Artículo

Fuchs, J.; Piola, L.; González, E.P.; Oneto, M.L.; Basack, S.; Kesten, E.; Casabé, N. "Coelomocyte biomarkers in the earthworm Eisenia fetida exposed to 2,4,6-trinitrotoluene (TNT)" (2011) Environmental Monitoring and Assessment. 175(1-4):127-137
Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Contamination by 2,4,6-trinitrotoluene (TNT) is a global environmental problem at sites of former explosive production, handling, or storage, and could have deleterious consequences for human and ecological health. We investigated its sublethal effects to Eisenia fetida, using two nonspecific biomarkers. In coelomocytes of earthworms exposed 24, 48, or 72 h, we evaluated DNA damage (comet assay) and neutral red retention time (NRRT), using the filter paper contact test. Both percentage of damage (D%) and calculated damage index showed significant DNA damage at almost all concentrations, at all time points assayed. Along exposure time, two different patterns were observed. At the lower TNT concentrations (0.25-0.5 μg/cm 2 ) an increased DNA migration at 48 h, with a decrease close to initial levels after 72 h exposure, was observed. This decrease could be attributed to activation of the DNA repair system. At higher concentrations (1.0-2.0 μg/cm 2 ), the high DNA damage observed remained constant during the 72 h exposure, suggesting that the rate of DNA repair was not enough to compensate such damage. Analysis of NRRT results showed a significant interaction between time and treatment. After 48 h, a significant decrease was observed at 4.0 μg/cm 2 . After 72 h, NRRT presented a concentration-dependent decrease, significantly different with respect to control at 0.5, 1.0, 2.0, and 4.0 μg/cm 2 . The two assayed methods, performed on the same sample, showed clear responses to sublethal TNT exposure in E. fetida, providing sensitive unspecific biomarkers of cell injury and DNA damage. © 2010 Springer Science+Business Media B.V.

Registro:

Documento: Artículo
Título:Coelomocyte biomarkers in the earthworm Eisenia fetida exposed to 2,4,6-trinitrotoluene (TNT)
Autor:Fuchs, J.; Piola, L.; González, E.P.; Oneto, M.L.; Basack, S.; Kesten, E.; Casabé, N.
Filiación:Department of Biological Chemistry, Faculty of Exact and Natural Sciences, University of Buenos Aires, Pabellón 2, 4o piso, Buenos Aires 1428, Argentina
CONICET, Rivadavia 1917, Buenos Aires 1033, Argentina
Centre for Advanced Studies in Health Sciences, UAI -Av. Montes de Oca 745, Buenos Aires 1270, Argentina
Palabras clave:2,4,6-trinitrotoluene (TNT); Biomarkers; Coelomocytes; Comet assay; Eisenia fetida; Neutral red retention time; 2,4,6-trinitrotoluene (TNT); Coelomocytes; Comet assay; Eisenia fetida; Neutral red retention time; Concentration (process); Damage detection; Nucleic acids; DNA; trinitrotoluene; biomarker; concentration (composition); DNA; earthworm; sublethal effect; trinitrotoluene; analytic method; article; calculation; cell type; cell viability; celomocyte; comet assay; contamination; controlled study; DNA damage; DNA repair; earthworm; environmental exposure; environmental impact assessment; natural red retention time; nonhuman; Animals; Biological Markers; Comet Assay; Environmental Monitoring; Oligochaeta; Trinitrotoluene; Eisenia fetida
Año:2011
Volumen:175
Número:1-4
Página de inicio:127
Página de fin:137
DOI: http://dx.doi.org/10.1007/s10661-010-1499-z
Título revista:Environmental Monitoring and Assessment
Título revista abreviado:Environ. Monit. Assess.
