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Abstract:

Red beet (Beta vulgaris L. var. conditiva) root is a popular item present in ready to eat salads and minimally processed foods. In this research, the effect of low doses of gamma radiation (1 and 2 kGy) on peroxidase (POX), polyphenol oxidase (PPO) and phenylalanine ammonia-lyase (PAL) activities, as well as on the levels of compounds related to the response to the oxidative stress of plant metabolism, the changes in colour and the mechanical behaviour of fresh-cut red beet root were analyzed, with the purpose of understanding the influence of the processing on tissue characteristics. Cell wall modifications were also studied through sequential extractions of polysaccharides from the alcohol-insoluble residues (AIR) obtained from each tissue. Irradiation seemed to contribute to higher cell-cell adhesion through increasing of calcium-cross linking at the middle lamellae regions, in addition to an increment of cross-links of polymers into the cell wall. Chemical modifications produced in the cell walls as a response to higher levels of H2O2 and subsequent POX mediated effects, were visualized structurally as a more elastic behaviour of irradiated tissues and rigidification of cell walls of treated roots, though puncture test did not reveal significant differences. Microscopy showed a continuum of thick cell walls in beet root tissue, which suffered slight modifications after irradiation, coherent with the bio-chemical results obtained. It can be concluded that irradiation doses of 1 or 2 kGy produced bio-chemical changes in cellular contents as well as in the cell wall constitutive networks which not necessarily could be sensed by consumers as it was objectively evaluated through a puncture test. At the same time, the mentioned changes involved an increase in the antioxidant capacity of red beet root tissue, showing that studied doses could be interesting to be used in the frame of a combined technique for red beet processing. © 2009 Elsevier Ltd. All rights reserved.

Registro:

Documento: Artículo
Título:Effects of gamma irradiation on bio-chemical and physico-chemical parameters of fresh-cut red beet (Beta vulgaris L. var. conditiva) root
Autor:Latorre, M.E.; Narvaiz, P.; Rojas, A.M.; Gerschenson, L.N.
Filiación:Departamento de Industrias, Facultad de Ciencias Exactas y Naturales (FCEN), Universidad de Buenos Aires (UBA) Ciudad Universitaria, Intendente Güiraldes 2620, 1428 Ciudad Autonoma de Buenos Aires, Argentina
Food Irradiation Section, Radiation Technology and Applications, Ezeiza Atomic Center, Argentina
Palabras clave:Bio-chemical parameters; Enzymes; Gamma irradiation; Mechanical parameters; Red beet; Bio-chemical parameters; Chemical parameters; Gamma irradiation; Mechanical parameters; Red beet; Adhesion; Amino acids; Calcium; Catalysts; Cell adhesion; Cells; Enzymes; Gamma rays; Industrial research; Irradiation; Plants (botany); Radiation; Stresses; Tissue; Value engineering; Chemical modification; Beta vulgaris; Beta vulgaris subsp. vulgaris
Año:2010
Volumen:98
Número:2
Página de inicio:178
Página de fin:191
DOI: http://dx.doi.org/10.1016/j.jfoodeng.2009.12.024
Título revista:Journal of Food Engineering
Título revista abreviado:J Food Eng
ISSN:02608774
CODEN:JFOED
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02608774_v98_n2_p178_Latorre

Referencias:

  • Ahn, H., Kim, J., Yook, H., Byun, M., Irradiation effects on free radical scavenging and antioxidative activity of phytic acid (2003) J. Food Sci., 68, pp. 2221-2224
  • AC, A.O., (2000) Official methods of analysis, (17th ed.). Method 994.12, , Association of Official Analytical Chemists, MD
