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Abstract:

Ca v2.1 channels (P/Q-type) play a prominent role in controlling neurotransmitter release. Transgenic mice in which the α1A pore-forming subunit of Ca v2.1 channels is ablated (KO) provide a powerful tool to study Ca v2.1 function in synaptic transmission in vivo. Whole-cell patch clamp was used to measure inhibitory glycinergic postsynaptic currents (IPSCs) from the lateral superior olive (LSO). Comparing wild-type (WT) and KO mice, we investigated the relevance of P/Q-type calcium channels at a glycinergic synapse mediated by multiple types of Ca 2+ channels, in opposition to synapses where only this type of Ca 2+ channels are in charge of transmitter release. We found that in KO mice, N-type and L-type Ca 2+ channels control synaptic transmission, resulting in a functional but reduced glycinergic transmitter release. Pair pulse facilitation of synaptic currents is retained in KO mice, even when synaptic transmission is driven by either N or L-type calcium channels alone, in contrast with lack of this phenomenon in other synapses which are exclusively mediated by P/Q-type channels. Thus, pointing a difference between P/Q- and N-type channels present in single or multiple types of calcium channels driven synapses. Significant alterations in short-term synaptic plasticity were observed. KO mice exhibited a stronger short term depression (STD) of IPSCs during repetitive stimulation at high frequency and recovered with a larger time constant compared to WT mice. Finally, transmitter release at the LSO synapse from KO mice was strongly modulated by presynaptic GTP-binding protein-coupled receptor γ-aminobutyric acid type B (GABA B). © 2011.

Registro:

Documento: Artículo
Título:P/Q-type calcium channel ablation in a mice glycinergic synapse mediated by multiple types of Ca 2+ channels alters transmitter release and short term plasticity
Autor:Giugovaz-Tropper, B.; González-Inchauspe, C.; Di Guilmi, M.N.; Urbano, F.J.; Forsythe, I.D.; Uchitel, O.D.
Filiación:Instituto de Fisiología, Biología molecular y Neurociencias, Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Universidad de Buenos Aires, Departamento de Fisiologia, Biologia Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria, 1428, Buenos Aires, Argentina
Neurotoxicity at the Synaptic Interface, MRC Toxicology Unit, University of Leicester, LE1 9HN, United Kingdom
Palabras clave:CaV2.1 KO mice; Inhibitory glycinergic postsynaptic currents; P/Q-type calcium channels; Pair pulse facilitation; Short term synaptic plasticity; 4 aminobutyric acid B receptor; baclofen; calcium channel L type; calcium channel N type; calcium channel P type; calcium channel Q type; glycine receptor; guanine nucleotide binding protein; animal cell; article; brain function; controlled study; electroencephalogram; facilitation; nerve cell plasticity; nerve stimulation; neurotransmitter release; nonhuman; priority journal; protein expression; protein function; short term depression; synapse; synaptic transmission; Animals; Brain Stem; Calcium Channels; Calcium Channels, P-Type; Calcium Channels, Q-Type; Excitatory Postsynaptic Potentials; Glycine; Inhibitory Postsynaptic Potentials; Mice; Mice, Knockout; Mice, Transgenic; Neuronal Plasticity; Neurons; Neurotransmitter Agents; Organ Culture Techniques; Patch-Clamp Techniques; Synapses; Synaptic Transmission
Año:2011
Volumen:192
Página de inicio:219
Página de fin:230
DOI: http://dx.doi.org/10.1016/j.neuroscience.2011.06.021
Título revista:Neuroscience
Título revista abreviado:Neuroscience
ISSN:03064522
CODEN:NRSCD
CAS:baclofen, 1134-47-0; Calcium Channels; Calcium Channels, P-Type; Calcium Channels, Q-Type; Glycine, 56-40-6; Neurotransmitter Agents
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03064522_v192_n_p219_GiugovazTropper

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Citas:

---------- APA ----------
Giugovaz-Tropper, B., González-Inchauspe, C., Di Guilmi, M.N., Urbano, F.J., Forsythe, I.D. & Uchitel, O.D. (2011) . P/Q-type calcium channel ablation in a mice glycinergic synapse mediated by multiple types of Ca 2+ channels alters transmitter release and short term plasticity. Neuroscience, 192, 219-230.
http://dx.doi.org/10.1016/j.neuroscience.2011.06.021
---------- CHICAGO ----------
Giugovaz-Tropper, B., González-Inchauspe, C., Di Guilmi, M.N., Urbano, F.J., Forsythe, I.D., Uchitel, O.D. "P/Q-type calcium channel ablation in a mice glycinergic synapse mediated by multiple types of Ca 2+ channels alters transmitter release and short term plasticity" . Neuroscience 192 (2011) : 219-230.
http://dx.doi.org/10.1016/j.neuroscience.2011.06.021
---------- MLA ----------
Giugovaz-Tropper, B., González-Inchauspe, C., Di Guilmi, M.N., Urbano, F.J., Forsythe, I.D., Uchitel, O.D. "P/Q-type calcium channel ablation in a mice glycinergic synapse mediated by multiple types of Ca 2+ channels alters transmitter release and short term plasticity" . Neuroscience, vol. 192, 2011, pp. 219-230.
http://dx.doi.org/10.1016/j.neuroscience.2011.06.021
---------- VANCOUVER ----------
Giugovaz-Tropper, B., González-Inchauspe, C., Di Guilmi, M.N., Urbano, F.J., Forsythe, I.D., Uchitel, O.D. P/Q-type calcium channel ablation in a mice glycinergic synapse mediated by multiple types of Ca 2+ channels alters transmitter release and short term plasticity. Neuroscience. 2011;192:219-230.
http://dx.doi.org/10.1016/j.neuroscience.2011.06.021