ISSN:01676369
CODEN:EMASD
CAS:trinitrotoluene, 118-96-7; Biological Markers; Trinitrotoluene, 118-96-7
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01676369_v175_n1-4_p127_Fuchs

Referencias:

  • Achtnich, C., Lenke, H., Stability of immobilized 2,4,6-trinitrotoluene metabolites in soil under long-term leaching conditions (2001) Environmental Toxicology and Chemistry, 20 (2), pp. 280-283
  • Ateeq, B., Farah, M.A., Ahmad, W., Detection of DNA damage by alkaline single cell gel electrophoresis in 2,4-dichlorophenoxyacetic-acid- and butachlor-exposed erythrocytes of Clarias batrachus (2005) Ecotoxicology and Environmental Safety, 62 (3), pp. 348-354. , DOI 10.1016/j.ecoenv.2004.12.011, PII S0147651304002556
  • Batzias, F.A., Siontorou, C.G., A knowledge-based approach to environmental biomonitoring (2006) Environmental Monitoring and Assessment, 123 (1-3), pp. 167-197. , DOI 10.1007/s10661-006-9190-0
  • Belden, J.B., Ownby, D.R., Lotufo, G.R., Lydy, M.J., Accumulation of trinitrotoluene (TNT) in aquatic organisms: Part 2-Bioconcentration in aquatic invertebrates and potential for trophic transfer to channel catfish (Ictalurus punctatus) (2005) Chemosphere, 58, pp. 1161-1168. , 10.1016/j.chemosphere.2004.09.058 1:CAS:528:DC%2BD2MXmvFalug%3D%3D
  • Bolt, H.M., Degen, G.H., Dorn, S.B., Plottner, S., Harth, V., Genotoxicity and potential carcinogenicity of 2,4,6-TNT trinitrotoluene: Structural and toxicological considerations (2006) Reviews on Environmental Health, 21 (4), pp. 217-228. , Copper and Inflammation
  • Burch, S.W., Fitzpatrick, L.C., Goven, A.J., Venables, B.J., Giggleman, M.A., In vitro earthworm Lumbricus terrestris coelomocyte assay for use in terrestrial toxicity identification evaluation (1999) Bulletin of Environmental Contamination and Toxicology, 62 (5), pp. 547-554. , DOI 10.1007/s001289900910
  • Burlinson, B., Tice, R.R., Speit, G., Agurell, E., Brendler-Schwaab, S.Y., Collins, A.R., Escobar, P., Hartmann, A., Fourth International Workgroup on Genotoxicity testing: Results of the in vivo Comet assay workgroup (2007) Mutation Research - Genetic Toxicology and Environmental Mutagenesis, 627 (1), pp. 31-35. , DOI 10.1016/j.mrgentox.2006.08.011, PII S1383571806003688, Reports of the 4th International Workshop on Genotoxicity Testing (IWGT), September 9-10, 2005, San Francisco, CA, USA
  • Bursch, W., The autophagosomal-lysosomal compartment in programmed cell death (2001) Cell Death and Differentiation, 8 (6), pp. 569-581. , DOI 10.1038/sj.cdd.4400852
  • Casabé, N.B., Oneto, M.L., Fuchs, J.S., Sánchez-Rivas, C., Kesten, E.M., Nitrocompuestos aromáticos explosivos: Biotransformación, toxicidad y remediación (2003) Acta Bioquímica Clínica Latinoamericana, 37, pp. 47-58
  • Casabe, N., Piola, L., Fuchs, J., Oneto, M.L., Pamparato, L., Basack, S., Gimenez, R., Kesten, E., Ecotoxicological assessment of the effects of glyphosate and chlorpyrifos in an Argentine soya field (2007) Journal of Soils and Sediments, 7 (4), pp. 232-239. , DOI 10.1065/jss2007.04.224
  • Fuchs, J.S., Oneto, M.L., Casabe, N.B., Gomez Segura, O., Tarulla, R., Vaccarezza, M., Sanchez-Rivas, C., Wood, E.J., Ecotoxicological characterization of a disposal lagoon from a munition plant (2001) Bulletin of Environmental Contamination and Toxicology, 67 (5), pp. 696-703. , DOI 10.1007/s001280179
