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Abstract:

We have previously shown that in the ascomycete Saccobolus platensis the presence of cyclic AMP can overcome the requirement for light to produce apothecia. With the aim to gain insight into the phototransduction mechanism the existence of G protein-like molecules was investigated. By using the GTP analog [35S]GTP-γS we detected the existence of GTP-binding proteins; the dissociation constant for the binding of the nucleotide was calculated (Kd = 52 nM); the binding was effectively competed by GTP and GDPPS and to a lesser extent by ATP. We also demonstrated by SDS-PAGE the existence of proteins of 48, 41, 26, and 15 kDa that are substrates of the cholera toxin-dependent ADP-ribosylation reaction. Proteins resolved by SDS-PAGE were identified by Western blotting with antisera specific for vertebrate G protein subunits; the antibody anti-αcommon revealed two main bands at 48 and 41 kDa; the AS/7 antibody (anti-αt, −α1, and −ai2) led to the identification of one band at 41 kDa; the antibody anti-β subunit revealed a polipeptide band at around 37 kDa. The antibody anti-αcommon also abolished the binding of [35S]GTP-γS to crude membranes. The results presented in this paper provide evidence for the existence in S. platensis of proteins that proved to have a behavior similar to that of mammalian G proteins. © 1994, Academic Press, Inc.. All rights reserved.

Registro:

Documento: Artículo
Título:Occurrence of GTP-binding proteins in the ascomycete Saccobolus platensis
Autor:Zelada, A.M.; Samela, A.; Passeron, S.; Cantors, M.L.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón II, Buenos Aires, 1428, Argentina
CONICET, Argentina
Palabras clave:ascomycete; GTP-binding proteins; photodifferentiation; Saccobolus platensis; adenosine triphosphate; cholera toxin; guanine nucleotide binding protein; guanosine triphosphate; protein subunit; sulfur 35; article; Ascomycetes; binding competition; dissociation constant; immunoblotting; membrane binding; nonhuman; polyacrylamide gel electrophoresis; priority journal; Ascomycota; Mammalia; Saccobolus; Vertebrata
Año:1994
Volumen:18
Número:4
Página de inicio:330
Página de fin:337
DOI: http://dx.doi.org/10.1016/S0147-5975(06)80006-X
Título revista:Experimental Mycology
Título revista abreviado:Exp. Mycol.
ISSN:01475975
CAS:adenosine triphosphate, 15237-44-2, 56-65-5, 987-65-5; guanosine triphosphate, 86-01-1; sulfur 35, 15117-53-0
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01475975_v18_n4_p330_Zelada

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Citas:

---------- APA ----------
Zelada, A.M., Samela, A., Passeron, S. & Cantors, M.L. (1994) . Occurrence of GTP-binding proteins in the ascomycete Saccobolus platensis. Experimental Mycology, 18(4), 330-337.
http://dx.doi.org/10.1016/S0147-5975(06)80006-X
---------- CHICAGO ----------
Zelada, A.M., Samela, A., Passeron, S., Cantors, M.L. "Occurrence of GTP-binding proteins in the ascomycete Saccobolus platensis" . Experimental Mycology 18, no. 4 (1994) : 330-337.
http://dx.doi.org/10.1016/S0147-5975(06)80006-X
---------- MLA ----------
Zelada, A.M., Samela, A., Passeron, S., Cantors, M.L. "Occurrence of GTP-binding proteins in the ascomycete Saccobolus platensis" . Experimental Mycology, vol. 18, no. 4, 1994, pp. 330-337.
http://dx.doi.org/10.1016/S0147-5975(06)80006-X
---------- VANCOUVER ----------
Zelada, A.M., Samela, A., Passeron, S., Cantors, M.L. Occurrence of GTP-binding proteins in the ascomycete Saccobolus platensis. Exp. Mycol. 1994;18(4):330-337.
http://dx.doi.org/10.1016/S0147-5975(06)80006-X