  • Aquino-Bolaños, E.N., Mercado-Silva, E., Effects of polyphenol oxidase and peroxidase activity on browing of cut jicama (2004) Postharvest Biology and Technology, 33, pp. 275-283
  • Barreiro, J.A., Milano, M., Sandoval, A.J., Kinetics of colour change of double concentrated tomato paste during thermal treatment (1997) J. Food Eng., 33, pp. 359-371
  • Baydoun, E.A.H., Pavlencheva, N., Cumming, C.M., Waldron, K.W., Brett, C.T., Control of dehydrodiferulate cross-linking in pectins from sugar-beet tissues (2004) Phytochemistry, 65 (8), pp. 1107-1115
  • Bourne, M., (2002) Food texture and viscosity. Concept and Measurement. Second Edition, Chapter 4. Food Science and Technology, , International Series. Academic Press Publisher, New York pp. 113-126
  • Bradley, D.J., Kjellbom, P., Lamb, C.J., Elicitor- and wound-induced oxidative cross-linking of a proline-rich plant cell wall protein: A novel, rapid defense response (1992) Cell, 70, pp. 21-30
  • Brett, C., Waldron, K., (1996) Physiology and Biochemistry of Plant Cell Wall, , Chapman and Hall
  • Budini, R., Tonelli, D., Girotti, S., Analysis of total phenols using Prussian blue method (1980) J. Agric. Food Chem., 26 (6), pp. 1236-1238
  • Bunzel, M., Ralph, J., Marita, J., Stainhart, H., Identification of 4-O-5′coupled diferulic acid from insoluble cereal fiber (2000) J. Agric. Food Chem., 48, pp. 3166-3169
  • Cantos, E., Espin, J.C., Tomás-Barberán, F.A., Effect of wounding of phenolic enzymes in six minimally processed lettuce cultivars upon storage (2001) J. Agric. Food Chem., 49, pp. 322-330
  • Coimbra, M.A., Barros, A., Barros, M., Rutledge, D.N., Delgadillo, I., FTIR spectroscopy as a tool for the analysis of olive pulp cell-wall polysaccharide extracts (1999) Carbohydr. Res., 317, pp. 145-154
  • Coseteng, M.Y., Lee, C.Y., Changes in apple polyphenol concentrations in relation to degree of browning (1987) J. Food Sci., 52 (4), pp. 985-989
  • de Lara, R., Vazquez, M.I., Galan, P., Benavente, J., Modification of cellulose based membranes by gamma-radiation: effect of cellulose content (2006) J. Membr. Sci., 273 (1-2), pp. 25-30
  • Detopoulou, P., Panagiotakos, D.B., Antonopoulou, S., Pitsavos, C., Stefanadis, C., Dietary choline and betaine intakes in relation to concentrations of inflammatory markers in healthy adults: the ATTICA study (2008) Am. J. Clin. Nutr., 87 (2), pp. 424-430
  • Dizhbite, T., Telysheva, G., Jurkjane, V., Viesturs, U., Characterization of the radical scavenging activity of lignins-natural antioxidants (2004) Bioresour. Technol., 95 (3), pp. 309-317
  • Dongowski, G., Enzymatic degradation studies of pectin and cellulose from red beets (2001) Mol. Nutrit. Food Res, 45 (5), pp. 324-331
  • Duan, X., Su, X., You, Y., Qu, H., Li, Y., Jiang, Y., Effect of nitric oxide on pericarp browning of harvested longan fruit in relation to phenolic metabolism (2007) Food Chem., 104 (2), pp. 571-576
  • Dubois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.A., Smith, F., Colorimetric method for determination of sugars and related substances (1956) Anal. Chem., 28, pp. 350-356
  • Egley, E., Paul Jr., R.N., Vaughn, K.C., Duke, S.O., Role of peroxidase in the development of water-impermeable seed coats in Sida spinosa L. (1983) Plant, 157 (3), pp. 157-224
  • Escribano, J., Pedreño, M.A., García-Carmona, F., Muñoz, R., Characterization of the antiradical activity of betalains from Beta vulgaris L. (1998) Roots. Phytochem. Anal., 9 (3), pp. 124-127
  • Fan, X., Sokorai, K.J.B., Retention of quality and nutritional value of 13 fresh-cut vegetables treated with low-dose radiation (2008) J. Food Sci., 73 (7), pp. S367-S372
