Artículo

Ruiz, M.D.; Iriel, A.; Yusseppone, M.S.; Ortiz, N.; Di Salvatore, P.; Fernández Cirelli, A.; Ríos de Molina, M.C.; Calcagno, J.A.; Sabatini, S.E. "Trace metals and oxidative status in soft tissues of caged mussels (Aulacomya atra) on the North Patagonian coastline" (2018) Ecotoxicology and Environmental Safety. 155:152-161
El editor solo permite decargar el artículo en su versión post-print desde el repositorio. Por favor, si usted posee dicha versión, enviela a
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

This study investigated metal accumulation and oxidative effects in mantle, gill and digestive gland of the ribbed mussel Aulacomya atra from the Argentinean North Patagonian coastline. Mussels were transplanted over an 18-month period from a site with low anthropogenic impact to a harbor site with higher seawater concentration of aluminum, chromium, copper, manganese, nickel and zinc. Total trace metal concentration in seawater did not change throughout the 18-month transplant in either site. A. atra bioaccumulated metals in digestive gland, gills and mantle at different levels. Digestive gland had the highest concentration of metals, especially towards the end of the transplant experiment in the harbor area. Mussels transplanted to the harbor site experienced an upregulation in their antioxidant system, which likely explains the lack of oxidative damage to lipids despite higher metal accumulation. These results demonstrate that A. atra selectively accumulates metals from the water column and their prooxidant effects depend on the tissue antioxidant defenses and the exposure time. © 2018 Elsevier Inc.

Registro:

Documento: Artículo
Título:Trace metals and oxidative status in soft tissues of caged mussels (Aulacomya atra) on the North Patagonian coastline
Autor:Ruiz, M.D.; Iriel, A.; Yusseppone, M.S.; Ortiz, N.; Di Salvatore, P.; Fernández Cirelli, A.; Ríos de Molina, M.C.; Calcagno, J.A.; Sabatini, S.E.
Filiación:Instituto de Ciencia y Tecnología Dr. Cesar Milstein, (CONICET), Saladillo 2468, Ciudad Autónoma de Buenos Aires, C1440FFX, Argentina
Instituto de Investigaciones en Producción Animal / INPA(UBA-CONICET) / Centro de Estudios Transdisciplinarios del Agua (CETA), Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Av. Chorroarín 280, Ciudad Autónoma de Buenos Aires, C1427CWO, Argentina
Instituto de Investigaciones Marinas y Costeras (IIMyC), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Mar del Plata (UNMDP), CC 1260 Correo Central (B7600WAG), Mar del Plata, Argentina
Instituto de Biología de Organismos Marinos (IBIOMAR – CCT CONICET–CENPAT), Blvd. Brown 2915, Puerto Madryn, 9120, Argentina
Universidad Tecnológica Nacional - Facultad Regional Chubut, Av. del Trabajo 1536, Puerto Madryn, 9120, Argentina
Centro Austral de Investigaciones Científicas (CADIC)-CONICET, Houssay 200, Ushuaia, V9410CAB, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pab. II, Intendente Guiraldes 2160, Ciudad Autónoma de Buenos Aires, C1428EHA, Argentina
CONICET, Universidad de Buenos Aires. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN), Pab. II, Intendente Guiraldes 2160, Ciudad Autónoma de Buenos Aires, C1428EHA, Argentina
Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico (CEBBAD)- Departamento de Ciencias Naturales y Antropológicas-Universidad Maimonides, Hidalgo 775, Ciudad Autónoma de Buenos Aires, C1405BCK, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina
Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pab. II, Intendente Guiraldes 2160, Ciudad Autónoma de Buenos Aires, C1428EHA, Argentina
Palabras clave:Antioxidant system; Lipid peroxidation; Metal bioaccumulation; ROS; TOSC; Transplanted mussels; aluminum; chromium; copper; glutathione; manganese; nickel; reactive oxygen metabolite; sea water; superoxide dismutase; zinc; metal; sea water; antioxidant; bioaccumulation; bivalve; coast; harbor; heavy metal; lipid; oxidative stress; trace metal; animal tissue; Article; assay; Aulacomya atra; bioaccumulation; concentration (parameters); controlled study; enzyme activity; exocrine gland; gill; limit of quantitation; lipid peroxidation; mantle (mollusc); measurement accuracy; measurement precision; Mytilidae; nonhuman; oxidative stress; protein content; total oxyradical scavenging capacity assay; upregulation; water temperature; analysis; animal; Argentina; environmental monitoring; gastrointestinal tract; gill; metabolism; Mytilidae; oxidative stress; water pollutant; Argentina; Patagonia; Aulacomya ater; Geukensia demissa; Animals; Argentina; Environmental Monitoring; Gastrointestinal Tract; Gills; Metals; Mytilidae; Oxidative Stress; Seawater; Water Pollutants, Chemical
Año:2018
Volumen:155
Página de inicio:152
Página de fin:161
DOI: http://dx.doi.org/10.1016/j.ecoenv.2018.02.064
Título revista:Ecotoxicology and Environmental Safety
Título revista abreviado:Ecotoxicol. Environ. Saf.
ISSN:01476513
CODEN:EESAD
CAS:aluminum, 7429-90-5; chromium, 16065-83-1, 7440-47-3, 14092-98-9; copper, 15158-11-9, 7440-50-8; glutathione, 70-18-8; manganese, 16397-91-4, 7439-96-5; nickel, 7440-02-0; superoxide dismutase, 37294-21-6, 9016-01-7, 9054-89-1; zinc, 7440-66-6, 14378-32-6; Metals; Water Pollutants, Chemical
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01476513_v155_n_p152_Ruiz

Referencias:

  • Amado, L., Garcia, L., Ramos, P., Freitas, R., Zafalon, B., Ribas Ferreira, J., A method to measure total antioxidant capacity against peroxyl radicals in aquatic organisms: application to evaluate microcystins toxicity (2009) Sci. Total Environ., 40, pp. 2115-2123
  • Anderson, M.E., Determination of glutathione and glutathione disulfide in biological samples (1985) Methods Enzymol., 113, pp. 548-553
  • Andral, B., Stanisiere, J.Y., Sauzade, D., Damier, E., Thebault, H., Galgani, F., Boissery, P., Monitoring chemical contamination levels in the Mediterranean based on the use of mussel caging (2004) Mar. Poll. Bull., 49, pp. 704-712
  • Attig, H., Dagnino, A., Negri, A., Jebali, J., Boussetta, H., Viarengo, A., Dondero, F., Banni, M., Uptake and biochemical responses of mussels Mytilus galloprovincialis exposed to sublethal nickel concentrations (2010) Ecotox. Environ. Saf., 73, pp. 1712-1719
  • Banni, M., Sforzini, S., Franzellitti, S., Oliveri, C., Viarengo, A., Fabbri, E., Molecular and cellular effects induced in Mytilus galloprovincialis treated with oxytetracycline at different temperatures (2015) PLoS One, 10 (6), p. e0128468
