Flagellin delays spontaneous human neutrophil apoptosis" (2010) Laboratory Investigation. 90(7):1049-1059
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Abstract:

Neutrophils are short-lived cells that rapidly undergo apoptosis. However, their survival can be regulated by signals from the environment. Flagellin, the primary component of the bacterial flagella, is known to induce neutrophil activation. In this study we examined the ability of flagellin to modulate neutrophil apoptosis. Neutrophils cultured for 12 and 24 h in the presence of flagellin from Salmonella thyphimurim at concentrations found in pathological situations underwent a marked prevention of apoptosis. In contrast, Helicobacter pylori flagellin did not affect neutrophil survival, suggesting that Salmonella flagellin exerts the antiapoptotic effect by interacting with TLR5. The delaying in apoptosis mediated by Salmonella flagellin was coupled to higher expression levels of the antiapoptotic protein Mcl-1 and lower levels of activated caspase-3. Analysis of the signaling pathways indicated that Salmonella flagellin induced the activation of the p38 and ERK1/2 MAPK pathways as well as the PI3K/Akt pathway. Furthermore, it also stimulated IBα degradation and the phosphorylation of the p65 subunit, suggesting that Salmonella flagellin also triggers NF-B activation. Moreover, the pharmacological inhibition of ERK1/2 pathway and NF-B activation partially prevented the antiapoptotic effects exerted by flagellin. Finally, the apoptotic delaying effect exerted by flagellin was also evidenced when neutrophils were cultured with whole heat-killed S. thyphimurim. Both a wild-type and an aflagellate mutant S. thyphimurim strain promoted neutrophil survival; however, when cultured in low bacteria/neutrophil ratios, the flagellate bacteria showed a higher capacity to inhibit neutrophil apoptosis, although both strains showed a similar ability to induce neutrophil activation. Taken together, our results indicate that flagellin delays neutrophil apoptosis by a mechanism partially dependent on the activation of ERK1/2 MAPK and NF-B. The ability of flagellin to delay neutrophil apoptosis could contribute to perpetuate the inflammation during infections with flagellated bacteria. © 2010 USCAP, Inc All rights reserved.

Registro:

Documento: Artículo
Título:Flagellin delays spontaneous human neutrophil apoptosis
Autor:Salamone, G.V.; Petracca, Y.; Bass, J.I.F.; Rumbo, M.; Nahmod, K.A.; Gabelloni, M.L.; Vermeulen, M.E.; Matteo, M.J.; Geffner, J.R.; Trevani, A.S.
Filiación:Departamento de Inmunología, Instituto de Investigaciones Hematológicas, Academia Nacional de Medicina, Buenos Aires, Argentina
Departamento de Microbiología, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina
Departamento de Fisiología y Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Laboratorio de Investigaciones en El Sistema Inmune, Facultad de Ciencias Exactas, UNLP, La Plata, Argentina
Palabras clave:apoptosis; flagellin; neutrophil; 2 (2 amino 3 methoxyphenyl)chromone; 3 (4 methylphenylsulfonyl) 2 propenenitrile; 4 (4 fluorophenyl) 2 (4 methylsulfinylphenyl) 5 (4 pyridyl)imidazole; caspase 3; flagellin; I kappa B alpha; interleukin 8; mitogen activated protein kinase 1; mitogen activated protein kinase 3; mitogen activated protein kinase p38; phosphatidylinositol 3 kinase; protein kinase B; protein mcl 1; toll like receptor 5; wortmannin; caspase 3; flagellin; immunoglobulin enhancer binding protein; myeloid cell leukemia sequence 1 protein; protein bcl 2; apoptosis; article; bacterium mutant; cell survival; controlled study; cytokine production; enzyme phosphorylation; flow cytometry; fluorescence microscopy; Helicobacter pylori; human; human cell; leukocyte activation; neutrophil; priority journal; protein degradation; protein expression; Salmonella typhimurium; signal transduction; wild type; cell culture; drug effect; enzymology; flagellum; immunology; metabolism; physiology; salmonellosis; Apoptosis; Caspase 3; Cell Survival; Cells, Cultured; Flagella; Flagellin; Humans; MAP Kinase Signaling System; Neutrophils; NF-kappa B; Proto-Oncogene Proteins c-bcl-2; Salmonella Infections; Salmonella typhimurium
Año:2010
Volumen:90
Número:7
Página de inicio:1049
Página de fin:1059
DOI: http://dx.doi.org/10.1038/labinvest.2010.77
Título revista:Laboratory Investigation
Título revista abreviado:Lab. Invest.
ISSN:00236837
CODEN:LAINA
CAS:2 (2 amino 3 methoxyphenyl)chromone, 167869-21-8; 4 (4 fluorophenyl) 2 (4 methylsulfinylphenyl) 5 (4 pyridyl)imidazole, 152121-47-6; I kappa B alpha, 151217-48-0; caspase 3, 169592-56-7; flagellin, 12777-81-0; interleukin 8, 114308-91-7; mitogen activated protein kinase 1, 137632-08-7; mitogen activated protein kinase 3, 137632-07-6; phosphatidylinositol 3 kinase, 115926-52-8; protein kinase B, 148640-14-6; wortmannin, 19545-26-7; protein bcl 2, 219306-68-0; Caspase 3, 3.4.22.-; Flagellin, 12777-81-0; NF-kappa B; Proto-Oncogene Proteins c-bcl-2; myeloid cell leukemia sequence 1 protein
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00236837_v90_n7_p1049_Salamone.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00236837_v90_n7_p1049_Salamone