  • Farkas, J., Irradiation for better foods (2006) Trends Food Sci. Technol., 17, pp. 148-152
  • Faust, M., Chase, B.R., Massey Jr., L.M., The effect of ionizing radiation and diphenylamine treatment on glucose metabolism and membrane permeability of "Cortland' apples (1967) Proc. Am. Hort. Sci., 90, pp. 25-32
  • FDA, Irradiation in the production, processing and handling of food. Proposed rules (2007) Fed. Reg., 72 (64), pp. 16291-16306. , <http://www.cfsan.fda.gov/∼lrd/fr070404.html>
  • Filisetti-Cozzi, T.M.C.C., Carpita, N.C., Measurement of uronic acids without interference from neutral sugars (1991) Anal. Biochem., 197, pp. 157-162
  • Fissore, E.N., Ponce, N.M.A., Stortz, C.A., Rojas, A.M., Gerschenson, L.N., Characterization of fiber obtained from pumpkin (Cucumis moschata, Duch.) mesocarp through enzymatic treatment (2007) Food Sci. Technol. Int., 16 (1), pp. 1-7
  • Foley, D., Euper, M., Caporaso, F., Prakash, A., Irradiation and chlorination effectively reduces Escherichia coli O157:H7 inoculated on cilantro (Coriandrum sativum) without negatively affecting quality (2004) J. Food Prot., 67 (10), pp. 2092-2098
  • Fry, S.C., Cross-linking of matrix polymers in the growing cell walls of angiosperms (1986) Annu. Rev. Plant Physiol., 37, pp. 165-186
  • Fry, S.C., Oxidative scission of plant cell wall polysaccharides by ascorbate-induced hydroxyl radicals (1998) Biochem. J., 332, pp. 507-515
  • Gandia-Herrero, F., Escribano, J., Garcia-Carmona, F., Betaxanthins as substrates for tyrosinase. An approach to the role of tyrosinase in the biosynthetic pathway of betalains (2005) Plant Physiol., 138, pp. 421-432
  • Gliszczyńska-Świgło, A., Antioxidant activity of water soluble vitamins in the TEAC (trolox equivalent antioxidant capacity) and the FRAP (ferric reducing antioxidant power) assays (2006) Food Chem., 99 (1), pp. 131-136
  • Gomes, C., Moreira, R.G., Castell-Perez, M.E., Kim, J., Da Silva, P., Castillo, A., E-beam irradiation of bagged, ready-to-eat spinach leaves (Spinacea oleracea): an engineering approach (2008) J. Food Sci., 73 (2), pp. E95-E102
  • Grabber, J.H., How do lignin composition, structure, and cross-linking affect degradability? A review of cell wall model studies (2005) Crop. Sci., 45, pp. 820-831
  • Grassi, M., Lapasin, R., Pricl, S., A study of the rheological behaviour of scleroglucan weak gel systems (1996) Carbohydr. Polym., 29, pp. 169-181
  • Hallett, I.C., MacRae, E.A., Wegrzyn, T.F., Changes in kiwifruit cell wall ultrastructure and cell packing during postharvest ripening (1992) Int. J.Plant Sci., 153, pp. 49-60
  • Henderson, J., Ricker, R., Bidlingmeyer, B., Woodward, C., Rapid, Accurate, Sensitive, and Reproducible HPLC Analysis of Amino Acids (2000) Technical Report from Agilent, 8. , Technologies
  • Herbach, K., Stintzing, F., Carle, R., Impact of thermal treatment on colour and pigment pattern of red beet (Beta vulgaris L.) preparations (2004) J. Food Sci., 69 (6), pp. 491-498
  • Herbert, P., Barros, P., Ratola, N., Alves, A., HPLC Determination of amino acids in 4 musts and port wine using OPA/FMOC derivatives (2000) J. Food Sci., 65 (7), pp. 1130-1133
  • Combination processes for food irradiation (1998) Proceedings of the Final Research Co-ordination Meeting, Pretoria, South Africa, 27 February-3 March 1995 (Panel, , IAEA, International Atomic Energy Agency, Proceeding, Vienna
  • ICGFI, International Consultative Group on food, (1999) Facts about Food Irradiation, , IAEA, Vienna, Austria
  • Ilker, R., Szcsesniak, A.S., Structural and chemical bases for texture of plant foodstuffs (1990) J. Texture Stud., 21, pp. 1-36
