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Meinardi, E.; Azcurra, J.M.; Florin-Christensen, M.; Florin-Christensen, J. "Coelenterolysin: a hemolytic polypeptide associated with the coelenteric fluid of sea anemones" (1994) Comparative Biochemistry and Physiology -- Part B: Biochemistry and. 109(1):153-161
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Abstract:

The gastrovascular fluids of the sea anemone Phymactis clematis display strong hemolytic activity, which has basic pH optimum, is thermolabile and is sensitive to proteases. The hemolytic agent from the gastrovascular fluid was partially purified by ammonium sulfate precipitation, chromatography on Dowex-50W cation exchanger and gel filtration on Sephadex G-50. A single peak elutes from the latter with an estimated mol. wt of 18,000. This elution pattern is unaffected if the chromatography is carried out in the presence of 5 M urea. These results indicate that the hemolytic activity is due to a single peptide or a group of peptides of similar size, which we here designate as "coelenterolysin". Coelenterolysin is also present in sea anemone tissue homogenates, is different from the nematocyst toxin and is not associated with phospholipase activities. It is inhibited by sphingomyelin. This is the first report of hemolytic polypeptides associated with the coelenteric fluid of sea anemones. Coelenterolysin may have a role in extracellular digestion, defense against predators and invasion of the coelenteron by foreign organisms. © 1994.

Registro:

Documento: Artículo
Título:Coelenterolysin: a hemolytic polypeptide associated with the coelenteric fluid of sea anemones
Autor:Meinardi, E.; Azcurra, J.M.; Florin-Christensen, M.; Florin-Christensen, J.
Filiación:Cátedra de Biología Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, 1428 Buenos Aires, Argentina
Instituto de Neurociencia (INEUCI), Casilla de Correo 137, 1663 San Miguel, Argentina
Palabras clave:Coelenterolysin; Gastrovascular fluid; Phymactis clematis; coelenterolysin; sea anemone toxin; unclassified drug; article; hemolysis; nonhuman; priority journal; sea anemone; Actiniaria; Anemone; Clematis; Phymactis clematis
Año:1994
Volumen:109
Número:1
Página de inicio:153
Página de fin:161
DOI: http://dx.doi.org/10.1016/0305-0491(94)90152-X
Título revista:Comparative Biochemistry and Physiology -- Part B: Biochemistry and
ISSN:03050491
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03050491_v109_n1_p153_Meinardi

Referencias:

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Citas:

---------- APA ----------
Meinardi, E., Azcurra, J.M., Florin-Christensen, M. & Florin-Christensen, J. (1994) . Coelenterolysin: a hemolytic polypeptide associated with the coelenteric fluid of sea anemones. Comparative Biochemistry and Physiology -- Part B: Biochemistry and, 109(1), 153-161.
http://dx.doi.org/10.1016/0305-0491(94)90152-X
---------- CHICAGO ----------
Meinardi, E., Azcurra, J.M., Florin-Christensen, M., Florin-Christensen, J. "Coelenterolysin: a hemolytic polypeptide associated with the coelenteric fluid of sea anemones" . Comparative Biochemistry and Physiology -- Part B: Biochemistry and 109, no. 1 (1994) : 153-161.
http://dx.doi.org/10.1016/0305-0491(94)90152-X
---------- MLA ----------
Meinardi, E., Azcurra, J.M., Florin-Christensen, M., Florin-Christensen, J. "Coelenterolysin: a hemolytic polypeptide associated with the coelenteric fluid of sea anemones" . Comparative Biochemistry and Physiology -- Part B: Biochemistry and, vol. 109, no. 1, 1994, pp. 153-161.
http://dx.doi.org/10.1016/0305-0491(94)90152-X
---------- VANCOUVER ----------
Meinardi, E., Azcurra, J.M., Florin-Christensen, M., Florin-Christensen, J. Coelenterolysin: a hemolytic polypeptide associated with the coelenteric fluid of sea anemones. 1994;109(1):153-161.
http://dx.doi.org/10.1016/0305-0491(94)90152-X