Extraction with alkali of the seeds of Gleditsia triacanthos, after exhaustive extraction with water and neutralization of the extracts by dialysis, permits isolation of the 75% ethanol-soluble products. Gel permeation chromatography of these products allows isolation of an homogeneous galactomannan-protein complex which was further purified by anion-exchange chromatography. The galactomannan has a medium degree of polymerization and a backbone similar to that of the classical galactomannans. The main difference with these polysaccharides lies in the side-chains which are attached to the backbone not only by (1 → 6)-linkages but also by (1 → 3)-linkages and which contain, on average, two galactose units, either (1 → 3), (1 → 6)-linked or (1 → 3), (1 → 3)-linked with α-d and β-d configurations, respectively. © 1986.
Documento: | Artículo |
Título: | The alkali-soluble galactomannan-like oligosaccharides from the seed of Gleditsia triacanthos |
Autor: | Mazzini, M.N.; Cerezo, A.S. |
Filiación: | Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, 1428 Buenos Aires, Argentina |
Año: | 1986 |
Volumen: | 6 |
Número: | 3 |
Página de inicio: | 203 |
Página de fin: | 212 |
DOI: | http://dx.doi.org/10.1016/0144-8617(86)90020-2 |
Título revista: | Carbohydrate Polymers |
Título revista abreviado: | Carbohydr Polym |
ISSN: | 01448617 |
CODEN: | CAPOD |
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01448617_v6_n3_p203_Mazzini |