  • Jackson, P., Paulo, S., Brownleader, M.D., Freire, P., Ricardo, C.P.P., An extensin peroxidase is associated with white-light inhibition of lupin (Lupinus albus) hypocotyl growth (1999) Aust. J. Plant Physiol., 26, pp. 313-326
  • Jarvis, M.C., Intercellular separation forces generated by intracellular pressure (1998) Plant Cell Environ., 21 (12), pp. 1307-1310
  • Jo, C., Son, J., Lee, H., Buym, M., Irradiation application for colour removal and purification of green tea leaves extract (2003) Radiat. Phys. Chem., 66, pp. 179-184
  • Kanner, J., Harel, S., Granit, R., Betalains. A new class of dietary cationized antioxidants (2001) J. Agric. Food Chem., 49, pp. 5178-5185
  • Knuthsen, P., Investigations on beetroot colours for the purpose of regulation (1981) Z. Lebensm. Unters. Forsch., 172, pp. 195-200
  • Kujala, T., Loponen, J., Klika, K., Pihlaja, K., Phenolics and betacyanins in red beetroot (Beta vulgaris) root: distribution and effect of cold storage on the content of total phenolics and three individual compounds (2000) J. Agric. Food Chem., 48, pp. 5338-5342
  • Kunstadt, P., Economic and technical considerations in food irradiation. Peter Chapter 16 (2001) Food Irradiation: Principles, Applications, pp. 415-442. , Molins R. (Ed), John Wiley and Sons Inc., NY, USA
  • Lacroix, M., Ouattara, B., Combined industrial processes with irradiation to assure innocuity and preservation of food products. A review (2000) Food Res. Int., 33, pp. 719-724
  • Langerak, D.I., Damen, G.A.A., Influence of irradiation on the keeping quality of pre-packed soup greens at 10 °C (1978) Food Preservation by Irradiation, 1. , International Atomic Energy Agency, Vienna, Austria pp. 275-282
  • Lee, J., Kim, J., Yook, H., Kang, K., Lee, W., Hwang, H., Buyn, M., Effects of gamma radiation on the allergenicity and antigenicity properties of milk proteins (2001) J. Food Prot., 64, pp. 272-276
  • Levigne, S., Ralet, M.C., Quéméner, B., Thibault, J.F., Isolation of diferulic bridges ester-linked to arabinan in sugar beet cell walls (2004) Carbohydr. Res., 339 (13), pp. 2315-2319
  • Lin, T.-T., Pitt, R.E., Rheology of apple and potato tissue as affected by cell turgor pressure (1986) J. Texture Stud., 17, pp. 291-313
  • Lowry, O.H., Rosebrough, N.J., Randall, R.J., Protein measurement with the Folin phenol reagent (1951) J. Biol. Chem., 193, pp. 265-275
  • Marangoni, A.G., Jackman, R.L., Stanley, D.W., Chilling associated softening of tomato fruit is related to increased pectinmethylesterase activity (1995) J. Food Sci., 60 (6), pp. 1277-1281
  • Marry, M., Roberts, K., Jopson, S.J., Huxham, I.M., Jarvis, M.C., Corsar, J., Robertson, E., McCann, M.C., Cell-cell adhesion in fresh sugar-beet root parenchyma requires both pectin esters and calcium cross-links (2006) Physiol. Plant., 126 (2), pp. 243-256
  • Martin-Cabrejas, M.A.M., Waldron, K.W., Selvendran, R.R., Cell wall changes in Spanish pear during ripening (1994) J. Plant Physiol., 144, pp. 541-548
  • Mc Evoy, H., Ross-Murphy, S.B., Clark, A.H., Large deformation and ultimate properties of biopolymer gels: 1. Single biopolymer component systems (1985) Polymer, 26, pp. 1483-1492
  • McCann, M.C., Hammouri, M., Wilson, R., Belton, P., Roberts, K., Fourier Transform Infrared Microspectroscopy is a new way to look at plant cell walls (1992) Plant Physiol., 100, pp. 1940-1947
  • Mobhammer, M.R., Maier, C., Stintzing, F.C., Carle, R., Impact of thermal treatment and storage on colour of yellow-orange cactus pear (Opuntia ficus-indica L. Mill. cv. 'Gialla') juices (2006) J. Food Sci., 71 (7), pp. 400-406
  • Mort, A.J., Moerschbacher, B.M., Pierce, M.L., Maness, N.O., Problems encountered during the extraction, purification, and chromatography of pectic fragments, and some solutions to them (1991) Carbohydr. Res., 215, pp. 219-227