  • Baqueiro-Cárdenas, E.R., Borabe, L., Goldaracena-Islas, C.G., Rodríguez-Navarro, J., Los moluscos y la contaminación (2007) Una Rev. Rev. Mex. De. Biodivers., 78, pp. 1S-7S
  • Beauchamp, C., Fridovich, I., Superoxide dismutase: improved assays and an assay applicable to polyacrylamide gels (1971) Anal. Biochem., 44, pp. 276-286
  • Belcheva, N.N., Zakhartsev, M.V., Dovzhenko, N.V., Zhukovskaya, A.F., Kavun, V.Y., Chelomin, V.P., Anthropogenic pollution stimulates oxidative stress in soft tissues of mussel Crenomytilus grayanus (Dunker1853) (2011) Ocean Sci. J., 46 (2), pp. 85-94
  • Benedetti, M., Giuliani, M.E., Regoli, F., Oxidative metabolism of chemical pollutants in marine organisms: molecular and biochemical biomarkers in environmental toxicology (2015) Ann. N. Y. Acad. Sci., 1340, pp. 8-19
  • Beuge, J.A., Aust, S.D., Microsomal lipid peroxidation (1978) Methods Enzymol., 52, pp. 302-310
  • Bocchetti, R., Lamberti, C.V., Pisanelli, B., Razzetti, E.M., Maggi, C., Catalano, B., Sesta, G., Regoli, F., Seasonal variations of exposure biomarkers, oxidative stress responses and cell damage in the clams. Tapes philippinarum, and mussels, Mytilus galloprovincialis, from Adriatic sea (2008) Mar. Environ. Res., 66, pp. 24-26
  • Box, A., Sureda, A., Galgani, F., Pons, A., Deudero, S., Assessment of environmental pollution at Balearic Islands applying oxidative stress biomarkers in the mussel Mytilus galloprovincialis (2007) Comp. Biochem. Physiol. C Toxicol. Pharmacol., 146 (4), pp. 531-539
  • Bradford, M.M., A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein dye binding (1976) Anal. Biochem., 72, pp. 248-254
  • Breitwieser, M., Viricel, A., Graber, M., Murillo, L., Becquet, V., Churlaud, C., Fruitier-Arnaudin, I., Thomas-Guyon, H., Short-term and long-term biological effects of chronic chemical contamination on natural populations of a marine bivalve (2016) PLoS One, 11, pp. 1-14
  • Canesi, L., Viarengo, A., Leonzio, C., Filippelli, M., Gallo, G., Heavy metals and glutathione metabolism in mussel tissues (1999) Aquat. Toxicol., 46, pp. 67-76
  • Cappello, T., Maisano, M., D'Agata, A., Natalotto, A., Mauceri, A., Fasulo, S., Effects of environmental pollution in caged mussels (Mytilus galloprovincialis) (2013) Mar. Environ. Res., 91, pp. 52-60
  • Cappello, T., Maisano, M., Giannetto, A., Parrino, V., Mauceri, A., Fasulo, S., Neurotoxicological effects on marine mussel Mytilus galloprovincialis caged at petrochemical contaminated areas (eastern Sicily, Italy): 1 H NMR and immunohistochemical assays (2015) Comp. Biochem. Physiol. C, 169, pp. 7-15
  • Censi, P., Spoto, S.E., Saiano, F., Sprovieri, M., Mazzola, S., Nardone, G., Heavy metals in coastal water systems. A case study from the northwestern Gulf of Thailand (2006) Chemosphere, 64, pp. 1167-1176
  • Chandurvelan, R., Marsden, I.D., Glover, C.N., Gaw, S., Assessment of a mussel as a metal bioindicator of coastal contamination: relationships between metal bioaccumulation and multiple biomarker responses (2015) Sci. Total Environ., 511, pp. 663-675
  • Ciacci, C., Barmo, C., Gallo, G., Maisano, M., Cappello, T., D'Agata, A., Leonzio, C., Canesia, L., Effects of sublethal, environmentally relevant concentrations of hexavalent chromium in the gills of Mytilus galloprovincialis (2012) Aquat. Toxicol., 120-121, pp. 109-118
