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Abstract:

Neuropeptide Y (NPY) is considered the most potent orexigenic peptide, increasing before meal time and during fasting. In teleost, most studies on NPY action upon growth hormone (GH) and luteinizing hormone (LH) were conducted in females or group of animals without sex discrimination. The aim of this study was to evaluate whether NPY modulates the expression and release of GH and gonadotropins in both sexes of Cichlasoma dimerus. By double-label immunofluorescence, we first determined the association between NPY fibers and pituitary cells. In addition, we performed in vitro studies to evaluate the effect of NPY on GH and gonadotropins expression by real-time PCR, and release by Western blot, in males and females separately. Contacts between NPY fibers and GH and follicle-stimulating hormone (FSH)-producing cells were detected, indicating possible functional relationships. We observed an increase in GH release in the culture medium at 2 nM for males (p = 0.043) and 20 nM for females (p = 0.028). Pituitary FSH release was stimulated at 20 nM (p = 0.026) and 200 nM (p = 0.033) for males and females, respectively. Finally, NPY only increased β-LH mRNA expression at 20 nM in females (p = 0.028) and its release at 2 nM (p = 0.049) and 200 nM for males (p = 0.005) and 200 nM for females (p = 0.018). In conclusion, NPY acts as a GH-, LH- and FSH-releasing factor, in a dose- and sex-dependent way. © 2015, Springer Science+Business Media Dordrecht.

Registro:

Documento: Artículo
Título:Growth hormone, luteinizing hormone, and follicle-stimulating hormone regulation by neuropeptide Y in both sexes of the cichlid fish, Cichlasoma dimerus
Autor:Di Yorio, M.P.; Delgadin, T.H.; Pérez Sirkin, D.I.; Vissio, P.G.
Filiación:Laboratorio de Neuroendocrinología del Crecimiento y la Reproducción, DBBE, FCEN-UBA, Ciudad Universitaria, Buenos Aires, C1428EHA, Argentina
IBBEA-CONICET-UBA, Ciudad Universitaria, Buenos Aires, C1428EHA, Argentina
Palabras clave:Cichlids; FSH; GH; LH; NPY; Animalia; Cichlasoma; Cichlidae; Teleostei; follitropin; growth hormone; luteinizing hormone; neuropeptide Y; animal; cichlid; drug effects; female; genetics; hypophysis; male; metabolism; Animals; Cichlids; Female; Follicle Stimulating Hormone; Growth Hormone; Luteinizing Hormone; Male; Neuropeptide Y; Pituitary Gland
Año:2015
Volumen:41
Número:4
Página de inicio:843
Página de fin:852
DOI: http://dx.doi.org/10.1007/s10695-015-0051-z
Título revista:Fish Physiology and Biochemistry
Título revista abreviado:Fish Physiol. Biochem.
ISSN:09201742
CODEN:FPBIE
CAS:follitropin, 9002-68-0; growth hormone, 36992-73-1, 37267-05-3, 66419-50-9, 9002-72-6; luteinizing hormone, 39341-83-8, 9002-67-9; neuropeptide Y, 82785-45-3, 83589-17-7; Follicle Stimulating Hormone; Growth Hormone; Luteinizing Hormone; Neuropeptide Y
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09201742_v41_n4_p843_DiYorio

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Citas:

---------- APA ----------
Di Yorio, M.P., Delgadin, T.H., Pérez Sirkin, D.I. & Vissio, P.G. (2015) . Growth hormone, luteinizing hormone, and follicle-stimulating hormone regulation by neuropeptide Y in both sexes of the cichlid fish, Cichlasoma dimerus. Fish Physiology and Biochemistry, 41(4), 843-852.
http://dx.doi.org/10.1007/s10695-015-0051-z
---------- CHICAGO ----------
Di Yorio, M.P., Delgadin, T.H., Pérez Sirkin, D.I., Vissio, P.G. "Growth hormone, luteinizing hormone, and follicle-stimulating hormone regulation by neuropeptide Y in both sexes of the cichlid fish, Cichlasoma dimerus" . Fish Physiology and Biochemistry 41, no. 4 (2015) : 843-852.
http://dx.doi.org/10.1007/s10695-015-0051-z
---------- MLA ----------
Di Yorio, M.P., Delgadin, T.H., Pérez Sirkin, D.I., Vissio, P.G. "Growth hormone, luteinizing hormone, and follicle-stimulating hormone regulation by neuropeptide Y in both sexes of the cichlid fish, Cichlasoma dimerus" . Fish Physiology and Biochemistry, vol. 41, no. 4, 2015, pp. 843-852.
http://dx.doi.org/10.1007/s10695-015-0051-z
---------- VANCOUVER ----------
Di Yorio, M.P., Delgadin, T.H., Pérez Sirkin, D.I., Vissio, P.G. Growth hormone, luteinizing hormone, and follicle-stimulating hormone regulation by neuropeptide Y in both sexes of the cichlid fish, Cichlasoma dimerus. Fish Physiol. Biochem. 2015;41(4):843-852.
http://dx.doi.org/10.1007/s10695-015-0051-z