Artículo
Abstract:
Porphyrinogen carboxylyase from normal rat liver was subjected to purification methods. Two different purification protocols were used. In both cases, the initial steps consisted in obtaining a liver homogenate, followed by centrifugation, salt precipitation and phosphate gel absorption. Scheme I consisted in then submitted the preparation to DEAE-cellulose, followed by Sephacryl S-200 and Phenyl-sepharose sequential column chromatographies. Scheme II involved an affinity column followed by a Sephadex G-75 gel filtration column. In both cases, the enzyme was stored at -20 degrees C until its assay. The addition of 2mM dithiotreytol to the incubation media or to the enzyme extract before storage, did not help improve the activity nor the stability of the enzyme. Those fractions containing the maximal enzyme activity, detected using Uroporphyrinogen III or Pentacarboxy-porphyrinogen III as substrate, were not always present in the same tubes for the different columns employed. In addition, the degree of purification obtained in some steps was different according to the substrate employed. The results suggest the existence of at least two isoenzymes for rat liver porphyrinogen carboxy-lyase.
Registro:
| Documento: | Artículo |
| Título: | Porphyrinogen carboxylyase. Studies on the existence of isoenzymes. |
| Autor: | Ríos de Molina, M.C.; Chaufan, G.; Iglesias, S.; Billi de Catabbi, S.; San Martín de Viale, L.C. |
| Filiación: | Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina |
| Palabras clave: | isoenzyme; porphyrin; porphyrinogen; animal; article; comparative study; enzymology; isolation and purification; liver; metabolism; rat; Animals; Isoenzymes; Liver; Porphyrinogens; Porphyrins; Rats |
| Año: | 1996 |
| Volumen: | 46 |
| Número: | 4 |
| Página de inicio: | 265 |
| Página de fin: | 275 |
| Título revista: | Acta physiologica, pharmacologica et therapeutica latinoamericana : órgano de la Asociación Latinoamericana de Ciencias Fisiológicas y [de] la Asociación Latinoamericana de Farmacología |
| Título revista abreviado: | Acta Physiol Pharmacol Ther Latinoam |
| ISSN: | 03276309 |
| CAS: | porphyrin, 24869-67-8; porphyrinogen, 4396-11-6; Isoenzymes; Porphyrinogens; Porphyrins |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03276309_v46_n4_p265_RiosdeMolina |
Citas:
---------- APA ----------
Ríos de Molina, M.C., Chaufan, G., Iglesias, S., Billi de Catabbi, S. & San Martín de Viale, L.C. (1996) . Porphyrinogen carboxylyase. Studies on the existence of isoenzymes. Acta physiologica, pharmacologica et therapeutica latinoamericana : órgano de la Asociación Latinoamericana de Ciencias Fisiológicas y [de] la Asociación Latinoamericana de Farmacología, 46(4), 265-275.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03276309_v46_n4_p265_RiosdeMolina [ ]
---------- CHICAGO ----------
Ríos de Molina, M.C., Chaufan, G., Iglesias, S., Billi de Catabbi, S., San Martín de Viale, L.C. "Porphyrinogen carboxylyase. Studies on the existence of isoenzymes." Acta physiologica, pharmacologica et therapeutica latinoamericana : órgano de la Asociación Latinoamericana de Ciencias Fisiológicas y [de] la Asociación Latinoamericana de Farmacología 46, no. 4 (1996) : 265-275.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03276309_v46_n4_p265_RiosdeMolina [ ]
---------- MLA ----------
Ríos de Molina, M.C., Chaufan, G., Iglesias, S., Billi de Catabbi, S., San Martín de Viale, L.C. "Porphyrinogen carboxylyase. Studies on the existence of isoenzymes." Acta physiologica, pharmacologica et therapeutica latinoamericana : órgano de la Asociación Latinoamericana de Ciencias Fisiológicas y [de] la Asociación Latinoamericana de Farmacología, vol. 46, no. 4, 1996, pp. 265-275.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03276309_v46_n4_p265_RiosdeMolina [ ]
---------- VANCOUVER ----------
Ríos de Molina, M.C., Chaufan, G., Iglesias, S., Billi de Catabbi, S., San Martín de Viale, L.C. Porphyrinogen carboxylyase. Studies on the existence of isoenzymes. Acta Physiol Pharmacol Ther Latinoam. 1996;46(4):265-275.Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03276309_v46_n4_p265_RiosdeMolina [ ]