  • Cohen, J.E., Small, C., Mellinger, A., Gallup, J., Sachs, J., Estimates of coastal populations (1997) Science, 278, pp. 1211-1212
  • Commendatore, M.G., Esteves, J.L., An assessment of oil pollution in the coastal zone of Patagonia, Argentina (2007) Environ. Manag., 40, pp. 814-821
  • Cossu, C., Doyotte, A., Babut, M., Exinger, A., Vasseur, P., Antioxidant biomarkers in freshwater bivalves, Uniotumidus, in response to different contamination profiles of aquatic sediments (2000) Ecotox. Environ. Saf., 45, pp. 106-121
  • Dafre, A.L., Medeiros, I.D., Muller, I.C., Ventura, E.C., Bainy, A.C.D., Antioxidant enzymes and thiol/disulfide status in the digestive gland of the brown mussel Perna perna exposed to lead and paraquat (2004) Chem. Biol. Interact., 149, pp. 97-105
  • Dailianis, S., Piperakis, S., Kaloyianni, M., Cadmium effects on ROS production and DNA damage via adrenergic receptors stimulation: role of Na1/H1 exchanger and PKC (2005) Free Radic. Res., 39, pp. 1059-1070
  • de Almeida, E.A., Miyamoto, S., Dias Bainy, A.C., Gennari de Medeiros, M.H., Di Mascio, P., Protective effect of phospholipid hydroperoxide glutathione peroxidase (PHGPx) against lipid peroxidation in mussels Perna perna exposed to different metals (2004) Mar. Poll. Bull., 49 (5-6), pp. 386-392
  • de Souza Machado, A.A., Spencer, K., Kloas, W., Toffolon, M., Zarfl, C., Metal fate and effects in estuaries: a review and conceptual model for better understanding of toxicity (2016) Sci. Total Environ., 541, pp. 268-281
  • Di Salvatore, P., Calcagno, J.A., Ortíz, N., Ríos de Molina, M.C., Sabatini, S.E., Effect of seasonality on oxidative stress responses and metal accumulation in soft tissues of Aulacomya atra from the South Atlantic Patagonian coast (2013) Mar. Environ. Res., 92, pp. 244-252
  • Eberhardt, M.K., In Reactive Oxygen Metabolites, Chemistry and Medical Consequences (2001), CRC Press NY, USA; Esteves, J.L., (2008), http://atlas.ambiente.gov.ar/tematicas/mt_04/especies.htm, Contaminación costera marina. Metales pesados. In: Atlas de Sensibilidad Ambiental de la Costa y el Mar Argentino (Boltovskoy D Ed.); Fasulo, S., Iacono, F., Cappello, T., Corsaro, C., Maisano, M., D'Agata, A., Giannetto, A., Maureci, A., Metabolomic investigation of Mytilus galloprovincialis (Lamarck1819) caged in aquatic environments (2012) Ecotoxicol. Environ. Saf., 84, pp. 139-146
  • Frenzilli, G., Bocchetti, R., Pagliarecci, M., Nigro, M., Annarumma, F., Scarcelli, V., Fattorini, D., Regoli, F., Time-course evaluation of ROS-mediated toxicity in mussels, Mytilus galloprovincialis, during a field translocation experiment (2004) Mar. Environ. Res., 58, pp. 609-613
  • Führer, E., Rudolph, A., Espinoza, C., Díaz, R., Gajardo, M., Camaño, N., Integrated use of biomarkers (O:N ratio and acetylcholinesterase inhibition) on Aulacomya ater (Molina, 1782) (Bivalvia: Mytilidae) as a criteria for effects of organophosphate pesticide exposition (2012) J. Toxicol., 2012. , (Article ID951568)
  • Giarratano, E., Gil, M.N., Malanga, G., Biomarkers of environmental stress in gills of ribbed mussel Aulacomya atra atra (Nuevo Gulf, Northern Patagonia) (2014) Ecotoxicol. Environ. Saf., 107, pp. 111-119
