Abstract:
Preincubation of synaptosome-rich homogenates of rat hypothalamus with pentoxifylline [3,7-dimethyl-1(5-oxo-hexyl)-xanthine] or theophylline resulted in significant decreases of norepinephrine, dopamine, serotonin and glutamate uptake. Methylxanthine inhibition was non-competitive; apparent Ki's ± S.E.M. for pentoxifylline and theophylline were 0.48 ± 0.03 and 0.84 ± 0.10 mM (norepinephrine), 0.20 ± 0.02 and 0.35 ± 0.05 mM (dopamine), 0.22 ± 0.03 and 0.98 ± 0.12 mM (serotonin) and 0.69 ± 0.08 and 1.13 ± 0.14 mM (glutamate). Methylxanthines did not affect the synaptosomal uptake of leucine, phenylalanine or alanine. Transmitter release evoked by increasing [K+] in the medium was augmented by pentoxifylline and theophylline in a dose-dependent manner. Dibutyryl cyclic AMP (1.0 mM) caused a significant increase of transmitter release following elevation of [K + ] in the medium but did not affect neurotransmitter uptake by hypothalamic synaptosomes. © 1977.
Registro:
Documento: |
Artículo
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Título: | Effects of pentoxifylline and theophylline on neurotransmitter uptake and release by synaptosome-rich homogenates of the rat hypothalamus |
Autor: | Cardinali, D.P. |
Filiación: | Centro de Estudios Farmacológicos y de Principios Naturales (C.E.F.A.P.R.I.N.), Consejo Nacional de Investigaciones Cientificas y Técnicas, Obligado 2490, 1428 Buenos Aires, Argentina
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Palabras clave: | adenosine-3',5'-monophosphate; amino acid uptake; dopamine uptake and release; hypothalamic synaptosomes; l-glutamate uptake and release; methylxanthines; norepinephrine uptake and release; pentoxifylline [3,7-dimethyl-1-(5-oxo-hexyl)-xanthine]; serotonin uptake and release; theophylline; bucladesine; dopamine; glutamic acid; methylxanthine; neurotransmitter; noradrenalin; pentoxifylline; potassium; radioisotope; serotonin; theophylline; dopamine h 3; dose response; drug comparison; drug response; glutamic acid h 3; hypothalamus; in vitro study; michaelis constant; noradrenalin h 3; rat; serotonin h 3; synaptosome; theoretical study; Animal; Bucladesine; Dopamine; Glutamates; Hypothalamus; Male; Neurotransmitters; Norepinephrine; Pentoxifylline; Potassium; Rats; Serotonin; Synaptosomes; Theobromine; Theophylline; Xanthines |
Año: | 1977
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Volumen: | 16
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Número: | 11
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Página de inicio: | 785
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Página de fin: | 790
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DOI: |
http://dx.doi.org/10.1016/0028-3908(77)90138-1 |
Título revista: | Neuropharmacology
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Título revista abreviado: | Neuropharmacology
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ISSN: | 00283908
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CODEN: | NEPHB
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CAS: | bucladesine, 16980-89-5, 362-74-3; dopamine, 51-61-6, 62-31-7; glutamic acid, 11070-68-1, 138-15-8, 56-86-0, 6899-05-4; methylxanthine, 28109-92-4; noradrenalin, 1407-84-7, 51-41-2; pentoxifylline, 6493-05-6; potassium, 7440-09-7; serotonin, 50-67-9; theophylline, 58-55-9, 5967-84-0, 8055-07-0, 8061-56-1, 99007-19-9; Bucladesine, 362-74-3; Dopamine, 51-61-6; Glutamates; Neurotransmitters; Norepinephrine, 51-41-2; Pentoxifylline, 6493-05-6; Potassium, 7440-09-7; Serotonin, 50-67-9; Theobromine, 83-67-0; Theophylline, 58-55-9; Xanthines
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00283908_v16_n11_p785_Cardinali |
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Citas:
---------- APA ----------
(1977)
. Effects of pentoxifylline and theophylline on neurotransmitter uptake and release by synaptosome-rich homogenates of the rat hypothalamus. Neuropharmacology, 16(11), 785-790.
http://dx.doi.org/10.1016/0028-3908(77)90138-1---------- CHICAGO ----------
Cardinali, D.P.
"Effects of pentoxifylline and theophylline on neurotransmitter uptake and release by synaptosome-rich homogenates of the rat hypothalamus"
. Neuropharmacology 16, no. 11
(1977) : 785-790.
http://dx.doi.org/10.1016/0028-3908(77)90138-1---------- MLA ----------
Cardinali, D.P.
"Effects of pentoxifylline and theophylline on neurotransmitter uptake and release by synaptosome-rich homogenates of the rat hypothalamus"
. Neuropharmacology, vol. 16, no. 11, 1977, pp. 785-790.
http://dx.doi.org/10.1016/0028-3908(77)90138-1---------- VANCOUVER ----------
Cardinali, D.P. Effects of pentoxifylline and theophylline on neurotransmitter uptake and release by synaptosome-rich homogenates of the rat hypothalamus. Neuropharmacology. 1977;16(11):785-790.
http://dx.doi.org/10.1016/0028-3908(77)90138-1