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Abstract:

1. 1. A non-sedimentable fraction of potato tuber has been found to catalyze [14C] glucose transfer from [14C] glucose 1-phosphate to an endogenous proteic acceptor in the absence of added primer. This transfer is activated by Mn2+. 2. 2. The labeled glucosylated product formed is trichloroacetic acid insoluble and sensitive to proteolytic and amylolytic digestions. It appears to be a glucoprotein with glucosyl chains bound to the peptide portion of the molecule through an unknown linkage. 3. 3. The carbohydrate portion of the glucoprotein can be released by prolonged incubations with the enzymatic preparation, and becomes in turn, trichloroacetic acid soluble and alcohol precipitable. 4. 4. Both products, the glucoprotein as well as the α-1,4-glucan that seems to arise from the enzymatic cleavage of the former, can be used as primers by the transglucosylating system with ADP[14C] glucose, UDP[14C] glucose or GDP[14C] glucose as glucosyl donors. The results presented in this paper are the first demonstration of soluble glucosyl transferases with the same glucose donor specificity to that of the particulate starch synthetase. 5. 5. This report presents further evidence in favor of the assumption of a glucoproteic intermediate in α-1,4-glucan synthesis initiation. © 1975.

Registro:

Documento: Artículo
Título:Biosynthesis of starch. Formation of a glucoproteic acceptor by a potato non-sedimentable preparation
Autor:Tandecarz, J.; Lavintman, N.; Cardini, C.E.
Filiación:Instituto de Investigaciones Bioquímicas Fundacíon Campomar, Facultad de Ciencias Exactas y Naturales, Obligado 2490 Buenos Aires (28), Argentina
Palabras clave:adenosine diphosphate; glucose 1 phosphate; glucosyltransferase; glycoprotein; guanosine diphosphate; starch; uridine diphosphate; glucose c 14; in vitro study; plant; theoretical study; Adenosine Diphosphate Glucose; Glucosephosphates; Glucosyltransferases; Guanosine Diphosphate Sugars; Kinetics; Manganese; Plant Proteins; Plants; Starch; Uridine Diphosphate Glucose
Año:1975
Volumen:399
Número:2
Página de inicio:345
Página de fin:355
DOI: http://dx.doi.org/10.1016/0304-4165(75)90263-9
Título revista:BBA - General Subjects
Título revista abreviado:Biochim. Biophys. Acta Gen. Subj.
ISSN:03044165
CODEN:BBGSB
CAS:adenosine diphosphate, 20398-34-9, 58-64-0; glucose 1 phosphate, 59-56-3; glucosyltransferase, 9031-48-5; guanosine diphosphate, 146-91-8; starch, 9005-25-8, 9005-84-9; uridine diphosphate, 58-98-0; Adenosine Diphosphate Glucose, 2140-58-1; Glucosephosphates; Glucosyltransferases, EC 2.4.1.-; Guanosine Diphosphate Sugars; Manganese, 7439-96-5; Plant Proteins; Starch, 9005-25-8; Uridine Diphosphate Glucose, 133-89-1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03044165_v399_n2_p345_Tandecarz

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Citas:

---------- APA ----------
Tandecarz, J., Lavintman, N. & Cardini, C.E. (1975) . Biosynthesis of starch. Formation of a glucoproteic acceptor by a potato non-sedimentable preparation. BBA - General Subjects, 399(2), 345-355.
http://dx.doi.org/10.1016/0304-4165(75)90263-9
---------- CHICAGO ----------
Tandecarz, J., Lavintman, N., Cardini, C.E. "Biosynthesis of starch. Formation of a glucoproteic acceptor by a potato non-sedimentable preparation" . BBA - General Subjects 399, no. 2 (1975) : 345-355.
http://dx.doi.org/10.1016/0304-4165(75)90263-9
---------- MLA ----------
Tandecarz, J., Lavintman, N., Cardini, C.E. "Biosynthesis of starch. Formation of a glucoproteic acceptor by a potato non-sedimentable preparation" . BBA - General Subjects, vol. 399, no. 2, 1975, pp. 345-355.
http://dx.doi.org/10.1016/0304-4165(75)90263-9
---------- VANCOUVER ----------
Tandecarz, J., Lavintman, N., Cardini, C.E. Biosynthesis of starch. Formation of a glucoproteic acceptor by a potato non-sedimentable preparation. Biochim. Biophys. Acta Gen. Subj. 1975;399(2):345-355.
http://dx.doi.org/10.1016/0304-4165(75)90263-9