  • Nagai, T., Ishizuka, S., Hara, H., Aoyama, Y., Dietary sugar beet fiber prevents the increase in aberrant crypt foci induced by gamma-irradiation in the colourectum of rats treated with an immunosuppressant (2000) J. Nutr., 130 (7), pp. 1682-1687
  • Narine, S.S., Marangoni, A.G., Fractal nature of fat crystal networks (1999) Phys. Rev. E, 59, pp. 1908-1920
  • Naumenko, I.V., Phillipov, M.P., Colourimetric method for determination of acetyl groups in pectic substances (1992) Akademi Nauk Republi Moldova Biologi i Khimie Nauki***, 1, pp. 56-59
  • Ng, A., Waldron, K.W., Effect of cooking and pre-cooking on cell wall chemistry in relation to firmness of carrot tissues (1997) J. Sci. Food Agric., 73, pp. 503-512
  • Ng, A., Parr, A.J., Ingham, M.L., Rigby, N.M., Waldron, K.W., Cell wall chemistry of carrots (Daucus carota L.; cv. Amstrong) during maturation and storage (1998) J. Agric. Food Chem., 46, pp. 2933-2939
  • Nguyen-the, C., Carlin, F., The microbiology of minimally processed fresh fruits and vegetables (1994) Crit. Rev. Food Sci. Nutr., 34, pp. 371-401
  • Niemira, B.A., Sommers, C.H., Fan, X., Suspending lettuce type influences recoverability and radiation sensitivity of Escherichia coli O157:H7 (2002) J. Food Prot., 65 (9), pp. 1388-1393
  • Niemira, B.A., Fan, X., Low dose irradiation of fresh and fresh-cut produce. safety, sensory, and shelf life. Chapter 10 (2006) Food Irradiation. Research and Technology, pp. 169-184. , Sommers C.A., and Fan X. (Eds), Blackwell Publishing and IFT Press, Oxford, UK
  • Niemira, B.A., Relative efficacy of sodium hypochlorite wash versus irradiation to inactivate Escherichia coli O157:H7 internalized in leaves of Romaine lettuce and baby spinach (2007) J. Food Prot., 71 (11), pp. 2526-2532
  • Noomhorm, A., Ilangantileke, S.G., Upadhyay, I.P., Karki, D.B., Apintanapong, M., Use of irradiation in combination with preservation techniques to extend the shelf life of tropical fruits and their products (1998) Combination processes for food irradiation, pp. 53-75. , International Atomic Energy Agency (Ed), International Atomic Energy Agency, Vienna, Austria
  • Nussinovitch, A., Peleg, M., Normand, M.D., A modified Maxwell and a non-exponential model for the characterization of the stress-relaxation of agar and alginate gels (1989) J. Food Sci., 54, pp. 1013-1016
  • Olthof, M.R., van Vliet, T., Boelsma, E., Verhoef, P., Low dose betaine supplementation leads to immediate and long term lowering of plasma homocysteine in healthy men and women (2003) J. Nutr., 133 (12), pp. 4135-4138
  • Oudgenoeg, G., Dirksen, E., Ingemann, S., Hilhorst, R., Gruppen, H., Boeriu, C.G., Piersma, S.R., Voragen, A.G.J., Horse radish peroxidase-catalyzed oligomerization of ferulic acid on a template of a tyrosine-containing tripeptide (2002) J. Biol. Chem., 277, pp. 21332-21334
  • Parr, A.J., Ng, A., Waldron, K.W., Ester-linked phenolic components of carrot cell walls (1997) J. Agric. Food Chem., 45 (7), pp. 2468-2471
  • Passardi, F., Longet, D., Penel, C., Dunand, C., The class III peroxidase multigenic family in rice and its evolution in land plants (2004) Phytochemistry, 65, pp. 1879-1893
  • Pedreño, M.A., Escribano, J., Correlation between antiradical activity and stability of betanine from Beta vulgaris L. roots under different pH, temperature and light conditions (2001) J. Sci. Food Agric., 81, pp. 627-631
  • Peleg, M., Calzada, J.F., Stress relaxation of deformed fruits and vegetables (1976) J. Food Sci., 41, pp. 1325-1329
  • Pitt, R.E., Chen, H.L., Time-dependent aspects of the strength and rheology of vegetative tissue (1983) Trans. ASAE, 26, pp. 1275-1280
