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Abstract:

The aim of the present work was to analyze the direct effect of VEGF in follicular cell proliferation, apoptosis and activation of the PI3K/AKT and ERK/MEK signaling pathways in early antral follicles or granulosa cells. Antral follicles or granulosa cells were isolated from prepubertal female Sprague Dawley rats treated with DES.VEGF directly stimulates follicular cell proliferation and it also decreases apoptosis by inhibiting caspase 3 activation. In addition, VEGF increases the proliferation and inhibits the apoptosis of isolated granulosa cells in culture. VEGF activates the PI3K/AKT pathway evidenced by an increase in AKT phosphorylation levels and induces the phosphorylation of ERK1/2 in cultured antral follicles. These results demonstrate for the first time that VEGF has a proliferative and cytoprotective role in early antral follicles and in granulosa cells isolated from DES treated prepubertal rats and suggest that PI3K/AKT and ERK/MEK signaling pathways are involved in these processes. © 2010 Elsevier Ireland Ltd.

Registro:

Documento: Artículo
Título:Direct survival role of vascular endothelial growth factor (VEGF) on rat ovarian follicular cells
Autor:Irusta, G.; Abramovich, D.; Parborell, F.; Tesone, M.
Filiación:Instituto de Biología y Medicina Experimental (IByME), CONICET, Buenos Aires, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Apoptosis; Ovary; Proliferation; Signaling pathways; VEGF; diethylstilbestrol; DNA; mitogen activated protein kinase; mitogen activated protein kinase 1; mitogen activated protein kinase 3; phosphatidylinositol 3 kinase; protein kinase B; vasculotropin; animal cell; animal cell culture; apoptosis; article; cell isolation; cell proliferation; cell survival; controlled study; DNA fragmentation; DNA isolation; enzyme activation; enzyme phosphorylation; female; granulosa cell; nick end labeling; nonhuman; ovary follicle cell; priority journal; rat; Western blotting; Animals; Apoptosis; Cell Proliferation; Cell Survival; Cells, Cultured; Enzyme Activation; Extracellular Signal-Regulated MAP Kinases; Female; Granulosa Cells; Oncogene Protein v-akt; Ovarian Follicle; Rats; Rats, Sprague-Dawley; Sexual Maturation; Signal Transduction; Vascular Endothelial Growth Factor A
Año:2010
Volumen:325
Número:1-2
Página de inicio:93
Página de fin:100
DOI: http://dx.doi.org/10.1016/j.mce.2010.04.018
Título revista:Molecular and Cellular Endocrinology
Título revista abreviado:Mol. Cell. Endocrinol.
ISSN:03037207
CODEN:MCEND
CAS:DNA, 9007-49-2; diethylstilbestrol, 30498-85-2, 56-53-1; mitogen activated protein kinase, 142243-02-5; mitogen activated protein kinase 1, 137632-08-7; mitogen activated protein kinase 3, 137632-07-6; phosphatidylinositol 3 kinase, 115926-52-8; protein kinase B, 148640-14-6; vasculotropin, 127464-60-2; Extracellular Signal-Regulated MAP Kinases, 2.7.11.24; Oncogene Protein v-akt, 2.7.11.1; Vascular Endothelial Growth Factor A; vascular endothelial growth factor A, rat
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v325_n1-2_p93_Irusta

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Citas:

---------- APA ----------
Irusta, G., Abramovich, D., Parborell, F. & Tesone, M. (2010) . Direct survival role of vascular endothelial growth factor (VEGF) on rat ovarian follicular cells. Molecular and Cellular Endocrinology, 325(1-2), 93-100.
http://dx.doi.org/10.1016/j.mce.2010.04.018
---------- CHICAGO ----------
Irusta, G., Abramovich, D., Parborell, F., Tesone, M. "Direct survival role of vascular endothelial growth factor (VEGF) on rat ovarian follicular cells" . Molecular and Cellular Endocrinology 325, no. 1-2 (2010) : 93-100.
http://dx.doi.org/10.1016/j.mce.2010.04.018
---------- MLA ----------
Irusta, G., Abramovich, D., Parborell, F., Tesone, M. "Direct survival role of vascular endothelial growth factor (VEGF) on rat ovarian follicular cells" . Molecular and Cellular Endocrinology, vol. 325, no. 1-2, 2010, pp. 93-100.
http://dx.doi.org/10.1016/j.mce.2010.04.018
---------- VANCOUVER ----------
Irusta, G., Abramovich, D., Parborell, F., Tesone, M. Direct survival role of vascular endothelial growth factor (VEGF) on rat ovarian follicular cells. Mol. Cell. Endocrinol. 2010;325(1-2):93-100.
http://dx.doi.org/10.1016/j.mce.2010.04.018