Artículo

Gómez, E.B.; Santori, M.I.; Laría, S.; Engel, J.C.; Swindle, J.; Eisen, H.; Szankasi, P.; Téllez-Ión, M.T. "Characterization of the Trypanosoma cruzi Cdc2p-related protein kinase 1 and identification of three novel associating cyclins" (2001) Molecular and Biochemical Parasitology. 113(1):97-108
El editor solo permite decargar el artículo en su versión post-print desde el repositorio. Por favor, si usted posee dicha versión, enviela a
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Several Cdc2p-related protein kinases (CRKs) have been described in trypanosomatids but their role in the control of the cell cycle nor their biological functions have been addressed. In Trypanosoma cruzi two CRKs have been identified, TzCRK1 and TzCRK3. In this work we further characterize T. cruzi CRK1 and report the identification of three novel associating cyclins. We demonstrate that CRK1 levels and localization do not vary during the cell cycle, and show that it is localized in the cytoplasm, discrete regions of the nucleus, and is highly concentrated in the mitochondrion DNA (kinetoplast), suggesting a putative control function in this organelle. Using purified anti-CRK1 IgGs, we immunoprecipitated from the soluble fraction of T. cruzi epimastigote forms a protein kinase activity which is not inhibited by CDK inhibitors. In addition, we co-precipitated with p13Suc1p beads a kinase activity that was inhibited by the CDK inhibitor flavopiridol and olomoucine. Lastly, using the yeast two-hybrid system we identified three novel cyclin-like proteins able to associate with TzCRK1, and demonstrate that two of these cyclins also bind the T. cruzi CRK3 protein, indicating that these two CRKs are cyclin-dependent kinases. © 2001 Elsevier Science B.V.

Registro:

Documento: Artículo
Título:Characterization of the Trypanosoma cruzi Cdc2p-related protein kinase 1 and identification of three novel associating cyclins
Autor:Gómez, E.B.; Santori, M.I.; Laría, S.; Engel, J.C.; Swindle, J.; Eisen, H.; Szankasi, P.; Téllez-Ión, M.T.
Filiación:Instituto de Investigaciones en Ingenieria Genetica y Biologia Molecular (INGEBI, CONICET) and Fac. de Cie. Exact. y Nat., Univ. de Buenos Aires (UBA), Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
Department of Pathology, University of California, San Francisco, CA 94121, United States
Seattle Biomedical Research Institute, 4 Nickerson Street, Seattle, WA 98109, United States
Fred Hutchinson Cancer Research Institute, 1100 Fairview Avenue, Seattle, WA 98102, United States
Palabras clave:Cdc2p-related kinases; Cell cycle; Cyclins; Kinetoplast; Trypanosoma cruzi; Two-hybrid system; cyclin dependent kinase; cyclin dependent kinase inhibitor; cycline; flavopiridol; mitochondrial DNA; olomoucine; protein kinase; protein kinase inhibitor; article; cell cycle; cell organelle; cellular distribution; controlled study; enzyme activity; enzyme inhibition; epimastigote; immunoprecipitation; nonhuman; priority journal; protein analysis; protein localization; protein protein interaction; Trypanosoma cruzi; Amino Acid Sequence; Animals; CDC2 Protein Kinase; CDC2-CDC28 Kinases; Cyclin-Dependent Kinases; Cyclins; Cytoplasm; Enzyme Inhibitors; Flavonoids; Histones; Immunoglobulin G; Immunohistochemistry; Kinetin; Mitochondria; Molecular Sequence Data; Piperidines; Precipitin Tests; Protein Kinases; Protozoan Proteins; Purines; Retinoblastoma Protein; Sequence Alignment; Trypanosoma cruzi; Trypanosoma; Trypanosoma cruzi; Trypanosomatidae
Año:2001
Volumen:113
Número:1
Página de inicio:97
Página de fin:108
DOI: http://dx.doi.org/10.1016/S0166-6851(00)00382-0
Título revista:Molecular and Biochemical Parasitology
Título revista abreviado:Mol. Biochem. Parasitol.
ISSN:01666851
CODEN:MBIPD
CAS:CDC2 Protein Kinase, EC 2.7.1.37; CDC2-CDC28 Kinases, EC 2.7.1.37; CRK3 kinase, protozoa, EC 2.7.10.-; Cyclin-Dependent Kinases, EC 2.7.1.37; Cyclins; Enzyme Inhibitors; Flavonoids; flavopiridol, 146426-40-6; Histones; Immunoglobulin G; Kinetin, 525-79-1; olomoucine; Piperidines; Protein Kinases, EC 2.7.1.37; Protozoan Proteins; Purines; Retinoblastoma Protein
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v113_n1_p97_Gomez

Referencias:

  • Morgan, D., Principles of CDK regulation (1995) Nature, 374, pp. 131-134
  • Andrews, B., Measday, V., The cyclin family of budding yeast: Abundant use of a good idea (1998) Trends Genet, 14, pp. 66-72
  • Gómez, E., Kornblihtt, A.R., Téllez-Inón, M.T., Cloning of a cdc2-related protein kinase from the parasite Trypanosoma cruzi that interacts with mammalian cyclins (1998) Mol Biol Parasitol, 91, pp. 337-351
  • De Souza, W., Cell biology of Trypanosoma cruzi (1984) Int Rev Cytol, 86, pp. 197-283
  • Mottram, J.C., Kinnaird, J.H., Shiels, B., Tait, A., Barry, D., A novel cdc2-related protein kinase from Leishmania mexicana LmmCRK1, is post-translationally regulated during the life cycle (1993) J Biol Chem, 268, pp. 21044-21052
  • Mottram, J.C., Smith, G., A family of trypanosome cdc2-related protein kinases (1995) Gene, 162, pp. 147-152
  • Mottram, J., McCready, P., Brown, K., Grant, K., Gene disruption indicate an essential function for the LmmCRK1 cdc2-related kinase of Leishmania mexicana (1996) Mol Microbiol, 22, pp. 573-582
  • Grant, K.M., Hassan, P., Anderson, J.S., Mottram, J.C., The crk3 gene of Leishmania mexicana encodes a stage-regulated cdc2-related histone H1 kinase that associates with p12cks1 (1998) J Biol Chem, 24, pp. 10153-10159
  • Wang, Y., Dimitrov, K., Garrity, L., Sazer, S., Beverley, S., Stage-specific activity of the Leishmania major CRK3 kinase and functional rescue of a Schizosaccharomyces pombe cdc2 mutant (1998) Mol Biochem Parasitol, 96, pp. 139-150
  • Brown, L., Hines, J.C., Ray, D.S., The Crithidia fasciculata CRK gene encodes a novel CDC2-related protein containing large inserts between highly conserved domains (1992) Nucleic Acids Res, 20, pp. 5452-5456
  • Affranchino, J.L., González, S.A., Pays, E., Isolation of a mitotic-like cyclin homologue from the protozoan Trypanosoma brucei (1993) Gene, 132, pp. 75-82
  • Van Hellemond, J.J., Neuville, P., Schwartz, R.J., Matthews, K.R., Mottram, J.C., Isolation of Trypanosoma brucei CYC2 and CYC3 cyclin genes by rescue of a yeast G1 cyclin mutant. Functional characterization of CYC2 (2000) J Biol Chem, 275, pp. 8315-8323
  • Engel, J.C., Parodi, A.J., Trypanosoma cruzi cells undergo an alteration in protein N-glycosylation upon differentiation (1985) J Biol Chem, 260, pp. 10105-10110
  • Engel, J.C., Franke de Cazzulo, B.M., Stoppani, A.O.M., Cannata, J.J.B., Cazzulo, J.J., Aerobic glucose fermentation by Trypanosoma cruzi axenic culture amastigote-like forms during growth and differentiation to epimastigotes (1987) Mol Biochem Parasitol, 26, pp. 1-11
  • Engel, J.C., Dvorak, J.A., Trypanosoma cruzi: Cell biological behavior of epimastigote and amastigote forms in axenic culture (1988) J Protozool, 35, pp. 513-518
  • Gómez, M.L., Erijman, L., Araujo, S., Torres, H.N., Téllez-Inón, M.T., Protein kinase C in Trypanosoma cruzi epimastigote forms: Partial purification and characterization (1989) Mol Biochem Parasitol, 36, pp. 101-108
  • Hollenberg, S., Sternglanz, R., Cheng, P.G., Weintraub, H., Identification of a new family of tissue-specific basic helix-loop-helix proteins with the two-hybrid system (1995) Mol Cell Biol, 15, pp. 3813-3822
  • Kjeldsen, L., Bainton, D.F., Sengelov, H., Borregaard, N., Structural and functional heterogeneity among peroxidase-negative granules in human neutrophil: Identification of a distinct gelatinase-containing granule subset by combined immuno cytochemistry and subcellular fractionation (1993) Blood, 82, pp. 3183-3191
  • Engel, J.C., Doyle, P.S., Palmer, J., Hsieh, I., Bainton, D.F., McKerrow, J.H., Growth arrest of T. cruzi by cysteine protease inhibitors is accompanied by alterations in Golgi complex and ER ultrastructure (1998) J Cell Sci, 111, pp. 597-606
  • Harlow, E., Lane, D., Storing and purifying antibodies (1988) Antibodies: a laboratory manual, 2, pp. 284-318. , New York: Cold Spring Harbor
  • Meyerson, M., Harlow, E., Identification of a G1 kinase activity for cdk6, a novel cyclin D partner (1994) Mol Cell Biol, 14, pp. 2077-2086
  • Chien, C.T., Bartel, P.L., Sternglanz, R., Fields, S., The two-hybrid system: A method to identify and clone genes for proteins that interact with a protein of interest (1993) Proc Natl Acad Sci USA, 88, pp. 9578-9582
  • Bartel, P.L., Chien, C.T., Sternglanz, R., Fields, S., Using the two-hybrid system to detect protein-protein interactions (1993), pp. 153-179. , Hartley DA, editor. Cellular interactions in development: a practical approach. Oxford: IRL Press; Altschul, S., Madden, T., Schäffer, A., Zhang, J., Zhang, Z., Miller, W., Lipman, D., Gapped BLAST and PSI-BLAST: A new generation of protein database search programs (1997) Nucleic Acids Res, 25, pp. 3389-3402
  • Boothroyd, J.C., Cross, G.A.M., Transcripts coding for variant surface glycoproteins of Trypanosoma brucei have a short, identical exon at their 5′ end (1982) Gene, 20, pp. 281-289
  • Meijer, L., Chemical inhibitors of cyclin-dependent kinases (1996) Trends Cell Biol, 3, pp. 393-397
  • Pines, J., Cell cycle: Reaching for a role for the Cks proteins (1995) Curr Biol, 6, pp. 1399-1402
  • Brizuela, L., Draetta, G., Beach, D., p13Suc1p acts in the fission yeast cell division cycle as a component of the p34cdc2 protein kinase (1987) EMBO J, 6, pp. 3507-3514
  • Dunphy, W.G., Brizuela, L., Beach, D., Newport, J., The Xenopus cdc2 protein is a component of MPF, a cytoplasmic regulator of mitosis (1988) Cell, 54, pp. 423-431
  • Draetta, G., Beach, D., Activation of cdc2 protein kinase during mitosis in human cells: Cell cycle-dependent phosphorylation and subunit rearrangement (1988) Cell, 54, pp. 17-26
  • Woodward, R., Gull, K., Timing of nuclear and kinetoplast DNA replication and early morphological events in the cell cycle of Trypanosoma brucei (1990) J Cell Sci, 95, pp. 49-57
  • Mottram, J.C., cdc2-related protein kinases and cell cycle control in trypanosomatids (1994) Parasitol Today, 10, pp. 253-257
  • Pasion, S.G., Hines, J.C., Aebersold, R., Ray, D.S., Molecular cloning and expression of the gene encoding the kinetoplast-associated type II DNA topoisomerase of Crithidia fasciculata (1992) Mol Biochem Parasitol, 50, pp. 57-67
  • Priest, J.W., Wood, Z.A., Hajduk, S.L., Cytochromes c1 of kinetoplastid protozoa lack mitochondrial targeting presequences (1993) Biochim Biophys Acta, 1144, pp. 229-231
  • Kang, S., Metzenberg, R.L., Insertional mutagenesis in Neurospora crassa: Cloning and molecular analysis of the preg + gene controlling the activity of the transcriptional activator NUC-1 (1993) Genetics, 133, pp. 193-202
  • Kaffman, A., Herkowitz, I., Tjian, R., O'Shea, E., Phosphorylation of the transcription factor PHO4 by a cyclin-CDK complex, PHO80-PHO85 (1994) Science, 263, pp. 1153-1156
  • Lee, J., Colwill, K., Aneliunas, V., Tennyson, C., Moore, L., Ho, Y., Andrews, B., Interaction of yeast Rvs167 and Pho85 cyclin-dependent kinase complexes may link the cell cycle to the actin cytoskeleton (1998) Curr Biol, 8, pp. 1310-1321