  • Gil, M.N., Harvey, M.A., Esteves, J.L., Heavy metals in intertidal sediments from Patagonian coast, Argentina (1999) Bull. Environ. Cont. Toxicol., 63, pp. 52-58
  • Gorinstein, S., Moncheva, S., Katrich, E., Toledo, F., Arancibia, P., Goshev, I., Trakhtenberg, S., Antioxidants in the black mussel (Mytilus galloprovincialis) as an indicator of black sea coastal pollution (2003) Mar. Poll. Bull., 46, pp. 1317-1325
  • Guzmán, N., Sáa, S., Ortlieb, L., Catálogo descriptivo de los moluscos litorales (Gastropoda y Pelecypoda) de la zona de Antofagasta (Chile) (1998) Estud. Oceanol., 17, pp. 17-86
  • Habig, W.H., Pabst, M.J., Jakoby, W.B., Glutathione S-transferases: the first enzymatic step in mercapturic acid formation (1974) J. Biol. Chem., 249, pp. 7130-7139
  • Jaishankar, M., Tseten, T., Anbalagan, N., Mathew, B.B., Beeregowda, K.N., Toxicity, mechanism and health effects of some heavy metals (2014) Interdiscip. Toxicol., 7 (2), pp. 60-72
  • Kumari, K., Khare, A., Dange, S., The applicability of oxidative stress biomarkers in assessing chromium induced toxicity in the fish Labeo rohita (2014) Biomed. Res. Int., 2014, pp. 1-11
  • Lesser, M.P., Oxidative stress in marine environments: biochemistry and physiological ecology (2006) Annu. Rev. Physiol., 68, pp. 253-278
  • Livingstone, D.R., Contaminant stimulated reactive oxygen species production and oxidative damage in aquatic organisms (2001) Mar. Pollut. Bull., 42, pp. 656-666
  • Lloyd, D.R., Phillips, D.H., Oxidative DNA damage mediated by copper (II), iron(II) and nickel(II) Fenton reactions: evidence for site-specific mechanisms in the formation of double-strand breaks, 8-hydroxydeoxyguanosine and putative intrastrand cross-links (1999) Mut. Res., 424, pp. 23-36
  • Luoma, S.N., Rainbow, P.S., Why is metal bioaccumulation so variable? Biodynamics as a unifying concept (2005) Environ. Sci. Technol., 39, pp. 1921-1931
  • Lushchak, V.I., Environmentally induced oxidative stress in aquatic animals (2011) Aquat. Toxicol., 101, pp. 13-30
  • Macfarlane, G.R., Burchett, M.D., Photosynthetic pigments and peroxidase activity as indicators of heavy metal stress in the Greymangrove, Avicennia marina (Forsk.) Vierh (2000) Mar. Pollut. Bull., 42, pp. 233-240
  • Marasinghe Wadige, C.P.M., Taylor, A.M., Krikowa, F., Lintermans, M., Maher, W.A., Exposure of the freshwater bivalve Hyridella australis to metal contaminated sediments in the field and laboratory microcosms: metal uptake and effects (2017) Ecotoxicol, 26 (3), pp. 415-434
  • Marigómez, I., Soto, M., Cajaraville, M.P., Angulo, E., Giamberini, L., Cellular and Subcellular distribution of metals in molluscs (2002) Microsc. Res. Technol., 56, pp. 358-392
  • Marigómez, I., Zorita, I., Izagirre, U., Ortiz-Zarragoitia, M., Navarro, P., Etxebarria, N., Orbea, A., Cajaraville, M.P., Combined use of native and caged mussels to assess biological e'ects of pollution through the integrative biomarker approach (2013) Aquat. Toxicol., 136, pp. 32-48