  • Pouteau, C., Dole, P., Cathala, B., Averous, L., Boquillon, N., Antioxidant properties of lignin in polypropylene (2003) Polym. Degrad. Stab., 81, pp. 9-18
  • Prakash, A., Guner, A.R., Caporaso, F., Foley, D.M., Effects of low-dose gamma irradiation on the shelf life and quality characteristics of cut romaine lettuce packaged under modified atmosphere (2000) J. Food Sci., 65 (3), pp. 549-553
  • Prakash, A., Inthajak, P., Huibregtse, H., Caporaso, F., Foley, D.M., Effect of low dose gamma irradiation and conventional treatments on shelf life and quality characteristics of dicey celery (2000) J. Food Sci., 65, pp. 1070-1075
  • Price, N.J., Pinheiro, C., Soares, C.M., Ashford, D.A., Ricardo, C.P., Jackson, P.A., A Biochemical and Molecular Characterization of LEP1, an Extensin Peroxidase from Lupin (2003) J. Biol. Chem., 278 (42), pp. 41389-41399
  • Rastogi, N.K., Impact of gamma-irradiation on some mass transfer driven operations in food processing (2005) Radiat. Phys. Chem., 73, pp. 355-361
  • Regalado, C., Garcia-Almendarez, B.E., Duarte-Vanquez, M.A., Biotechnological applications of peroxidases (2004) Phytochem. Rev., 3 (1-2), pp. 243-256
  • Ribeiro, J.M., Silva Pereira, C., Soares, N.C., Vieira, A.M., Feijó, J.A., Jackson, P.A., The contribution of extensin network formation to rapid, hydrogen peroxide-mediated increases in grapevine callus wall resistance to fungal lytic enzymes (2006) J. Exp. Bot., 57 (9), pp. 2025-2035
  • Rojas, A.M., Gerschenson, L.N., Marangoni, A.G., Contributions of cellular components to the rheological behaviour of kiwifruit (2001) Food Res. Int., 34, pp. 189-195
  • Rojas, A.M., Delbon, M., Marangoni, A.G., Gerschenson, L.N., Contribution of cellular structure to the large and small deformation rheological behaviour of kiwifruit (2002) J. Food Sci., 67 (6), pp. 2143-2148
  • Roland, J.C., Cell wall differentiation and stages involved with intercellular gas space opening (1978) J. Cell Sci., 32 (1), pp. 325-336
  • Sánchez-Ferrer, A., Rodríguez-López, J.N., García-Cánovas, F., García-Carmona, F., Tyrosinase: a comprehensive review of its mechanism (1995) Biochim. Biophys. Acta, 1247, pp. 1-11
  • Schwartz, S.J., von Elbe, J.H., Jackman, R.L., Smith, J.L., Quantitative determination of individual betacyanin pigments by high performance liquid chromatography (1980) J. Agric. Food Chem., 28, pp. 540-543
  • Sepúlveda-Jiménez, G., Rueda-Benítez, P., Porta, H., Rocha-Sosa, M., Betacyanin synthesis in red beet (Beta vulgaris) leaves induced by wounding and bacterial infiltration is preceded by an oxidative burst (2004) Phys. Mol. Plant Pathol., 64, pp. 125-133
  • Shui, G., Leong, L.P., Residue from star fruit as valuable source for functional food ingredients and antioxidant nutraceuticals (2006) Food Chem., 97, pp. 277-284
  • Stadelmann, E., Evaluation of turgidity, plasmolysis and deplasmolysis of plant cells (1966) Methods in Cell Physiology, II, pp. 143-216. , Prescott D.M. (Ed), Academic Press, New York
  • Strack, D., Vogt, T., Schliemann, W., Recent advances in betalain research (2003) Phytochemistry, 62 (3), pp. 247-269
  • Strasser, G.R., Amado, R., Pectic substances from red beet (Beta vulgaris conditiva). Part I. Structural analysis of rhamnogalacturonan I using enzymic degradation and methylation analysis (2001) Carbohydr. Polym., 44, pp. 63-70
  • Synytsya, A., Copíková, J., Matějka, P., Machovič, V., Fourier transform Raman and infrared spectroscopy of pectins (2003) Carbohydr. Polym., 54, pp. 97-106
  • Thakur, B., Singh, R., Combination processes in food irradiation (1995) Trends Food Sci.Techn., 6, pp. 7-11
  • Thayer, D.W., Food Irradiation: Benefits and concerns (1990) J. Food Qual., 13, pp. 147-169
