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Abstract:

We detected apoptosis induction in the vesicular stomatitis virus (VSV) infected mammalian cell lines Vero-76, Cos-7, and BHK-21. Cell lines were analyzed by chromosomal DNA fragmentation and nuclear morphology. In order to determine the step in the viral cycle at which apoptosis of infected cells is triggered, chemical and physical agents were used to block viral infection at different times and then the apoptotic response of infected cells was examined. The treatment of Vero-76 infected cells with a lysosomotrophic agent, such as NH4Cl, was shown to abrogate virus apoptosis induction. On the other hand, VSV-induced apoptosis was not blocked by the presence of cycloheximide, suggesting that the de novo viral protein synthesis is not required for this process. UV-inactivated viruses were also capable of inducing apoptosis in Vero-76 cells, indicating that the activation of a programmed cell death process by VSV does not require viral replication. We conclude from these findings that VSV induces apoptosis at early stages of infection. © 2002 Elsevier Science B.V. All rights reserved.

Registro:

Documento: Artículo
Título:Vesicular stomatitis virus induces apoptosis at early stages in the viral cycle and does not depend on virus replication
Autor:Gadaleta, P.; Vacotto, M.; Coulombié, F.
Filiación:Department of Biological Chemistry, Laboratory of Virology, University of Buenos Aires, 1428 Buenos Aires, Argentina
Palabras clave:Apoptosis; Vesicular stomatitis virus; Viral cycle; ammonium chloride; chemical agent; cycloheximide; DNA fragment; virus protein; animal cell; apoptosis; article; cell assay; cell death; cell strain BHK; cell strain COS7; chromosome analysis; controlled study; DNA determination; gene structure; mammal cell; nonhuman; priority journal; protein synthesis; structure analysis; ultraviolet radiation; Vero cell; Vesicular stomatitis virus; virus induction; virus infection; virus replication; animal; biosynthesis; cell culture; Cercopithecus; physiology; radiation exposure; Mammalia; Vesicular stomatitis virus; Animals; Apoptosis; Cercopithecus aethiops; Tumor Cells, Cultured; Ultraviolet Rays; Vero Cells; Vesicular stomatitis-Indiana virus; Viral Proteins; Virus Replication
Año:2002
Volumen:86
Número:1-2
Página de inicio:87
Página de fin:92
DOI: http://dx.doi.org/10.1016/S0168-1702(02)00049-7
Título revista:Virus Research
Título revista abreviado:Virus Res.
ISSN:01681702
CODEN:VIRED
CAS:Viral Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681702_v86_n1-2_p87_Gadaleta

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Citas:

---------- APA ----------
Gadaleta, P., Vacotto, M. & Coulombié, F. (2002) . Vesicular stomatitis virus induces apoptosis at early stages in the viral cycle and does not depend on virus replication. Virus Research, 86(1-2), 87-92.
http://dx.doi.org/10.1016/S0168-1702(02)00049-7
---------- CHICAGO ----------
Gadaleta, P., Vacotto, M., Coulombié, F. "Vesicular stomatitis virus induces apoptosis at early stages in the viral cycle and does not depend on virus replication" . Virus Research 86, no. 1-2 (2002) : 87-92.
http://dx.doi.org/10.1016/S0168-1702(02)00049-7
---------- MLA ----------
Gadaleta, P., Vacotto, M., Coulombié, F. "Vesicular stomatitis virus induces apoptosis at early stages in the viral cycle and does not depend on virus replication" . Virus Research, vol. 86, no. 1-2, 2002, pp. 87-92.
http://dx.doi.org/10.1016/S0168-1702(02)00049-7
---------- VANCOUVER ----------
Gadaleta, P., Vacotto, M., Coulombié, F. Vesicular stomatitis virus induces apoptosis at early stages in the viral cycle and does not depend on virus replication. Virus Res. 2002;86(1-2):87-92.
http://dx.doi.org/10.1016/S0168-1702(02)00049-7