Artículo

Espelt, M.V.; Croci, D.O.; Bacigalupo, M.L.; Carabias, P.; Manzi, M.; Elola, M.T.; Muñoz, M.C.; Dominici, F.P.; Wolfenstein-Todel, C.; Rabinovich, G.A.; Troncoso, M.F. "Novel roles of galectin-1 in hepatocellular carcinoma cell adhesion, polarization, and in vivo tumor growth" (2011) Hepatology. 53(6):2097-2106
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Abstract:

Galectin-1 (Gal-1), a widely expressed β-galactoside-binding protein, exerts pleiotropic biological functions. Gal-1 is up-regulated in hepatocarcinoma cells, although its role in liver pathophysiology remains uncertain. We investigated the effects of Gal-1 on HepG2 hepatocellular carcinoma (HCC) cell adhesion and polarization. Soluble and immobilized recombinant Gal-1 (rGal-1) promoted HepG2 cell adhesion to uncoated plates and also increased adhesion to laminin. Antibody-mediated blockade experiments revealed the involvement of different integrins as critical mediators of these biological effects. In addition, exposure to rGal-1 markedly accelerated the development of apical bile canaliculi as shown by TRITC-phalloidin labeling and immunostaining for multidrug resistance associated-protein 2 (MRP2). Notably, rGal-1 did not interfere with multidrug resistance protein 1/P-glycoprotein or MRP2 apical localization, neither with transfer nor secretion of 5-chloromethylfluorescein diacetate through MRP2. Stimulation of cell adhesion and polarization by rGal-1 was abrogated in the presence of thiodigalactoside, a galectin-specific sugar, suggesting the involvement of protein-carbohydrate interactions in these effects. Additionally, Gal-1 effects were abrogated in the presence of wortmmanin, PD98059 or H89, suggesting involvement of phosphoinositide 3-kinase (PI3K), mitogen-activated protein kinase and cyclic adenosine monophosphate-dependent protein kinase signaling pathways in these functions. Finally, expression levels of this endogenous lectin correlated with HCC cell adhesion and polarization and up-regulation of Gal-1-favored growth of hepatocarcinoma in vivo. Conclusion: Our results provide the first evidence of a role of Gal-1 in modulating HCC cell adhesion, polarization, and in vivo tumor growth, with critical implications in liver pathophysiology. Copyright © 2011 American Association for the Study of Liver Diseases.

Registro:

Documento: Artículo
Título:Novel roles of galectin-1 in hepatocellular carcinoma cell adhesion, polarization, and in vivo tumor growth
Autor:Espelt, M.V.; Croci, D.O.; Bacigalupo, M.L.; Carabias, P.; Manzi, M.; Elola, M.T.; Muñoz, M.C.; Dominici, F.P.; Wolfenstein-Todel, C.; Rabinovich, G.A.; Troncoso, M.F.
Filiación:Instituto de Química y Fisicoquímica Biológicas, Consejo Nacional de Investigaciones Científicas y Técnicas, Depto. de Quimica Biológica, Facultad de Farmacia y Bioquímica, Buenos Aires, Argentina
Instituto de Biología y Medicina Experimental, Consejo Nacional de Investigaciones Cientificas y Tecnicas, Depto. de Quimica Biologica, Fac. de Ciencias Exactas y Naturales, Univ. de Buenos Aires, Buenos Aires, Argentina
Palabras clave:beta galactoside; galectin 1; integrin; laminin; lectin; multidrug resistance protein 2; phalloidin; sugar; thioglycoside; article; cell adhesion; controlled study; human; human cell; immunohistochemistry; intrahepatic bile duct; liver cell carcinoma; pathophysiology; pleiotropy; priority journal; protein localization; tumor growth; Carcinoma, Hepatocellular; Cell Adhesion; Cell Line, Tumor; Cell Polarity; Cell Proliferation; Cyclic AMP-Dependent Protein Kinases; Galectin 1; Humans; Liver Neoplasms; Mitogen-Activated Protein Kinases; Multidrug Resistance-Associated Proteins; Phosphatidylinositol 3-Kinases; Signal Transduction
Año:2011
Volumen:53
Número:6
Página de inicio:2097
Página de fin:2106
DOI: http://dx.doi.org/10.1002/hep.24294
Título revista:Hepatology
Título revista abreviado:Hepatology
ISSN:02709139
CODEN:HPTLD
CAS:galectin 1, 258495-34-0; laminin, 2408-79-9; multidrug resistance protein 2, 256503-65-8; phalloidin, 17466-45-4; Cyclic AMP-Dependent Protein Kinases, 2.7.11.11; Galectin 1; Mitogen-Activated Protein Kinases, 2.7.11.24; Multidrug Resistance-Associated Proteins; Phosphatidylinositol 3-Kinases, 2.7.1.-; multidrug resistance-associated protein 2
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02709139_v53_n6_p2097_Espelt

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Citas:

---------- APA ----------
Espelt, M.V., Croci, D.O., Bacigalupo, M.L., Carabias, P., Manzi, M., Elola, M.T., Muñoz, M.C.,..., Troncoso, M.F. (2011) . Novel roles of galectin-1 in hepatocellular carcinoma cell adhesion, polarization, and in vivo tumor growth. Hepatology, 53(6), 2097-2106.
http://dx.doi.org/10.1002/hep.24294
---------- CHICAGO ----------
Espelt, M.V., Croci, D.O., Bacigalupo, M.L., Carabias, P., Manzi, M., Elola, M.T., et al. "Novel roles of galectin-1 in hepatocellular carcinoma cell adhesion, polarization, and in vivo tumor growth" . Hepatology 53, no. 6 (2011) : 2097-2106.
http://dx.doi.org/10.1002/hep.24294
---------- MLA ----------
Espelt, M.V., Croci, D.O., Bacigalupo, M.L., Carabias, P., Manzi, M., Elola, M.T., et al. "Novel roles of galectin-1 in hepatocellular carcinoma cell adhesion, polarization, and in vivo tumor growth" . Hepatology, vol. 53, no. 6, 2011, pp. 2097-2106.
http://dx.doi.org/10.1002/hep.24294
---------- VANCOUVER ----------
Espelt, M.V., Croci, D.O., Bacigalupo, M.L., Carabias, P., Manzi, M., Elola, M.T., et al. Novel roles of galectin-1 in hepatocellular carcinoma cell adhesion, polarization, and in vivo tumor growth. Hepatology. 2011;53(6):2097-2106.
http://dx.doi.org/10.1002/hep.24294