  • Toivonen, P.M.A., Brummell, D.A., Biochemical bases of appearance and texture changes in fresh-cut fruit and vegetables (2008) Postharvest. Biol. Technol., 48 (1), pp. 1-14
  • Vincken, J.P., Schols, H.A., Oomen, R.J.F.J., Beldman, G., Visser, R.G.F., Voragen, A.G.J., (2003) Pectin: the hairy thing. In: Advances in pectin and pectinase research, , Kluwer Academic Publishers, Voragen A. G. J., Schols H. A., Visser R. eds., Dordrecht pp. 47-59
  • Waje, C.K., Jun, S.Y., Lee, Y.K., Kim, B.N., Han, D.H., Jo, C., Kwon, J.H., Microbial quality assessment and pathogen inactivation by electron beam and gamma irradiation of commercial seed sprouts (2009) Food Control, 20, pp. 200-204
  • Waldron, K.W., Smith, K.C., Parr, A.J., Ng, A., Parker, M.L., New approaches to understanding and controlling cell separation in relation to fruit and vegetable texture (1997) Trends Food Sci Technol., 8, pp. 213-221
  • Waldron, K.W., Ng, A., Parker, M.L., Parr, A.J., Ferulic acid dehydrodimers in the cell walls of Beta vulgaris and their possible role in texture (1997) J. Sci. Food Agric., 74, pp. 221-228
  • Walton, D.C., Sondheimer, E., Effects of Abscisin II on Phenylalanine Ammonia-Lyase Activity in Excised Bean Axes (1968) Plant Physiol., 43 (3), pp. 467-469
  • Wang, Z., Ma, Y., Zhao, G., Liao, X., Chen, F., Wu, J., Chen, J., Hu, X., Influence of gamma irradiation on enzyme, microorganism and flavor of cantaloupe (Cucumis melo L.) juice (2006) J. Food Sci., 71 (6), pp. M215-M220
  • Wilson, R.H., Smith, A.C., Kacurakova, M., Saunders, P.K., Wellner, N., Waldron, K.W., The mechanical properties and molecular dynamics of plant cell wall polysaccharides studied by Fourier-transform infrared spectroscopy (2000) Plant Physiol., 124, pp. 397-405
  • Wood, P.J., Siddiqui, I.R., Determination of methanol and its application for measurement of pectin ester content and pectin methyl esterase activity (1971) Anal. Biochem., 39, pp. 418-428
  • Xuan, L., Yanxiang, G., Xiaoting, P., Bin, Y., Honggao, X., Jian, Z., Inactivation of peroxidase and polyphenol osidase in red beet (Beta vulgaris L.) extract with high pressure carbon dioxide (2008) Innovative Food Sci. Emerg. Technol., 9, pp. 24-31
  • Yoshikazu, T., Nobuhiro, S., Akemi, O., Biosynthesis of plant pigments: anthocyanins, betalains and carotenoids (2008) The Plant J., 54 (4), pp. 733-749

Citas:

---------- APA ----------
Latorre, M.E., Narvaiz, P., Rojas, A.M. & Gerschenson, L.N. (2010) . Effects of gamma irradiation on bio-chemical and physico-chemical parameters of fresh-cut red beet (Beta vulgaris L. var. conditiva) root. Journal of Food Engineering, 98(2), 178-191.
http://dx.doi.org/10.1016/j.jfoodeng.2009.12.024
---------- CHICAGO ----------
Latorre, M.E., Narvaiz, P., Rojas, A.M., Gerschenson, L.N. "Effects of gamma irradiation on bio-chemical and physico-chemical parameters of fresh-cut red beet (Beta vulgaris L. var. conditiva) root" . Journal of Food Engineering 98, no. 2 (2010) : 178-191.
http://dx.doi.org/10.1016/j.jfoodeng.2009.12.024
---------- MLA ----------
Latorre, M.E., Narvaiz, P., Rojas, A.M., Gerschenson, L.N. "Effects of gamma irradiation on bio-chemical and physico-chemical parameters of fresh-cut red beet (Beta vulgaris L. var. conditiva) root" . Journal of Food Engineering, vol. 98, no. 2, 2010, pp. 178-191.
http://dx.doi.org/10.1016/j.jfoodeng.2009.12.024
---------- VANCOUVER ----------
Latorre, M.E., Narvaiz, P., Rojas, A.M., Gerschenson, L.N. Effects of gamma irradiation on bio-chemical and physico-chemical parameters of fresh-cut red beet (Beta vulgaris L. var. conditiva) root. J Food Eng. 2010;98(2):178-191.
http://dx.doi.org/10.1016/j.jfoodeng.2009.12.024