  • Martin, T.D., Brockhoff, C.A., Creed, J.T., Long, S.E., Determination of Metals and Trace Elements in Water and Wastes by Inductively Coupled Plasma-atomic Emission Spectrometry (1991), pp. 31-72. , Environmental Monitoring Systems Laboratory, Office of Research and Development, US Environmental Protection Agency Cincinnati, OH; Massara Paletto, V., Commendatore, M.G., Esteves, J.L., Hydrocarbon levels in sediments and bivalve mollusks from Bahía Nueva (Patagonia, Argentina): an assessment of probable origin and bioaccumulation factors (2008) Mar. Poll. Bull., 56, pp. 2100-2105
  • Mejdoub, Z., Fahde, A., Loutfi, M., Kabine, M., Oxidative stress responses of the mussel Mytilus galloprovincialis exposed to emissary's pollution in coastal areas of Casablanca (2017) O. Coast. Manag., 136, pp. 95-103
  • Miller, G.G., Sweet, L.I., Adams, J.V., Omann, G.M., Passino-Reader, D., Meter, P.G., In vitro toxicity and interactions of environmental contaminants (Arochlor 125 and mercury) and immunomodulatory agents (lipopolysaccharidae and cortisol) on thymocytes from lake trout (Salvelinus namaycush) (2002) Fish. Shellfish Immunol., 13, pp. 11-26
  • Mubiana, V.K., Blust, R., Effects of temperature on scope for growth and accumulation of Cd, Co, Cu and Pb by the marine bivalve Mytilus edulis (2007) Mar. Environ. Res., 63, pp. 219-235
  • Muniz, P., Venturini, N., Martins, C.C., Munshi, A.B., García-Rodríguez, F., Brugnoli, E., Lindroth Dauner, A.L., García-Alonso, J., Integrated assessment of contaminants and monitoring of an urbanized temperate harbor (Montevideo, Uruguay): a 12-year comparison (2015) Braz. J. Oceanogr., 63 (3), pp. 311-330
  • Narvarte, M., González, R., Filippo, P., Artisanal mollusk fisheries in San Matías Gulf (Patagonia, Argentina): an appraisal of the factors contributing to unsustainability (2007) Fish. Res., 87, pp. 68-76
  • Nasci, C., Nesto, N., Monteduro, R.A., Da Ros, L., Field application of biochemical markers and a physiological index in the mussel, Mytilus galloprovincialis: transplantation and biomonitoring studies in the lagoon of Venice (NE Italy) (2002) Mar. Environ. Res., 54 (3-5), pp. 811-816
  • Orensanz, J.M., Parma, A.M., Ciocco, N., Cinti, A., Achievements and setbacks in the commercial diving fishery of San José Gulf, Argentine Patagonia (2007) Fisheries Management: Progress Towards Sustainability, pp. 68-87. , T.R. McClanahan J.C. Castilla Blackwell Publishing Ltd
  • Pan, K., Tan, Q.G., Wang, W.X., Two-compartment kinetic modeling of radiocesium accumulation in marine bivalves under hypothetical exposure regimes (2016) Environ. Sci. Technol., 50 (5), pp. 2677-2684
  • Perić, L., Nerlović, V., Žurga, P., Žilić, L., Ramšak, A., Variations of biomarkers response in mussels Mytilus galloprovincialis to low, moderate and high concentrations of organic chemicals and metals (2017) Chemosphere, 174, pp. 554-562
  • Pilote, M., André, C., Turcotte, P., Gagné, F., Gagnon, C., Metal bioaccumulation and biomarkers of effects in caged mussels exposed in the Athabasca oil sands area (2017) Sci. Total Environ., 610-611, pp. 377-390
  • Rainbow, P.S., Trace metal concentrations in aquatic invertebrates: why and so what? (2002) Environ. Pollut., 120, pp. 497-507
  • Rainbow, P.S., Smith, B.D., Luoma, S.B., Biodynamic modelling and the prediction of Ag, Cd and Zn accumulation from solution and sediment by the polychaete Nereis diversicolor (2009) Mar. Ecol. Prog. Ser., 390, pp. 145-155
