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Abstract:

Immature stages of Ceratitis capitata were tested as a model for hematoporphyrin IX (HP IX) phototoxicity. The lethal concentration 50 (LC 50) of HP IX in the food was determined during postembryonic development until adult emergence as 0.173 mm (95% CI: 0.138-0.209). The corresponding HP IX LC50 during the dispersal period alone was 0.536 mm (95% CI: 0.450-0.633). HP IX toxicity was compared against Phloxine B (PhB) (0.5 mm). HP IX elicited a mortality of 90.87%, which was mainly concentrated during prepupal and early pupal stages. PhB mortality was much lower (56.88%) and occurred mainly during the adult pharate stage. A direct correlation between light-dependent HP IX mortality, evidence of reactive oxygen species (ROS) and lipid peroxidation (conjugated dienes and thiobarbituric acid reactive substances) was established in C. capitata larvae. ROS were found to be very significant in both the brain and in the gut.

Registro:

Documento: Artículo
Título:Photosensitizing effect of hematoporphyrin IX on immature stages of ceratitis capitata (Diptera: Tephritidae)
Autor:Pujol-Lereis, L.M.; Massaldi, A.; Rabossi, A.; Quesada-Allué, L.A.
Filiación:IIBBA-CONICET, Química Biológica-FCEyN-Universidad de Buenos Aires, Fundación Instituto Leloir, Buenos Aires, Argentina
Palabras clave:hematoporphyrin; photosensitizing agent; reactive oxygen metabolite; thiobarbituric acid; thiobarbituric acid derivative; hematoporphyrin; animal; article; brain; drug effect; intestine; larva; lipid peroxidation; Mediterranean fruit fly; metabolism; radiation exposure; drug effects; Mediterranean fruit fly; radiation response; Animals; Brain; Ceratitis capitata; Hematoporphyrins; Intestines; Larva; Lipid Peroxidation; Photosensitizing Agents; Reactive Oxygen Species; Thiobarbiturates; Ceratitis capitata; Diptera; Tephritidae; Animals; Brain; Ceratitis capitata; Hematoporphyrins; Intestines; Larva; Lipid Peroxidation; Photosensitizing Agents; Reactive Oxygen Species; Thiobarbiturates
Año:2010
Volumen:86
Número:3
Página de inicio:639
Página de fin:644
DOI: http://dx.doi.org/10.1111/j.1751-1097.2009.00704.x
Título revista:Photochemistry and Photobiology
Título revista abreviado:Photochem. Photobiol.
ISSN:00318655
CODEN:PHCBA
CAS:hematoporphyrin, 14459-29-1; thiobarbituric acid, 504-17-6; Hematoporphyrins; Photosensitizing Agents; Reactive Oxygen Species; Thiobarbiturates; thiobarbituric acid, 504-17-6; Hematoporphyrins; Photosensitizing Agents; Reactive Oxygen Species; Thiobarbiturates; thiobarbituric acid
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00318655_v86_n3_p639_PujolLereis

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Citas:

---------- APA ----------
Pujol-Lereis, L.M., Massaldi, A., Rabossi, A. & Quesada-Allué, L.A. (2010) . Photosensitizing effect of hematoporphyrin IX on immature stages of ceratitis capitata (Diptera: Tephritidae). Photochemistry and Photobiology, 86(3), 639-644.
http://dx.doi.org/10.1111/j.1751-1097.2009.00704.x
---------- CHICAGO ----------
Pujol-Lereis, L.M., Massaldi, A., Rabossi, A., Quesada-Allué, L.A. "Photosensitizing effect of hematoporphyrin IX on immature stages of ceratitis capitata (Diptera: Tephritidae)" . Photochemistry and Photobiology 86, no. 3 (2010) : 639-644.
http://dx.doi.org/10.1111/j.1751-1097.2009.00704.x
---------- MLA ----------
Pujol-Lereis, L.M., Massaldi, A., Rabossi, A., Quesada-Allué, L.A. "Photosensitizing effect of hematoporphyrin IX on immature stages of ceratitis capitata (Diptera: Tephritidae)" . Photochemistry and Photobiology, vol. 86, no. 3, 2010, pp. 639-644.
http://dx.doi.org/10.1111/j.1751-1097.2009.00704.x
---------- VANCOUVER ----------
Pujol-Lereis, L.M., Massaldi, A., Rabossi, A., Quesada-Allué, L.A. Photosensitizing effect of hematoporphyrin IX on immature stages of ceratitis capitata (Diptera: Tephritidae). Photochem. Photobiol. 2010;86(3):639-644.
http://dx.doi.org/10.1111/j.1751-1097.2009.00704.x