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Abstract:

Capacitation of spermatozoa, a complex sequence of events that render them able to fertilize the egg, is generally associated with a switch from lineal, progressive movement to a vigorous, non-progressive pattern characterized by starlike tracks, a process known as hyperactivation. Development of a method for the analysis of progressive and hyperactive tracks is thus important for the assessment of capacitation in biochemical, physiological and clinical studies. In this study, we have applied a two- step heuristic model to deduce a lineal equation that discriminates hyperactive from progressive spermatozoa. The kinetic parameters (curvilinear velocity (VCL), linearity (LIN), amplitude of lateral head displacement (ALH), straightness (STR), wobble (WOB), mean 'dance' (DAN) and velocity of the average path (VAP)) of ram spermatozoa were evaluated with a computerized motility analyzer, and classified one by one as progressive or hyperactive by the appearance of their tracks. In a first step, a discriminating plane was defined by minimizing the number of misclassified spermatozoa ('conflicting points'); then, the plane was adjusted by an iterative process to minimize the distance from conflicting points to it. The resulting plane showed a discriminating capacity of over 95% for both classes, higher than that achieved by setting a threshold value for the parameters taken separately or in group. When included in a standard semen analysis, application of the equation allowed a rapid assessment of the percentage of hyperactive spermatozoa. The method described, developed in ram spermatozoa, can be applied to different species for a variety of purposes.

Registro:

Documento: Artículo
Título:A lineal equation for the classification of progressive and hyperactive spermatozoa
Autor:Vulcano, G.J.; Moses, D.F.; Valcárcel, A.; De Las Heras, M.A.
Filiación:Ctro. Invest. Reproductivas Perez C., Av. de Mayo 701-Piso 14, 1084, Buenos Aires, Argentina
Departamento de Computación, FCEyN, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:article; fertilization; mathematical analysis; mathematical model; semen analysis; spermatozoon capacitation; spermatozoon maturation; spermatozoon migration; spermatozoon motility; spermatozoon penetration; Animals; Kinetics; Linear Models; Male; Mathematics; Sheep; Sperm Capacitation; Sperm Motility; Spermatozoa
Año:1998
Volumen:149
Número:1
Página de inicio:77
Página de fin:93
DOI: http://dx.doi.org/10.1016/S0025-5564(97)10018-9
Título revista:Mathematical Biosciences
Título revista abreviado:Math. Biosci.
ISSN:00255564
CODEN:MABIA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00255564_v149_n1_p77_Vulcano

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Citas:

---------- APA ----------
Vulcano, G.J., Moses, D.F., Valcárcel, A. & De Las Heras, M.A. (1998) . A lineal equation for the classification of progressive and hyperactive spermatozoa. Mathematical Biosciences, 149(1), 77-93.
http://dx.doi.org/10.1016/S0025-5564(97)10018-9
---------- CHICAGO ----------
Vulcano, G.J., Moses, D.F., Valcárcel, A., De Las Heras, M.A. "A lineal equation for the classification of progressive and hyperactive spermatozoa" . Mathematical Biosciences 149, no. 1 (1998) : 77-93.
http://dx.doi.org/10.1016/S0025-5564(97)10018-9
---------- MLA ----------
Vulcano, G.J., Moses, D.F., Valcárcel, A., De Las Heras, M.A. "A lineal equation for the classification of progressive and hyperactive spermatozoa" . Mathematical Biosciences, vol. 149, no. 1, 1998, pp. 77-93.
http://dx.doi.org/10.1016/S0025-5564(97)10018-9
---------- VANCOUVER ----------
Vulcano, G.J., Moses, D.F., Valcárcel, A., De Las Heras, M.A. A lineal equation for the classification of progressive and hyperactive spermatozoa. Math. Biosci. 1998;149(1):77-93.
http://dx.doi.org/10.1016/S0025-5564(97)10018-9