  • Rajkumar, J.S.I., John Milton, M.C., Biochemical markers of oxidative stress in Mugil cephalus exposed to cadmium, copper, lead and zinc (2011) Int. J. Pharma Biol. Sci., 2 (3), pp. 41-50
  • Raspor, B., Dragun, Z., Erk, M., Examining the suitability of mussel digestive gland to serve as a biomonitoring target organ (2005) Arh. Hig. Rada. Toksikol., 53, pp. 141-149
  • Regoli, F., Trace metals and antioxidant enzymes in gills and digestive gland of the Mediterranean mussel Mytilus galloprovincialis (1998) Arch. Environ. Contam. Toxicol., 34, pp. 48-63
  • Regoli, F., Total oxyradical scavenging capacity (TOSC) in polluted and translocated mussels: a predictive biomarker of oxidative stress (2000) Aquat. Toxicol., 50, pp. 351-361
  • Regoli, F., Winston, G.W., Quantification of total oxidant scavenging capacity TOSC of antioxidants for peroxynitrite, peroxyl radicals and hydroxyl radicals (1999) Toxicol. Appl. Pharmacol., 156, pp. 96-105
  • Rouane-Hacene, O., Boutiba, Z., Belhaouaria, B., Guibbolini-Sabatier, M.E., Francour, P., Risso-de Faverney, C., Seasonal assessment of biological indices, bioaccumulation and bioavailability of heavy metals in mussels Mytilus galloprovincialis from Algerian west coast, applied to environmental monitoring (2015) Oceanologia, 57 (4), pp. 362-374
  • Sabatini, S.E., Rocchetta, I., Nahabedian, D.E., Luquet, C.M., Eppis, M.R., Bianchi, L., Ríos de Molina, M.C., Oxidative stress and histological alterations produced by dietary copper in the fresh water bivalve Diplodon chilensis (2011) Comp. Biochem. Physiol., 154, pp. 391-398
  • Sheehan, D., McDonagh, B., Oxidative stress and bivalves: a proteomic approach (2008) Invertebr. Surviv. J., 5, pp. 110-123
  • Sokal, R.R., Rohlf, F.J., (1999), Introducción a la Bioestadística, Reverté Barcelona, Spain; Strižak, Z., Ivanković, D., Pröfrock, D., Helmholz, H., Cindrić, A.M., Erk, M., Prange, A., Characterization of the cytosolic distribution of priority pollutant metals and metalloids in the digestive gland cytosol of marine mussels: seasonal and spatial variability (2014) Sci. Total Environ., 470-471, pp. 159-170
  • Taylor, A.M., Edge, K.J., Ubrihien, R.P., Maher, W.A., The freshwater bivalve Corbicula australis as a sentinel species for metal toxicity assessment: an in situ case study integrating chemical and biomarker analyses (2016) Environ. Toxicol. Chem., 36 (3), pp. 709-719
  • Torres, M.A., Testa, C.P., Gáspari, C., Masutti, M.B., Panitz, C.M.N., Curi-Pedrosa, R., Almeida, E.A., Filho, D.W., Oxidative stress in the mussel Mytella guyanensis from polluted mangroves on Santa Catarina Island, Brazil (2002) Mar. Pollut. Bull., 44, pp. 923-932
  • Troschinski, S., Dieterich, A., Krais, S., Triebskorn, R., Köhler, H.R., Antioxidant defence and stress protein induction following heat stress in the Mediterranean snail Xeropicta derbentina (2014) J. Exp. Biol., 217, pp. 4399-4405
  • Tsangaris, C., Kormas, K., Strogyloudi, E., Hatzianestis, I., Neofitou, C., Andral, B., Galgani, F., Multiple biomarkers of pollution effects in caged mussels on the Greek coastline (2010) Comp. Biochem. Physiol. C., 151 (3), pp. 369-378
  • Türkmen, M., Ciminli, C., Determination of metals in fish and mussel species by inductively coupled plasma-atomic emission spectrometry (2007) Food Chem., 103 (2), pp. 670-675