  • Fugere, N., Brousseau, P., Krzystyniak, K., Coderre, D., Fournier, M., Heavy metal-specific inhibition of phagocytosis and different in vitro sensitivity of heterogeneous coelomocytes from Lumbricus terrestris (Oligochaeta) (1996) Toxicology, 109 (2-3), pp. 157-166. , DOI 10.1016/0300-483X(96)03315-X
  • Gastaldi, L., Ranzato, E., Capri, F., Hankard, P., Peres, G., Canesi, L., Viarengo, A., Pons, G., Application of a biomarker battery for the evaluation of the sublethal effects of pollutants in the earthworm Eisenia andrei (2007) Comparative Biochemistry and Physiology - C Toxicology and Pharmacology, 146 (3), pp. 398-405. , DOI 10.1016/j.cbpc.2007.04.014, PII S1532045607001366
  • Gong, P., Guan, X., Inouye, L.S., Pirooznia, M., Indest, K.J., Athow, R.S., Deng, Y., Perkins, E.J., Toxicogenomic analysis provides new insights into molecular mechanisms of the sublethal toxicity of 2,4,6-trinitrotoluene in Eisenia fetida (2007) Environmental Science and Technology, 41 (23), pp. 8195-8202. , DOI 10.1021/es0716352
  • Guecheva, T., Henriques, J.A.P., Erdtmann, B., Genotoxic effects of copper sulphate in freshwater planarian in vivo, studied with the single-cell gel test (comet assay) (2001) Mutation Research - Genetic Toxicology and Environmental Mutagenesis, 497 (1-2), pp. 19-27. , DOI 10.1016/S1383-5718(01)00244-3, PII S1383571801002443
  • Hwang, H.-M., Wade, T.L., Sericano, J.L., Destabilized lysosomes and elimination of polycyclic aromatic hydrocarbons and polychlorinated biphenyls in eastern oysters (Crassostrea virginica) (2004) Environmental Toxicology and Chemistry, 23 (8), pp. 1991-1995. , DOI 10.1897/03-467
  • (2008) Soil Quality-avoidance Test for Testing the Quality of Soils and Effects of Chemicals on Behaviour-Part 1: Test with Earthworms (Eisenia Fetida and Eisenia Andrei), , ISO ISO 17512-1. Geneva: International Organization for Standardization
  • Kumagai, Y., Kikushima, M., Nakai, Y., Shimojo, N., Kunimoto, M., Neuronal nitric oxide synthase (nNOS) catalyzes one-electron reduction of 2,4,6-trinitrotoluene, resulting in decreased nitric oxide production and increased nNOS gene expression: Implication for oxidative stress (2004) Free Radical Biology and Medicine, 37 (3), pp. 350-357. , DOI 10.1016/j.freeradbiomed.2004.04.023, PII S0891584904003442
  • Kumaravel, T., Vilhar, B., Faux, S., Jha, A., Comet assay measurements: A perspective (2009) Cell Biology and Toxicology, 25, pp. 53-64. , 10.1007/s10565-007-9043-9 1:CAS:528:DC%2BD1cXhsFCmsLnJ
  • Lachance, B., Renoux, A.Y., Sarrazin, M., Hawari, J., Sunahara, G.I., Toxicity and bioaccumulation of reduced TNT metabolites in the earthworm Eisenia andrei exposed to amended forest soil (2004) Chemosphere, 55 (10), pp. 1339-1348. , DOI 10.1016/j.chemosphere.2003.11.049, PII S0045653503011925
  • Lee, R.F., Steinert, S., Use of the single cell gel electrophoresis/comet assay for detecting DNA damage in aquatic (marine and freshwater) animals (2003) Mutation Research - Reviews in Mutation Research, 544 (1), pp. 43-64. , DOI 10.1016/S1383-5742(03)00017-6
  • Lewis, T.A., Newcombe, D.A., Crawford, R.L., Bioremediation of soils contaminated with explosives (2004) Journal of Environmental Management, 70 (4), pp. 291-307. , DOI 10.1016/j.jenvman.2003.12.005
