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Abstract:

The placenta produces a wide number of molecules that play essential roles in the establishment and maintenance of pregnancy. In this context, leptin has emerged as an important player in reproduction. The synthesis of leptin in normal trophoblastic cells is regulated by different endogenous biochemical agents, but the regulation of placental leptin expression is still poorly understood. We have previously reported that 17β-estradiol (E 2) up-regulates placental leptin expression. To improve the understanding of estrogen receptor mechanisms in regulating leptin gene expression, in the current study we examined the effect of membrane-constrained E 2 conjugate, E-BSA, on leptin expression in human placental cells. We have found that leptin expression was induced by E-BSA both in BeWo cells and human placental explants, suggesting that E 2 also exerts its effects through membrane receptors. Moreover E-BSA rapidly activated different MAPKs and AKT pathways, and these pathways were involved in E 2 induced placental leptin expression. On the other hand we demonstrated the presence of ERα associated to the plasma membrane of BeWo cells. We showed that E 2 genomic and nongenomic actions could be mediated by ERα. Supporting this idea, the downregulation of ERα level through a specific siRNA, decreased E-BSA effects on leptin expression. Taken together, these results provide new evidence of the mechanisms whereby E 2 regulates leptin expression in placenta and support the importance of leptin in placental physiology. © 2012 Elsevier B.V..

Registro:

Documento: Artículo
Título:Regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alpha
Autor:Gambino, Y.P.; Pérez Pérez, A.; Dueñas, J.L.; Calvo, J.C.; Sánchez-Margalet, V.; Varone, C.L.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón 2, 4 piso, (1428), Buenos Aires, Argentina
Departamento de Bioquímica Médica y Biología Molecular, Hospital Universitario Virgen Macarena, Facultad de Medicina, Universidad de Sevilla, Avenida Sánchez Pizjuán 4, (41009) Sevilla, Spain
Servicio de Ginecología y Obstetricia, Hospital Universitario Virgen Macarena, Sevilla, Spain
Instituto de Biología y Medicina Experimental (IBYME), Vuelta de Obligado 2490, (1428), Buenos Aires, Argentina
Palabras clave:17β-estradiol; Estrogen receptor; Gene expression; Leptin; Placenta; estradiol; estrogen receptor alpha; leptin; mitogen activated protein kinase; protein kinase B; article; controlled study; enzyme activation; hormonal regulation; hormone action; hormone synthesis; human; human cell; placenta; priority journal; Cell Membrane; Cell Nucleus; Estradiol; Estrogen Receptor alpha; Female; Gene Expression Regulation; Gene Silencing; Humans; Leptin; Models, Biological; Placenta; Pregnancy; Protein Binding; Protein Transport; Serum Albumin, Bovine; Signal Transduction
Año:2012
Volumen:1823
Número:4
Página de inicio:900
Página de fin:910
DOI: http://dx.doi.org/10.1016/j.bbamcr.2012.01.015
Título revista:Biochimica et Biophysica Acta - Molecular Cell Research
Título revista abreviado:Biochim. Biophys. Acta Mol. Cell Res.
ISSN:01674889
CODEN:BAMRD
CAS:estradiol, 50-28-2; mitogen activated protein kinase, 142243-02-5; protein kinase B, 148640-14-6; Estradiol, 50-28-2; Estrogen Receptor alpha; Leptin; Serum Albumin, Bovine; estrogen receptor alpha, human; fulvestrant, 129453-61-8
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_01674889_v1823_n4_p900_Gambino.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01674889_v1823_n4_p900_Gambino

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Citas:

---------- APA ----------
Gambino, Y.P., Pérez Pérez, A., Dueñas, J.L., Calvo, J.C., Sánchez-Margalet, V. & Varone, C.L. (2012) . Regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alpha. Biochimica et Biophysica Acta - Molecular Cell Research, 1823(4), 900-910.
http://dx.doi.org/10.1016/j.bbamcr.2012.01.015
---------- CHICAGO ----------
Gambino, Y.P., Pérez Pérez, A., Dueñas, J.L., Calvo, J.C., Sánchez-Margalet, V., Varone, C.L. "Regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alpha" . Biochimica et Biophysica Acta - Molecular Cell Research 1823, no. 4 (2012) : 900-910.
http://dx.doi.org/10.1016/j.bbamcr.2012.01.015
---------- MLA ----------
Gambino, Y.P., Pérez Pérez, A., Dueñas, J.L., Calvo, J.C., Sánchez-Margalet, V., Varone, C.L. "Regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alpha" . Biochimica et Biophysica Acta - Molecular Cell Research, vol. 1823, no. 4, 2012, pp. 900-910.
http://dx.doi.org/10.1016/j.bbamcr.2012.01.015
---------- VANCOUVER ----------
Gambino, Y.P., Pérez Pérez, A., Dueñas, J.L., Calvo, J.C., Sánchez-Margalet, V., Varone, C.L. Regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alpha. Biochim. Biophys. Acta Mol. Cell Res. 2012;1823(4):900-910.
http://dx.doi.org/10.1016/j.bbamcr.2012.01.015