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Abstract:

Tracheobronchial airways from Otaria flavescens routinely were processed for histological, histochemical, and immunohistochemical methods to study individual mucus-producing cells (IMPC and submucosal glands. The IMPCs were interspersed in the epithelium of the trachea. They showed neutral and weakly acidic and carboxylated glycoconjugates. Only IMPCs in the trachea secrete sialylated glycoconjugates. Submucosal glands were formed by 1) collecting duct lined by a ciliated pseudostratified to simple columnar epithelium without cilias. They showed neutral and carboxylic glycoconjugates. 2) Glandular sinus, a branched secretory unit in which the lining epithelium showed two possible secretory mechanisms: merocrine type of neutral glycoconjugates and apocrine type of sialylated and sulphated or carboxylated glycoconjugates, with positive reaction for proteins. 3) Glandular branched tubules emerging from glandular sinuses were lined by columnar cells with an apical cytoplasm containing neutral and carboxylated glycoconjugates. Myoepithelial cells surrounded both glandular sinuses and tubules. Cytokeratin 7 was specific for glandular sinus and for a few ciliated cells of the trachea, whereas cytokeratin 19 was observed in glandular sinuses, glandular tubules, and in some tracheal cells. These results showed that the secretory cells of glandular sinuses and tubular glands are well differentiated and that a possible common origin may be inferred.

Registro:

Documento: Artículo
Título:Morphologic, histochemical, and immunohistochemical characterization of tracheobronchial respiratory glands in South American Sea Lion Otaria flavescens (Pinnipedia, Mammalia)
Autor:Lauria De Cidre, L.S.; Crespo, F.A.; Marcos, H.J.A.
Filiación:Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Department of Pathology and Laboratory Medicine, University of Louisville, Louisville, KY, United States
Laboratorio de Histología, Facultad de Medicina, Universidad de Morón, Machado, Argentina
Departamento de Biodiversidad y Biología Experimental, Universidad de Buenos Aires, Ciudad Universitaria (C1428 EHA), Buenos Aires, Argentina
Palabras clave:Airways; Glandular secretion; Glycoconjugates; Submucosal glands; cytokeratin 19; cytokeratin 7; glycoconjugate; acidity; animal cell; animal tissue; article; carboxylation; columnar epithelium; cytoplasm; histochemistry; histology; immunohistochemistry; mammal; morphology; myoepithelium cell; nonhuman; seal; secretory cell; sialylation; South America; submucosa; trachea mucosa; tracheobronchial tree; Animalia; Mammalia; Otaria byronia; Pinnipedia
Año:2006
Volumen:29
Número:1
Página de inicio:29
Página de fin:35
DOI: http://dx.doi.org/10.1179/his.2006.29.1.29
Título revista:Journal of Histotechnology
Título revista abreviado:J. Histotechnol.
ISSN:01478885
CODEN:JOHID
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01478885_v29_n1_p29_LauriaDeCidre

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Citas:

---------- APA ----------
Lauria De Cidre, L.S., Crespo, F.A. & Marcos, H.J.A. (2006) . Morphologic, histochemical, and immunohistochemical characterization of tracheobronchial respiratory glands in South American Sea Lion Otaria flavescens (Pinnipedia, Mammalia). Journal of Histotechnology, 29(1), 29-35.
http://dx.doi.org/10.1179/his.2006.29.1.29
---------- CHICAGO ----------
Lauria De Cidre, L.S., Crespo, F.A., Marcos, H.J.A. "Morphologic, histochemical, and immunohistochemical characterization of tracheobronchial respiratory glands in South American Sea Lion Otaria flavescens (Pinnipedia, Mammalia)" . Journal of Histotechnology 29, no. 1 (2006) : 29-35.
http://dx.doi.org/10.1179/his.2006.29.1.29
---------- MLA ----------
Lauria De Cidre, L.S., Crespo, F.A., Marcos, H.J.A. "Morphologic, histochemical, and immunohistochemical characterization of tracheobronchial respiratory glands in South American Sea Lion Otaria flavescens (Pinnipedia, Mammalia)" . Journal of Histotechnology, vol. 29, no. 1, 2006, pp. 29-35.
http://dx.doi.org/10.1179/his.2006.29.1.29
---------- VANCOUVER ----------
Lauria De Cidre, L.S., Crespo, F.A., Marcos, H.J.A. Morphologic, histochemical, and immunohistochemical characterization of tracheobronchial respiratory glands in South American Sea Lion Otaria flavescens (Pinnipedia, Mammalia). J. Histotechnol. 2006;29(1):29-35.
http://dx.doi.org/10.1179/his.2006.29.1.29