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Abstract:

Two cdc2-related protein kinases (crk), tzcrk3 and tzcrk1, from the protozoan parasite Trypanosoma cruzi were cloned. tzcrk3 encodes a 35 kDa protein sharing 51.5% amino acid identity with human cdc2 and 82% identity with Trypanosoma brucei CRK3. tzcrk1 encodes a 33 kDa protein sharing 52.7% identity with human cdc2 and a high degree of identity (>78%) with T. brucei CRK1, Leishmania mexicana CRK1 and Trypanosoma congolense CRK1. A recombinant TzCRK1 protein was able to phosphorylate histone HI and retinoblastoma protein. Western blotting using a polyclonal antibody raised against the recombinant TzCRK1 protein showed that the kinase is present in all life cycle stages of the parasite. A PSTAIRE antiserum detected proteins of 32, 33 and 35 kDa, with differential expression in the life cycle of the parasite. Transfection of COS-7 cells with tzcrk1 demonstrated for the first time that a CRK protein can bind mammalian cyclins; TzCRK1 co-immunoprecipitated with cyclins E, D3 and A suggesting a role for this kinase in cell cycle control. These results indicate that T. cruzi might have cyclin homologues that control the activity of the CRK proteins and that a complex mechanism would exist in order to regulate the kinases involved in the cell cycle and the differentiation processes of the parasite. Copyright (C) 1998 Elsevier Science B.V.

Registro:

Documento: Artículo
Título:Cloning of a cdc2-related protein kinase from Trypanosoma cruzi that interacts with mammalian cyclins
Autor:Gómez, E.B.; Kornblihtt, A.R.; Téllez-Iñón, M.T.
Filiación:Inst. de Invest. en Ing. Genet. y B., Fac. de Ciencias Exactas y Naturales, University of Buenos Aires (UBA), Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:cdc2-related protein kinase; Cell cycle control; Mammalian cyclins; Trypanosoma cruzi; cycline; protein kinase; amino acid sequence; article; cell cycle; enzyme analysis; gene sequence; immunoblotting; molecular cloning; nonhuman; nucleotide sequence; priority journal; protein analysis; trypanosoma cruzi; Amino Acid Sequence; Animals; Base Sequence; Blotting, Southern; CDC2 Protein Kinase; Cloning, Molecular; COS Cells; Cyclins; Genes, Protozoan; Genetic Complementation Test; Histones; Humans; Molecular Probe Techniques; Molecular Sequence Data; Protein Kinases; Protozoan Proteins; Recombinant Proteins; Retinoblastoma Protein; Sequence Alignment; Transfection; Trypanosoma cruzi; Leishmania mexicana; Mammalia; Protozoa; Trypanosoma; Trypanosoma brucei; Trypanosoma congolense; Trypanosoma cruzi
Año:1998
Volumen:91
Número:2
Página de inicio:337
Página de fin:351
DOI: http://dx.doi.org/10.1016/S0166-6851(97)00218-1
Título revista:Molecular and Biochemical Parasitology
Título revista abreviado:Mol. Biochem. Parasitol.
ISSN:01666851
CODEN:MBIPD
CAS:CDC2 Protein Kinase, EC 2.7.1.37; CRK protein, protozoan; Cyclins; Histones; Protein Kinases, EC 2.7.1.37; Protozoan Proteins; Recombinant Proteins; Retinoblastoma Protein; tcrk1 protein, Trypanosoma cruzi, EC 2.7.1.-; TCRK3 protein, Trypanosoma cruzi, EC 2.7.1.-
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v91_n2_p337_Gomez

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Citas:

---------- APA ----------
Gómez, E.B., Kornblihtt, A.R. & Téllez-Iñón, M.T. (1998) . Cloning of a cdc2-related protein kinase from Trypanosoma cruzi that interacts with mammalian cyclins. Molecular and Biochemical Parasitology, 91(2), 337 -351.
http://dx.doi.org/10.1016/S0166-6851(97)00218-1
---------- CHICAGO ----------
Gómez, E.B., Kornblihtt, A.R., Téllez-Iñón, M.T. "Cloning of a cdc2-related protein kinase from Trypanosoma cruzi that interacts with mammalian cyclins" . Molecular and Biochemical Parasitology 91, no. 2 (1998) : 337 -351.
http://dx.doi.org/10.1016/S0166-6851(97)00218-1
---------- MLA ----------
Gómez, E.B., Kornblihtt, A.R., Téllez-Iñón, M.T. "Cloning of a cdc2-related protein kinase from Trypanosoma cruzi that interacts with mammalian cyclins" . Molecular and Biochemical Parasitology, vol. 91, no. 2, 1998, pp. 337 -351.
http://dx.doi.org/10.1016/S0166-6851(97)00218-1
---------- VANCOUVER ----------
Gómez, E.B., Kornblihtt, A.R., Téllez-Iñón, M.T. Cloning of a cdc2-related protein kinase from Trypanosoma cruzi that interacts with mammalian cyclins. Mol. Biochem. Parasitol. 1998;91(2):337 -351.
http://dx.doi.org/10.1016/S0166-6851(97)00218-1