Referencias:

  • Kantari, C., Pederzoli-Ribeil, M., Witko-Sarsat, V., The role of neutrophils and monocytes in innate immunity (2008) Contrib Microbiol, 15, pp. 118-146
  • Nathan, C., Neutrophils and immunity: Challenges and opportunities (2006) Nat Rev Immunol, 6, pp. 173-182
  • Maggini, J., Raiden, S., Salamone, G., Regulation of neutrophil apoptosis by cytokines, pathogens and environmental stressors (2009) Front Biosci, 14, pp. 2372-2385
  • Colotta, F., Re, F., Polentarutti, N., Modulation of granulocyte survival and programmed cell death by cytokines and bacterial products (1992) Blood, 80, pp. 2012-2020
  • Yamamoto, C., Yoshida, S., Taniguchi, H., Lipopolysaccharide and granulocyte colony-stimulating factor delay neutrophil apoptosis and ingestion by guinea pig macrophages (1993) Infect Immun, 61, pp. 1972-1979
  • Liles, W.C., Dale, D.C., Klebanoff, S.J., Glucocorticoids inhibit apoptosis of human neutrophils (1995) Blood, 86, pp. 3181-3188
  • Trevani, A.S., Andonegui, G., Giordano, M., Neutrophil apoptosis induced by proteolytic enzymes (1996) Lab Invest, 74, pp. 711-721
  • Watson, R.W., Redmond, H.P., Wang, J.H., Neutrophils undergo apoptosis following ingestion of Escherichia coli (1996) J Immunol, 156, pp. 3986-3992
  • Gamberale, R., Giordano, M., Trevani, A.S., Modulation of human neutrophil apoptosis by immune complexes (1998) J Immunol, 161, pp. 3666-3674
  • Steiner, T.S., How flagellin and Toll-like receptor 5 contribute to enteric infection (2007) Infect Immun, 75, pp. 545-552
  • Ramos, H.C., Rumbo, M., Sirard, J.C., Bacterial flagellins: Mediators of pathogenicity and host immune responses in mucosa (2004) Trends Microbiol, 12, pp. 509-517
  • Hayashi, F., Smith, K.D., Ozinsky, A., The innate immune response to bacterial flagellin is mediated by Toll-like receptor 5 (2001) Nature, 410, pp. 1099-1103
  • Smith, K.D., Ozinsky, A., Toll-like receptor-5 and the innate immune response to bacterial flagellin (2002) Curr Top Microbiol Immunol, 270, pp. 93-108
  • Miao, E.A., Andersen-Nissen, E., Warren, S.E., TLR5 and Ipaf: Dual sensors of bacterial flagellin in the innate immune system (2007) Semin Immunopathol, 29, pp. 275-288
  • McDermott, P.F., Ciacci-Woolwine, F., Snipes, J.A., High-affinity interaction between Gram-negative flagellin and a cell surface polypeptide results in human monocyte activation (2000) Infect Immun, 68, pp. 5525-5529
  • Gewirtz, A.T., Simon Jr., P.O., Schmitt, C.K., Salmonella typhimurium translocates flagellin across intestinal epithelia, inducing a proinflammatory response (2001) J Clin Invest, 107, pp. 99-109
  • Sierro, F., Dubois, B., Coste, A., Flagellin stimulation of intestinal epithelial cells triggers CCL20-mediated migration of dendritic cells (2001) Proc Natl Acad Sci USA, 98, pp. 13722-13727
  • Crellin, N.K., Garcia, R.V., Hadisfar, O., Human CD4+ T cells express TLR5 and its ligand flagellin enhances the suppressive capacity and expression of FOXP3 in CD4+CD25+ T regulatory cells (2005) J Immunol, 175, pp. 8051-8059
  • Hayashi, F., Means, T.K., Luster, A.D., Toll-like receptors stimulate human neutrophil function (2003) Blood, 102, pp. 2660-2669
  • Vijay-Kumar, M., Wu, H., Jones, R., Flagellin suppresses epithelial apoptosis and limits disease during enteric infection (2006) Am J Pathol, 169, pp. 1686-1700
  • Catalano, M., Matteo, M., Barbolla, R.E., Helicobacter pylori vacA genotypes, cagA status and ureA-B polymorphism in isolates recovered from an Argentine population (2001) Diagn Microbiol Infect Dis, 41, pp. 205-210
  • Boyum, A., Separation of leukocytes from blood and bone marrow. Introduction (1968) Scand J Clin Lab Invest Suppl, 97, p. 7
  • Salamone, G., Giordano, M., Trevani, A.S., Promotion of neutrophil apoptosis by TNF-alpha (2001) J Immunol, 166, pp. 3476-3483