  • Maboeta, M.S., Reinecke, S.A., Reinecke, A.J., Linking lysosomal biomarker and population responses in a field population of Aporrectodea caliginosa (Oligochaeta) exposed to the fungicide copper oxychloride (2003) Ecotoxicology and Environmental Safety, 56 (3), pp. 411-418. , DOI 10.1016/S0147-6513(02)00087-8
  • Maleri, R.A., Fourie, F., Reinecke, A.J., Reinecke, S.A., Photometric application of the MTT- and NRR-assays as biomarkers for the evaluation of cytotoxicity ex vivo in Eisenia andrei (2008) Soil Biology & Biochemistry, 40, pp. 1040-1048. , 10.1016/j.soilbio.2007.11.024 1:CAS:528:DC%2BD1cXksVKqsr8%3D
  • Manerikar, R.S., Apte, A.A., Ghole, V.S., In vitro and in vivo genotoxicity assessment of Cr(VI) using comet assay in earthworm coelomocytes (2008) Environmental Toxicology and Pharmacology, 25 (1), pp. 63-68. , DOI 10.1016/j.etap.2007.08.009, PII S1382668907001147
  • Martin-Diaz., M.L., Tuberty, S.R., McKenney Jr., C.L., Blasco, J., Sarasquete, C., DelValls, T.A., The use of bioaccumulation, biomarkers and histopathology diseases in shape Procambarus clarkii to establish bioavailability of Cd and Zn after a mining spill (2006) Environmental Monitoring and Assessment, 116 (1-3), pp. 169-184. , DOI 10.1007/s10661-006-7234-0
  • Moore, M.N., Icarus Allen, J., McVeigh, A., Environmental prognostics: An integrated model supporting lysosomal stress responses as predictive biomarkers of animal health status (2006) Marine Environmental Research, 61 (3), pp. 278-304. , DOI 10.1016/j.marenvres.2005.10.005, PII S0141113605000681
  • Neuwoehner, J., Schofer, A., Erlenkaemper, B., Steinbach, K., Hund-Rinke, K., Eisentraeger, A., Toxicological characterization of 2,4,6-trinitrotoluene, its transformation products, and two nitramine explosives (2007) Environmental Toxicology and Chemistry, 26 (6), pp. 1090-1099. , DOI 10.1897/06-471R.1
  • (1984) Guideline for Testing of Chemicals N°. 207. Earthworm, Acute Toxicity Tests, , OECD Paris: Organization for Economic Cooperation and Development
  • Ownby, D., Belden, J., Lotufo, G., Lydy, M., Accumulation of trinitrotoluene (TNT) in aquatic organisms: Part 1-Bioconcentration and distribution in channel catfish (Ictalurus punctatus) (2005) Chemosphere, 58, pp. 1153-1159. , 10.1016/j.chemosphere.2004.09.059 1:CAS:528:DC%2BD2MXmvFaltQ%3D%3D
  • Qiao, M., Chen, Y., Wang, C.-X., Wang, Z., Zhu, Y.-G., DNA damage and repair process in earthworm after in-vivo and in vitro exposure to soils irrigated by wastewaters (2007) Environmental Pollution, 148 (1), pp. 141-147. , DOI 10.1016/j.envpol.2006.10.033, PII S0269749106005902
  • Reinecke, S.A., Reinecke, A.J., The Comet Assay as Biomarker of Heavy Metal Genotoxicity in Earthworms (2004) Archives of Environmental Contamination and Toxicology, 46 (2), pp. 208-215
  • Renoux, A.Y., Sarrazin, M., Hawari, J., Sunahara, G.I., Transformation of 2,4,6-trinitrotoluene in soil in the presence of the earthworm Eisenia andrei (2000) Environmental Toxicology and Chemistry, 19 (6), pp. 1473-1480
  • Roberts, B.L., Dorough, H.W., Relative toxicities of chemicals to the earthworm Eisenia foetida (1984) Environmental Toxicology and Chemistry, 3 (1), pp. 67-78
  • Robidoux, P.Y., Svendsen, C., Sarrazin, M., Hawari, J., Thiboutot, S., Ampleman, G., Weeks, J.M., Sunahara, G.I., Evaluation of tissue and cellular biomarkers to assess 2,4,6-trinitrotoluene (TNT) exposure in earthworms: Effects-based assessment in laboratory studies using Eisenia andrei (2002) Biomarkers, 7 (4), pp. 306-321. , DOI 10.1080/13547500210127327
