Artículo
Kuhar, F.; Castiglia, V.C.; Zamora, J.C. "Detection of manganese peroxidase and other exoenzymes in four isolates of Geastrum (Geastrales) in pure culture " (2016) Revista Argentina de Microbiologia. 48(4):274-278
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Abstract:
Knowledge regarding the enzymatic machinery of fungi is decisive to understand their ecological role. The species of the genus Geastrum are known to grow extremely slowly in pure culture, which makes it difficult to evaluate physiological parameters such as enzyme activity. Qualitative assays were performed on isolates of four species of this genus, showing evidence of laccase, cellulase, pectinase, amylase and lipase activity and suggesting that a wide range of carbon sources can be exploited by these species. For the first time in this genus, quantitative assays verified manganese peroxidase activity (up to 0.6 mU/g) in 30-day old cultures, as well as laccase, β-glycosidase and β-xylosidase activities. © 2016 Asociación Argentina de Microbiología
Registro:
| Documento: | Artículo |
| Título: | Detection of manganese peroxidase and other exoenzymes in four isolates of Geastrum (Geastrales) in pure culture |
| Autor: | Kuhar, F.; Castiglia, V.C.; Zamora, J.C. |
| Filiación: | Centro de Investigación y Extensión Forestal Andino Patagónico – CONICET, Argentina Laboratorio de Micología Experimental, Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina Departamento de Micología, Real Jardín Botánico, CSIC, Madrid, Spain |
| Palabras clave: | Cellulase; Laccase; Lipase; Litter degradation; Xylanase; amino acid; amylase; cellulase; glycosidase; laccase; manganese peroxidase; nitrophenol; pectin; polygalacturonase; proton transporting adenosine triphosphate synthase; ribosome DNA; RNA polymerase II; triacylglycerol lipase; xenobiotic agent; xylan 1,4 beta xylosidase; xylan endo 1,3 beta xylosidase; amylase; carbon; cellulase; culture medium; fungal protein; laccase; manganese peroxidase; peroxidase; polygalacturonase; triacylglycerol lipase; agar medium; Article; Cantharellales; decolorization; enzyme activity; fungal cell culture; Geastrum; Geastrum argentinum; Geastrum morganii; Geastrum papinuttii; Geastrum plicatum; litter decomposition; mycelium; nonhuman; Basidiomycetes; comparative study; culture medium; enzymology; growth, development and aging; metabolism; species difference; Amylases; Basidiomycota; Carbon; Cellulase; Culture Media; Fungal Proteins; Laccase; Lipase; Peroxidases; Polygalacturonase; Species Specificity |
| Año: | 2016 |
| Volumen: | 48 |
| Número: | 4 |
| Página de inicio: | 274 |
| Página de fin: | 278 |
| DOI: | http://dx.doi.org/10.1016/j.ram.2016.09.002 |
| Título revista: | Revista Argentina de Microbiologia |
| Título revista abreviado: | Rev. Argent. Microbiol. |
| ISSN: | 03257541 |
| CODEN: | RAMID |
| CAS: | amino acid, 65072-01-7; amylase, 9000-90-2, 9000-92-4, 9001-19-8; cellulase, 9012-54-8; glycosidase, 9032-92-2; laccase, 80498-15-3; manganese peroxidase, 114995-15-2; nitrophenol, 25154-55-6; pectin, 9000-69-5; polygalacturonase, 9023-92-1, 9032-75-1; proton transporting adenosine triphosphate synthase, 37205-63-3; triacylglycerol lipase, 9001-62-1; xylan 1,4 beta xylosidase, 9025-53-0; xylan endo 1,3 beta xylosidase, 37278-89-0, 9025-55-2; carbon, 7440-44-0; peroxidase, 9003-99-0; Amylases; Carbon; Cellulase; Culture Media; Fungal Proteins; Laccase; Lipase; manganese peroxidase; Peroxidases; Polygalacturonase |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v48_n4_p274_Kuhar |
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Citas:
---------- APA ----------
Kuhar, F., Castiglia, V.C. & Zamora, J.C. (2016) . Detection of manganese peroxidase and other exoenzymes in four isolates of Geastrum (Geastrales) in pure culture . Revista Argentina de Microbiologia, 48(4), 274-278.http://dx.doi.org/10.1016/j.ram.2016.09.002
---------- CHICAGO ----------
Kuhar, F., Castiglia, V.C., Zamora, J.C. "Detection of manganese peroxidase and other exoenzymes in four isolates of Geastrum (Geastrales) in pure culture " . Revista Argentina de Microbiologia 48, no. 4 (2016) : 274-278.http://dx.doi.org/10.1016/j.ram.2016.09.002
---------- MLA ----------
Kuhar, F., Castiglia, V.C., Zamora, J.C. "Detection of manganese peroxidase and other exoenzymes in four isolates of Geastrum (Geastrales) in pure culture " . Revista Argentina de Microbiologia, vol. 48, no. 4, 2016, pp. 274-278.http://dx.doi.org/10.1016/j.ram.2016.09.002
---------- VANCOUVER ----------
Kuhar, F., Castiglia, V.C., Zamora, J.C. Detection of manganese peroxidase and other exoenzymes in four isolates of Geastrum (Geastrales) in pure culture . Rev. Argent. Microbiol. 2016;48(4):274-278.http://dx.doi.org/10.1016/j.ram.2016.09.002