  • Coxon, A., Tang, T., Mayadas, T.N., Cytokine-activated endothelial cells delay neutrophil apoptosis in vitro and in vivo. A role for granulocyte/macrophage colony-stimulating factor (1999) J Exp Med, 190, pp. 923-934
  • Homburg, C.H., De Haas, M., Von Dem Borne, A.E., Human neutrophils lose their surface Fc gamma RIII and acquire annexin v binding sites during apoptosis in vitro (1995) Blood, 85, pp. 532-540
  • Andersen-Nissen, E., Smith, K.D., Strobe, K.L., Evasion of Toll-like receptor 5 by flagellated bacteria (2005) Proc Natl Acad Sci USA, 102, pp. 9247-9252
  • Francois, S., El Benna, J., Dang, P.M., Inhibition of neutrophil apoptosis by TLR agonists in whole blood: Involvement of the phosphoinositide 3-kinase/Akt and NF-kappaB signaling pathways, leading to increased levels of Mcl-1, A1, and phosphorylated Bad (2005) J Immunol, 174, pp. 3633-3642
  • Klein, J.B., Rane, M.J., Scherzer, J.A., Granulocyte-macrophage colony-stimulating factor delays neutrophil constitutive apoptosis through phosphoinositide 3-kinase and extracellular signal-regulated kinase pathways (2000) J Immunol, 164, pp. 4286-4291
  • Ward, C., Chilvers, E.R., Lawson, M.F., NF-kappaB activation is a critical regulator of human granulocyte apoptosis in vitro (1999) J Biol Chem, 274, pp. 4309-4318
  • Sabroe, I., Prince, L.R., Jones, E.C., Selective roles for Toll-like receptor (TLR)2 and TLR4 in the regulation of neutrophil activation and life span (2003) J Immunol, 170, pp. 5268-5275
  • Jozsef, L., Khreiss, T., Filep, J.G., CpG motifs in bacterial DNA delay apoptosis of neutrophil granulocytes (2004) FASEB J, 18, pp. 1776-1778
  • Liaudet, L., Szabo, C., Evgenov, O.V., Flagellin from Gram-negative bacteria is a potent mediator of acute pulmonary inflammation in sepsis (2003) Shock, 19, pp. 131-137
  • Smith, K.D., Andersen-Nissen, E., Hayashi, F., Toll-like receptor 5 recognizes a conserved site on flagellin required for protofilament formation and bacterial motility (2003) Nat Immunol, 4, pp. 1247-1253
  • Lu, W., Hisatsune, A., Koga, T., Cutting edge: Enhanced pulmonary clearance of Pseudomonas aeruginosa by Muc1 knockout mice (2006) J Immunol, 176, pp. 3890-3894
  • Iino, T., Genetics and chemistry of bacterial flagella (1969) Bacteriol Rev, 33, pp. 454-475
  • Mizel, S.B., Snipes, J.A., Gram-negative flagellin-induced self-tolerance is associated with a block in interleukin-1 receptor-associated kinase release from toll-like receptor 5 (2002) J Biol Chem, 277, pp. 22414-22420
  • Capodici, C., Hanft, S., Feoktistov, M., Phosphatidylinositol 3-kinase mediates chemoattractant-stimulated, CD11b/CD18-dependent cell-cell adhesion of human neutrophils: Evidence for an ERK-independent pathway (1998) J Immunol, 160, pp. 1901-1909
  • Liew, F.Y., Xu, D., Brint, E.K., Negative regulation of Toll-like receptor-mediated immune responses (2005) Nat Rev Immunol, 5, pp. 446-458
  • Komoriya, K., Shibano, N., Higano, T., Flagellar proteins and type III-exported virulence factors are the predominant proteins secreted into the culture media of Salmonella typhimurium (1999) Mol Microbiol, 34, pp. 767-779
  • Rochon, M., Romling, U., Flagellin in combination with curli fimbriae elicits an immune response in the gastrointestinal epithelial cell line HT-29 (2006) Microbes Infect, 8, pp. 2027-2033
  • Rumbo, M., Nempont, C., Kraehenbuhl, J.P., Mucosal interplay among commensal and pathogenic bacteria: Lessons from flagellin and Tolllike receptor 5 (2006) FEBS Lett, 580, pp. 2976-2984
  • Subramanian, N., Qadri, A., Lysophospholipid sensing triggers secretion of flagellin from pathogenic salmonella (2006) Nat Immunol, 7, pp. 583-589
  • Gewirtz, A.T., TLRs in the Gut. III. Immune responses to flagellin in Crohn's disease: Good, bad or irrelevant? (2007) Am J Physiol Gastrointest Liver Physiol, 292, pp. G706-G710

