Artículo
Fumagalli, S.A.; Kotler, M.L.; Rossetti, M.V.; Batlle, A.M.D.C. "Studies on Uroporphyrinogen Biosynthesis in Pig Liver" (1991) Zeitschrift fur Naturforschung - Section C Journal of Biosciences. 46(11-12):1101-1108
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Abstract:
Porphobilinogen-deaminase (PBG-D) and PBG-D-isomerase complex (PBG-D-I) from pig liver were isolated and partially purified. Uroporphyrinogen I and III formation was found to be linear with time and protein concentration. Optimal pH was about 7.4 and 7.6-7.8 for PBG-D and PBG-D-I complex, respectively. Some properties of the isolated enzymes were studied. Molecular mass determination gave a value of 40,000 Da for PBG-D and 50,000 Da for the complex. Both enzymes exhibited classical Michaelis-Menten kinetics. Km and Vmax parameters were estimated. The effect of several divalent cations, ammonia and thiol reagents was also investigated. The differential action of some of these chemicals on PBG-D and PBG-D-I system would suggest that PBG-D and isomerase may not be only physically adjacent but actually associated. © 1991, Verlag der Zeitschrift für Naturforschung. All rights reserved.
Registro:
| Documento: | Artículo |
| Título: | Studies on Uroporphyrinogen Biosynthesis in Pig Liver |
| Autor: | Fumagalli, S.A.; Kotler, M.L.; Rossetti, M.V.; Batlle, A.M.D.C. |
| Filiación: | Centro de Investigations sobre Porfirinas y Porfirias - CIPYP (CONICET; F.C.E. y N., UBA)-Ciudad Universitaria, Pabellón II, 2do piso, 1428 Buenos Aires, Argentina |
| Palabras clave: | Association; PBG-D-I Complex; PBG-Deaminase; Pig Liver; Uroporphyrins; ammonia; cation; multienzyme complex; porphobilinogen deaminase; thiol reagent; uroporphyrinogen; uroporphyrinogen III synthase; animal; article; biosynthesis; enzymology; gel chromatography; isolation and purification; kinetics; liver; metabolism; molecular weight; swine; Ammonia; Animal; Cations; Chromatography, Gel; Hydroxymethylbilane Synthase; Kinetics; Liver; Molecular Weight; Multienzyme Complexes; Sulfhydryl Reagents; Support, Non-U.S. Gov't; Swine; Uroporphyrinogen-III Synthase; Uroporphyrinogens |
| Año: | 1991 |
| Volumen: | 46 |
| Número: | 11-12 |
| Página de inicio: | 1101 |
| Página de fin: | 1108 |
| DOI: | http://dx.doi.org/10.1515/znc-1991-11-1228 |
| Título revista: | Zeitschrift fur Naturforschung - Section C Journal of Biosciences |
| Título revista abreviado: | Z. Naturforsch. Sect. C J. Biosci. |
| ISSN: | 09395075 |
| CAS: | ammonia, 14798-03-9, 51847-23-5, 7664-41-7; porphobilinogen deaminase, 9036-47-9, 9074-91-3; uroporphyrinogen III synthase, 37340-55-9; uroporphyrinogen, 35465-57-7; Ammonia, 7664-41-7; Cations; Hydroxymethylbilane Synthase, EC 4.3.1.8; Multienzyme Complexes; Sulfhydryl Reagents; Uroporphyrinogen-III Synthase, EC 4.2.1.75; Uroporphyrinogens |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09395075_v46_n11-12_p1101_Fumagalli |
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Citas:
---------- APA ----------
Fumagalli, S.A., Kotler, M.L., Rossetti, M.V. & Batlle, A.M.D.C. (1991) . Studies on Uroporphyrinogen Biosynthesis in Pig Liver. Zeitschrift fur Naturforschung - Section C Journal of Biosciences, 46(11-12), 1101-1108.http://dx.doi.org/10.1515/znc-1991-11-1228
---------- CHICAGO ----------
Fumagalli, S.A., Kotler, M.L., Rossetti, M.V., Batlle, A.M.D.C. "Studies on Uroporphyrinogen Biosynthesis in Pig Liver" . Zeitschrift fur Naturforschung - Section C Journal of Biosciences 46, no. 11-12 (1991) : 1101-1108.http://dx.doi.org/10.1515/znc-1991-11-1228
---------- MLA ----------
Fumagalli, S.A., Kotler, M.L., Rossetti, M.V., Batlle, A.M.D.C. "Studies on Uroporphyrinogen Biosynthesis in Pig Liver" . Zeitschrift fur Naturforschung - Section C Journal of Biosciences, vol. 46, no. 11-12, 1991, pp. 1101-1108.http://dx.doi.org/10.1515/znc-1991-11-1228
---------- VANCOUVER ----------
Fumagalli, S.A., Kotler, M.L., Rossetti, M.V., Batlle, A.M.D.C. Studies on Uroporphyrinogen Biosynthesis in Pig Liver. Z. Naturforsch. Sect. C J. Biosci. 1991;46(11-12):1101-1108.http://dx.doi.org/10.1515/znc-1991-11-1228
