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Abstract:

The aim of the present work was to determine the in vitro effect of Mentha piperita and Mentha pulegium essential oils against Echinococcus granulosus and to compare the effectiveness of both oils according to the exposure time and concentration. Although both treatments had a protoscolicidal effect, M. pulegium had a considerably stronger effect than M. piperita. Essential oil of M. pulegium produced dose- and time-dependent effects. Maximal protoscolicidal effect was observed after 12 days of incubation and reached 0% after 18 days. This lack of viability was proved during the determination of infectivity into mice. Essential oil of M. piperita produced only a time-dependent effect. At 24 days p.i., the viability of protoscoleces decreased to approximately 50%. Scanning and transmission electron microscopy (SEM and TEM) demonstrated the drug-induced ultrastructural damage. On the other hand, a loss of turgidity was detected in all M. pulegium-treated cysts respective of the drug concentration. There was a correlation between the intensity of damage and the concentration of the essential oil assayed. Studies by SEM revealed that the germinal layer of treated cysts lost the feature multicellular structure. M. pulegium essential oil showed piperitone oxide as main compound in their composition, and we suggest that this component could be responsible of the markedly anthelmintic effect detected. Our data suggest that essential oils of Mentha spp. can be a promising source of potential protoscolicidal agents. The isolation of active anthelmintic constituents is in progress and may lead to the discovery of compounds with improved therapeutic value. © Springer-Verlag 2011.

Registro:

Documento: Artículo
Título:Anthelmintic effect of Mentha spp. essential oils on Echinococcus granulosus protoscoleces and metacestodes
Autor:Maggiore, M.A.; Albanese, A.A.; Gende, L.B.; Eguaras, M.J.; Denegri, G.M.; Elissondo, M.C.
Filiación:Laboratorio de Zoonosis Parasitarias, Departamento de Biología, Universidad Nacional de Mar del Plata (UNMdP), Funes 3350, 7600 Mar del Plata, Argentina
Laboratorio de Artrópodos, Departamento de Biología, Universidad Nacional de Mar del Plata (UNMdP), Funes 3350, 7600 Mar del Plata, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina
Laboratorio de Zoonosis Parasitarias, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata (UNMdP), Funes 3350, 7600 Mar del Plata, Argentina
Idioma: Inglés
Palabras clave:anthelmintic agent; essential oil; vegetable oil; Mentha piperita essential oil; Mentha pulegium essential oil; unclassified drug; animal; article; chemistry; comparative study; dose response; drug effect; drug sensitivity; echinococcosis; Echinococcus granulosus; female; growth, development and aging; Mentha piperita; Mentha pulegium; mouse; parasitology; pathogenicity; phytotherapy; scanning electron microscopy; species difference; survival; time; transmission electron microscopy; ultrastructure; cestode life cycle stage; concentration response; controlled study; cyst (resting stage); in vitro study; metacestode; nonhuman; parasite survival; physical chemistry; plant leaf; priority journal; protoscole; Animals; Anthelmintics; Dose-Response Relationship, Drug; Echinococcosis; Echinococcus granulosus; Female; Mentha piperita; Mentha pulegium; Mice; Microscopy, Electron, Scanning; Microscopy, Electron, Transmission; Oils, Volatile; Parasitic Sensitivity Tests; Phytotherapy; Plant Oils; Species Specificity; Survival Analysis; Time Factors; Echinococcus granulosus; Mentha; Mentha pulegium; Mentha x piperita; Mus
Año:2012
Volumen:110
Número:3
Página de inicio:1103
Página de fin:1112
DOI: http://dx.doi.org/10.1007/s00436-011-2595-x
Título revista:Parasitology Research
Título revista abreviado:Parasitol. Res.
ISSN:09320113
CODEN:PARRE
CAS:Anthelmintics; Oils, Volatile; Plant Oils
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09320113_v110_n3_p1103_Maggiore

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Citas:

---------- APA ----------
Maggiore, M.A., Albanese, A.A., Gende, L.B., Eguaras, M.J., Denegri, G.M. & Elissondo, M.C. (2012) . Anthelmintic effect of Mentha spp. essential oils on Echinococcus granulosus protoscoleces and metacestodes. Parasitology Research, 110(3), 1103-1112.
http://dx.doi.org/10.1007/s00436-011-2595-x
---------- CHICAGO ----------
Maggiore, M.A., Albanese, A.A., Gende, L.B., Eguaras, M.J., Denegri, G.M., Elissondo, M.C. "Anthelmintic effect of Mentha spp. essential oils on Echinococcus granulosus protoscoleces and metacestodes" . Parasitology Research 110, no. 3 (2012) : 1103-1112.
http://dx.doi.org/10.1007/s00436-011-2595-x
---------- MLA ----------
Maggiore, M.A., Albanese, A.A., Gende, L.B., Eguaras, M.J., Denegri, G.M., Elissondo, M.C. "Anthelmintic effect of Mentha spp. essential oils on Echinococcus granulosus protoscoleces and metacestodes" . Parasitology Research, vol. 110, no. 3, 2012, pp. 1103-1112.
http://dx.doi.org/10.1007/s00436-011-2595-x
---------- VANCOUVER ----------
Maggiore, M.A., Albanese, A.A., Gende, L.B., Eguaras, M.J., Denegri, G.M., Elissondo, M.C. Anthelmintic effect of Mentha spp. essential oils on Echinococcus granulosus protoscoleces and metacestodes. Parasitol. Res. 2012;110(3):1103-1112.
http://dx.doi.org/10.1007/s00436-011-2595-x