Artículo

Giovambattista, A.; Suescun, M.O.; Nessralla, C.C.D.L.; França, L.R.; Spinedi, E.; Calandra, R.S. "Modulatory effects of leptin on leydig cell function of normal and hyperleptinemic rats" (2003) Neuroendocrinology. 78(5):270-279
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Abstract:

Neonatal L-monosodium glutamate (MSG) administration in rats induces several neuroendocrine and metabolic disruptions. Leptin, the adipocyte product, modulates several neuroendocrine systems including the hypothalamic-pituitary- gonadal (HPG) axis in mammals. The aim of the present study was to determine whether MSG-induced chronic hyperleptinemia could play any relevant role in the hypogonadism developed by male rats when examined in adulthood. We found that 120-day-old MSG male rats displayed significant hyperleptinemia, hypogonadism, and undisturbed basic testis structure and spermatogenesis. In vitro studies in purified Leydig cells from normal (CTR) and MSG-damaged rats revealed that basal and human chorionic gonadotropin (hCG)-stimulated 17-hydroxy-progesterone (17-HO-P4,) Δ4-androstenedione (Δ 4A) and testosterone (T) secretions were significantly lower in MSG than in CTR cells. Exposure to murine leptin (mleptin, 10-8 M) significantly inhibited hCG-elicited T secretion by CTR cells after 180 min incubation. While mleptin significantly inhibited hCG-stimulated Δ 4A output and the Δ4A:17-OH-P4 ratio of secretion, conversely, it failed to modify the ratio T:Δ4A release by CTR Leydig cells. Interestingly, the effects of mleptin found on CTR Leydig cells were absent in MSG Leydig cells. Finally, endogenous hyperleptinemia was associated with a significant decrease in Leydig cell expression of Ob-Rb mRNA in MSG rats. In summary, this study demonstrates that: (1) mleptin inhibited testicular steroidogenesis in CTR rats; (2) MSG-treated rats showed lower in vitro 17-OH-P4, Δ4A and T production under basal and post-hCG stimulation conditions; (3) purified Leydig cells from MSG-treated rats displayed resistance to the inhibitory action of mleptin on T release, and (4) endogenous leptin exerts a modulatory effect on Leydig cell Ob-Rb mRNA expression. The inhibitory effect of leptin on testicular function is thus abrogated in MSG-damaged rats. The testicular leptin-resistance developed by MSG rats seems to be due to early chronic exposure of Leydig cells to high leptin circulating levels, which in turn down-regulate testicular Ob-Rb expression. It remains to be determined whether the testicular dysfunction of MSG rats can be reversed after correction of hyperleptinemia or whether it is an irreversible effect of the hypothalamic lesion. Copyright © 2003 S. Karger AG, Basel.

Registro:

Documento: Artículo
Título:Modulatory effects of leptin on leydig cell function of normal and hyperleptinemic rats
Autor:Giovambattista, A.; Suescun, M.O.; Nessralla, C.C.D.L.; França, L.R.; Spinedi, E.; Calandra, R.S.
Filiación:Inst. Multidisciplinario Biol. Cel., La Plata, Argentina
Facultad de Ciencias Exactas, UNLP, La Plata, Argentina
Laboratory of Cell Biology, Federal University of Minas Gerais, Belo Horizonte, Brazil
Inst. de Biol. y Med. Experimental, Buenos Aires, Argentina
Inst. de Biol. y Med. Experimental, 1428 Buenos Aires, Argentina
Palabras clave:Gonadal steroids; Hypogonadism; Leptin; Leydig cells; Monosodium glutamate; Steroidogenesis; Testis; androstenedione; chorionic gonadotropin; glutamate sodium; hydroxyprogesterone; leptin; messenger RNA; testosterone; animal cell; animal experiment; animal model; animal tissue; article; cell function; controlled study; drug effect; female; hormone release; hyperleptinemia; hypogonadism; in vitro study; Leydig cell; male; neuroendocrine disease; nonhuman; priority journal; protein expression; rat; spermatogenesis; steroidogenesis; testis; testosterone release; Analysis of Variance; Androstenols; Animals; Animals, Newborn; Blotting, Northern; Body Weight; Disease Models, Animal; Dose-Response Relationship, Drug; Female; Follicle Stimulating Hormone; Hypogonadism; Leptin; Leydig Cells; Luteinizing Hormone; Male; Mice; Organ Size; Radioimmunoassay; Rats; Rats, Sprague-Dawley; Receptors, Cell Surface; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Sodium Glutamate; Testis; Testosterone; Thyroxine
Año:2003
Volumen:78
Número:5
Página de inicio:270
Página de fin:279
DOI: http://dx.doi.org/10.1159/000074448
Título revista:Neuroendocrinology
Título revista abreviado:Neuroendocrinology
ISSN:00283835
CODEN:NUNDA
CAS:androstenedione, 26264-53-9, 63-05-8; chorionic gonadotropin, 9002-61-3; glutamate sodium, 142-47-2, 16177-21-2, 16690-92-9; hydroxyprogesterone, 68-96-2; testosterone, 58-22-0; androsta-5,16-dien-3 beta-ol, 1224-94-8; Androstenols; Follicle Stimulating Hormone, 9002-68-0; leptin receptor; Leptin; Luteinizing Hormone, 9002-67-9; Receptors, Cell Surface; RNA, Messenger; Sodium Glutamate, 142-47-2; Testosterone, 58-22-0; Thyroxine, 7488-70-2
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00283835_v78_n5_p270_Giovambattista

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Citas:

---------- APA ----------
Giovambattista, A., Suescun, M.O., Nessralla, C.C.D.L., França, L.R., Spinedi, E. & Calandra, R.S. (2003) . Modulatory effects of leptin on leydig cell function of normal and hyperleptinemic rats. Neuroendocrinology, 78(5), 270-279.
http://dx.doi.org/10.1159/000074448
---------- CHICAGO ----------
Giovambattista, A., Suescun, M.O., Nessralla, C.C.D.L., França, L.R., Spinedi, E., Calandra, R.S. "Modulatory effects of leptin on leydig cell function of normal and hyperleptinemic rats" . Neuroendocrinology 78, no. 5 (2003) : 270-279.
http://dx.doi.org/10.1159/000074448
---------- MLA ----------
Giovambattista, A., Suescun, M.O., Nessralla, C.C.D.L., França, L.R., Spinedi, E., Calandra, R.S. "Modulatory effects of leptin on leydig cell function of normal and hyperleptinemic rats" . Neuroendocrinology, vol. 78, no. 5, 2003, pp. 270-279.
http://dx.doi.org/10.1159/000074448
---------- VANCOUVER ----------
Giovambattista, A., Suescun, M.O., Nessralla, C.C.D.L., França, L.R., Spinedi, E., Calandra, R.S. Modulatory effects of leptin on leydig cell function of normal and hyperleptinemic rats. Neuroendocrinology. 2003;78(5):270-279.
http://dx.doi.org/10.1159/000074448