Artículo
Abstract:
Considering both the wide spread utilization of porphyrins as photodiagnostic and phototherapeutic agents for tumours, and the limited information about the heme biosynthetic pathway in neoplastic tissues, a study of porphyrin biosynthesis in human breast adenocarcinoma, determining first the optimal working conditions, was initiated. The effect of temperature, maintenance time and the amount of precursor (δ-aminolevulinic acid, ALA) added and uptaken by normal and neoplastic tissue on the formation of porphyrins was determined. The optimal initial ALA concentration was approximately 34 μg/ml, while optimal temperature and maintenance time were 37°C and 24 h respectively. Under these conditions, porphyrin biosynthesis in tumoral tissue was about five times higher than normal. The functionality of the heme enzymes was indirectly measured by means of porphyrin chromatographic separation. Starting with ALA as substrate it was shown that the heme biosynthetic pathway was functional in the neoplastic tissue, up to the formation of protoporphyrinogen.
Registro:
| Documento: | Artículo |
| Título: | Porphyrin bioasynthesis in human breast cancer. Preliminary mimetic in vitro studies |
| Autor: | Navone, N.M.; Frisardi, A.L.; Resnik, E.R.; Del Batlle, C.A.M.; Polo, C.F. |
| Filiación: | Centro de Investigaciones Porfirinas y Porfirias (CIPYP), Ciudad Universitaria, 1428 Buenos Aires, Argentina |
| Palabras clave: | aminolevulinic acid; porphyrin; breast cancer; controlled study; drug therapy; human; human cell; in vitro study; organ culture; priority journal |
| Año: | 1988 |
| Volumen: | 16 |
| Número: | 2 |
| Página de inicio: | 61 |
| Página de fin: | 62 |
| Título revista: | Medical Science Research |
| Título revista abreviado: | MED. SCI. RES. |
| ISSN: | 02698951 |
| CODEN: | MSCRE |
| CAS: | aminolevulinic acid, 106-60-5; porphyrin, 24869-67-8 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02698951_v16_n2_p61_Navone |
Citas:
---------- APA ----------
Navone, N.M., Frisardi, A.L., Resnik, E.R., Del Batlle, C.A.M. & Polo, C.F. (1988) . Porphyrin bioasynthesis in human breast cancer. Preliminary mimetic in vitro studies. Medical Science Research, 16(2), 61-62.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02698951_v16_n2_p61_Navone [ ]
---------- CHICAGO ----------
Navone, N.M., Frisardi, A.L., Resnik, E.R., Del Batlle, C.A.M., Polo, C.F. "Porphyrin bioasynthesis in human breast cancer. Preliminary mimetic in vitro studies" . Medical Science Research 16, no. 2 (1988) : 61-62.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02698951_v16_n2_p61_Navone [ ]
---------- MLA ----------
Navone, N.M., Frisardi, A.L., Resnik, E.R., Del Batlle, C.A.M., Polo, C.F. "Porphyrin bioasynthesis in human breast cancer. Preliminary mimetic in vitro studies" . Medical Science Research, vol. 16, no. 2, 1988, pp. 61-62.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02698951_v16_n2_p61_Navone [ ]
---------- VANCOUVER ----------
Navone, N.M., Frisardi, A.L., Resnik, E.R., Del Batlle, C.A.M., Polo, C.F. Porphyrin bioasynthesis in human breast cancer. Preliminary mimetic in vitro studies. MED. SCI. RES. 1988;16(2):61-62.Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02698951_v16_n2_p61_Navone [ ]