Citas:

---------- APA ----------
Gómez, E.B., Santori, M.I., Laría, S., Engel, J.C., Swindle, J., Eisen, H., Szankasi, P.,..., Téllez-Ión, M.T. (2001) . Characterization of the Trypanosoma cruzi Cdc2p-related protein kinase 1 and identification of three novel associating cyclins. Molecular and Biochemical Parasitology, 113(1), 97-108.
http://dx.doi.org/10.1016/S0166-6851(00)00382-0
---------- CHICAGO ----------
Gómez, E.B., Santori, M.I., Laría, S., Engel, J.C., Swindle, J., Eisen, H., et al. "Characterization of the Trypanosoma cruzi Cdc2p-related protein kinase 1 and identification of three novel associating cyclins" . Molecular and Biochemical Parasitology 113, no. 1 (2001) : 97-108.
http://dx.doi.org/10.1016/S0166-6851(00)00382-0
---------- MLA ----------
Gómez, E.B., Santori, M.I., Laría, S., Engel, J.C., Swindle, J., Eisen, H., et al. "Characterization of the Trypanosoma cruzi Cdc2p-related protein kinase 1 and identification of three novel associating cyclins" . Molecular and Biochemical Parasitology, vol. 113, no. 1, 2001, pp. 97-108.
http://dx.doi.org/10.1016/S0166-6851(00)00382-0
---------- VANCOUVER ----------
Gómez, E.B., Santori, M.I., Laría, S., Engel, J.C., Swindle, J., Eisen, H., et al. Characterization of the Trypanosoma cruzi Cdc2p-related protein kinase 1 and identification of three novel associating cyclins. Mol. Biochem. Parasitol. 2001;113(1):97-108.
http://dx.doi.org/10.1016/S0166-6851(00)00382-0