Citas:

---------- APA ----------
Salamone, G.V., Petracca, Y., Bass, J.I.F., Rumbo, M., Nahmod, K.A., Gabelloni, M.L., Vermeulen, M.E.,..., Trevani, A.S. (2010) . Flagellin delays spontaneous human neutrophil apoptosis. Laboratory Investigation, 90(7), 1049-1059.
http://dx.doi.org/10.1038/labinvest.2010.77
---------- CHICAGO ----------
Salamone, G.V., Petracca, Y., Bass, J.I.F., Rumbo, M., Nahmod, K.A., Gabelloni, M.L., et al. "Flagellin delays spontaneous human neutrophil apoptosis" . Laboratory Investigation 90, no. 7 (2010) : 1049-1059.
http://dx.doi.org/10.1038/labinvest.2010.77
---------- MLA ----------
Salamone, G.V., Petracca, Y., Bass, J.I.F., Rumbo, M., Nahmod, K.A., Gabelloni, M.L., et al. "Flagellin delays spontaneous human neutrophil apoptosis" . Laboratory Investigation, vol. 90, no. 7, 2010, pp. 1049-1059.
http://dx.doi.org/10.1038/labinvest.2010.77
---------- VANCOUVER ----------
Salamone, G.V., Petracca, Y., Bass, J.I.F., Rumbo, M., Nahmod, K.A., Gabelloni, M.L., et al. Flagellin delays spontaneous human neutrophil apoptosis. Lab. Invest. 2010;90(7):1049-1059.
http://dx.doi.org/10.1038/labinvest.2010.77