  • Scott-Fordsmand, J.J., Weeks, J.M., Biomarkers in earthworms (2000) Reviews of Environmental Contamination and Toxicology, 165, pp. 117-159. , 1:CAS:528:DC%2BD3cXis1Chu7Y%3D
  • Sims, J.G., Steevens, J.A., The role of metabolism in the toxicity of 2,4,6-trinitrotoluene and its degradation products to the aquatic amphipod Hyalella azteca (2008) Ecotoxicology and Environmental Safety, 70 (1), pp. 38-46. , DOI 10.1016/j.ecoenv.2007.08.019, PII S0147651307002059
  • Singh, N.P., McCoy, M.T., Tice, R.R., Schneider, E.L., A simple technique for quantitation of low levels of DNA damage in individual cells (1988) Experimental Cell Research, 175, pp. 123-130. , 10.1016/0014-4827(88)90265-0
  • Sun, L.W., Qu, M.M., Li, Y.Q., Wu, Y.L., Chen, Y.G., Kong, Z.M., Liu, Z.T., Toxic effects of aminophenols on aquatic life using the zebrafish embryo test and the comet assay (2004) Bulletin of Environmental Contamination and Toxicology, 73 (4), pp. 628-634. , DOI 10.1007/s00128-004-0474-1
  • Svendsen, C., Weeks, J.M., Relevance and applicability of a simple earthworm biomarker of copper exposure: I. Links to ecological effects in a laboratory study with Eisenia andrei (1997) Ecotoxicology and Environmental Safety, 36 (1), pp. 72-79. , DOI 10.1006/eesa.1996.1491
  • Svendsen, C., Spurgeon, D.J., Hankard, P.K., Weeks, J.M., A review of lysosomal membrane stability measured by neutral red retention: Is it a workable earthworm biomarker? (2004) Ecotoxicology and Environmental Safety, 57 (1), pp. 20-29. , DOI 10.1016/j.ecoenv.2003.08.009
  • Weeks, J.M., Svendsen, C., Neutral red retention by lysosomes from earthworm (Lumbricus rubellus) coelomocytes: A simple biomarker of exposure to soil copper (1996) Environmental Toxicology and Chemistry, 15 (10), pp. 1801-1805. , DOI 10.1897/1551-5028(1996)015<1801 :NRRBLF>2.3.CO;2
  • Xiao, N., Jing, B., Ge, F., Liu, X., The fate of herbicide acetochlor and its toxicity to Eisenia fetida under laboratory conditions (2006) Chemosphere, 62 (8), pp. 1366-1373. , DOI 10.1016/j.chemosphere.2005.07.043, PII S0045653505009495

Citas:

---------- APA ----------
Fuchs, J., Piola, L., González, E.P., Oneto, M.L., Basack, S., Kesten, E. & Casabé, N. (2011) . Coelomocyte biomarkers in the earthworm Eisenia fetida exposed to 2,4,6-trinitrotoluene (TNT). Environmental Monitoring and Assessment, 175(1-4), 127-137.
http://dx.doi.org/10.1007/s10661-010-1499-z
---------- CHICAGO ----------
Fuchs, J., Piola, L., González, E.P., Oneto, M.L., Basack, S., Kesten, E., et al. "Coelomocyte biomarkers in the earthworm Eisenia fetida exposed to 2,4,6-trinitrotoluene (TNT)" . Environmental Monitoring and Assessment 175, no. 1-4 (2011) : 127-137.
http://dx.doi.org/10.1007/s10661-010-1499-z
---------- MLA ----------
Fuchs, J., Piola, L., González, E.P., Oneto, M.L., Basack, S., Kesten, E., et al. "Coelomocyte biomarkers in the earthworm Eisenia fetida exposed to 2,4,6-trinitrotoluene (TNT)" . Environmental Monitoring and Assessment, vol. 175, no. 1-4, 2011, pp. 127-137.
http://dx.doi.org/10.1007/s10661-010-1499-z
---------- VANCOUVER ----------
Fuchs, J., Piola, L., González, E.P., Oneto, M.L., Basack, S., Kesten, E., et al. Coelomocyte biomarkers in the earthworm Eisenia fetida exposed to 2,4,6-trinitrotoluene (TNT). Environ. Monit. Assess. 2011;175(1-4):127-137.
http://dx.doi.org/10.1007/s10661-010-1499-z