  • Valavanidis, A., Vlahogianni, T., Dassenakis, M., Scoullos, M., Molecular biomarkers of oxidative stress in aquatic organisms in relation to toxic environmental pollutants (2006) Ecotoxicol. Environ. Saf., 64, pp. 178-189
  • van der Oost, R., Beyer, J., Vermeulen, N.P.E., Fish bioaccumulation and biomarkers in environmental risk assessment: a review (2003) Environ. Toxicol. Pharmacol., 13, pp. 57-149
  • Viarengo, A., Canesi, L., Pertica, M., Poli, G., Moore, M.N., Orunesu, M., Heavy metals effects on lipid peroxidation in the tissues of Mytilus galloprovincialis (1990) Comp. Biochem. Physiol. C, 97, pp. 37-42
  • Vlahogianni, T., Dassenakis, M., Scoullos, M.J., Valavanidis, A., Integrated use of biomarkers (superoxide dismutase, catalase and lipid peroxidation) in mussels Mytilus galloprovincialis for assessing heavy metals’ pollution in coastal areas from the Saronikos Gulf of Greece (2007) Mar. Poll. Bull., 54, pp. 1361-1371
  • Wang, W.X., Fisher, N.S., Assimilation of trace elements and carbon by the mussel Mytilus edulis: effects of food composition (1996) Limnol. Oceanogr., 41 (2), pp. 197-207
  • Winterbourn, C.C., Hampton, M.B., Thiol chemistry and specificity in redox signaling (2008) Free Radic. Biol. Med., 45 (5), pp. 549-561
  • Zaixso, H.E., Distribución submareal del mitílido Aulacomya atra (Molina) em el Golfo San José (Argentina) en relación a la profundidad, características del fondo y condiciones hidrográficas (1999) Physis (Buenos Aires), A57, pp. 1-10
  • Zhao, H., Li, W., Zhao, X., Li, X., Yang, D., Ren, H., Zhou, Y., Cu/Zn superoxide dismutase (SOD) and catalase (CAT) response to crude oil exposure in the polychaete Perinereis aibuhitensis (2017) Environ. Sci. Pollut. Res. Int., 24 (1), pp. 616-627

Citas:

---------- APA ----------
Ruiz, M.D., Iriel, A., Yusseppone, M.S., Ortiz, N., Di Salvatore, P., Fernández Cirelli, A., Ríos de Molina, M.C.,..., Sabatini, S.E. (2018) . Trace metals and oxidative status in soft tissues of caged mussels (Aulacomya atra) on the North Patagonian coastline. Ecotoxicology and Environmental Safety, 155, 152-161.
http://dx.doi.org/10.1016/j.ecoenv.2018.02.064
---------- CHICAGO ----------
Ruiz, M.D., Iriel, A., Yusseppone, M.S., Ortiz, N., Di Salvatore, P., Fernández Cirelli, A., et al. "Trace metals and oxidative status in soft tissues of caged mussels (Aulacomya atra) on the North Patagonian coastline" . Ecotoxicology and Environmental Safety 155 (2018) : 152-161.
http://dx.doi.org/10.1016/j.ecoenv.2018.02.064
---------- MLA ----------
Ruiz, M.D., Iriel, A., Yusseppone, M.S., Ortiz, N., Di Salvatore, P., Fernández Cirelli, A., et al. "Trace metals and oxidative status in soft tissues of caged mussels (Aulacomya atra) on the North Patagonian coastline" . Ecotoxicology and Environmental Safety, vol. 155, 2018, pp. 152-161.
http://dx.doi.org/10.1016/j.ecoenv.2018.02.064
---------- VANCOUVER ----------
Ruiz, M.D., Iriel, A., Yusseppone, M.S., Ortiz, N., Di Salvatore, P., Fernández Cirelli, A., et al. Trace metals and oxidative status in soft tissues of caged mussels (Aulacomya atra) on the North Patagonian coastline. Ecotoxicol. Environ. Saf. 2018;155:152-161.
http://dx.doi.org/10.1016/j.ecoenv.2018.02.064