Artículo
Abstract:
This report demonstrates the ability of folic acid to activate rat liver porphobilinogen-deaminase (PBG-D). Lineweaver-Burk analysis revealed an increase in Vmax (38%) without affecting the Km. In the concentration range assayed, secondary replots of 1/Aslope and 1/Aintersect versus 1/[folic acid] yielded straight lines, indicating the binding of a single molecule of activator to the enzyme PBG-D, with a KA = 1.66 mM. Results presented here show that folic acid acts as a non-essential activator (α = 1; ß= 1.6). The activating effect of folic acid has been observed employing the 35-70% ammonium sulphate precipitated fraction, desalted by dialysis or gel filtration, whereas no action was detected when other partially purified PBG-D preparations were utilized as the enzyme source, suggesting either the presence of sites saturated for the activator, or the existence of a different structural protein conformation, or both. © 1992, Walter de Gruyter. All rights reserved.
Registro:
| Documento: | Artículo |
| Título: | Non-Essential Activation of Rat Liver Porphobilinogen-Deaminase by Folic Acid* |
| Autor: | Noriega, G.O.; Juknat, A.A.; Alcira, M.; del Batlle, C. |
| Filiación: | Centro de Investigaciones sobre Porfirinas y Porfirias (CIPYP), Department of Biochemistry, University of Buenos Aires, Ciudad Universitaria, Pabellon II, 1428 Buenos Aires, Argentine, Argentina |
| Palabras clave: | Cosynthetase; Folic Acid; Nonessential Activation; Porphobilinogen-Deaminase; Uroporphyrinogen I; folic acid; porphobilinogen deaminase; animal; article; cell fractionation; cytosol; enzyme activation; enzymology; isolation and purification; kinetics; liver; male; metabolism; protein binding; rat; rat strain; theoretical model; Animal; Cell Fractionation; Cytosol; Enzyme Activation; Folic Acid; Hydroxymethylbilane Synthase; Kinetics; Liver; Male; Models, Theoretical; Protein Binding; Rats; Rats, Inbred Strains; Support, Non-U.S. Gov't |
| Año: | 1992 |
| Volumen: | 47 |
| Número: | 5-6 |
| Página de inicio: | 416 |
| Página de fin: | 419 |
| DOI: | http://dx.doi.org/10.1515/znc-1992-0616 |
| Título revista: | Zeitschrift fur Naturforschung - Section C Journal of Biosciences |
| Título revista abreviado: | Z. Naturforsch. Sect. C J. Biosci. |
| ISSN: | 09395075 |
| CAS: | folic acid, 59-30-3, 6484-89-5; porphobilinogen deaminase, 9036-47-9, 9074-91-3; Folic Acid, 59-30-3; Hydroxymethylbilane Synthase, EC 4.3.1.8 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09395075_v47_n5-6_p416_Noriega |
Referencias:
- G. J. Hart and A. R. Battersby, Biochem. J. 232, 151-160 (1985). ; E. Smythe and D. C. Williams, Biochem. J. 253, 275-279 (1988). ; A. A. Juknat, M. V. Rossetti, and A. M. del C. Batlle, Int. J. Biochem. 13, 343–353 (1981). ; A. A. Juknat, D. Doernemann, and H. Senger, Z. Naturforsch. 43c, 351-356 (1988). ; A. A. Juknat, D. Doernemann, and H. Senger, Z. Naturforsch. 43c, 357–362 (1988). ; W. R. Christenson, V. R. Reddy, and W. N. Piper; Life Sci. 38, 1679 -1684 (1986). ; M. L. Kotler, A. A. Juknat, S. R. Correa Garcia, F. G. Princ, and A. M. del C. Batlle, Med. Sci. Res. 16, 983-984 (1988). ; Kotler, M.L., Juknat, A.A., Correa Garcia, S.R., Princ, F.G., (1988) Res and A. M. del C. Batlle, Med. Sci., 16, pp. 983-984
Citas:
---------- APA ----------
Noriega, G.O., Juknat, A.A., Alcira, M. & del Batlle, C. (1992) . Non-Essential Activation of Rat Liver Porphobilinogen-Deaminase by Folic Acid*. Zeitschrift fur Naturforschung - Section C Journal of Biosciences, 47(5-6), 416-419.http://dx.doi.org/10.1515/znc-1992-0616
---------- CHICAGO ----------
Noriega, G.O., Juknat, A.A., Alcira, M., del Batlle, C. "Non-Essential Activation of Rat Liver Porphobilinogen-Deaminase by Folic Acid*" . Zeitschrift fur Naturforschung - Section C Journal of Biosciences 47, no. 5-6 (1992) : 416-419.http://dx.doi.org/10.1515/znc-1992-0616
---------- MLA ----------
Noriega, G.O., Juknat, A.A., Alcira, M., del Batlle, C. "Non-Essential Activation of Rat Liver Porphobilinogen-Deaminase by Folic Acid*" . Zeitschrift fur Naturforschung - Section C Journal of Biosciences, vol. 47, no. 5-6, 1992, pp. 416-419.http://dx.doi.org/10.1515/znc-1992-0616
---------- VANCOUVER ----------
Noriega, G.O., Juknat, A.A., Alcira, M., del Batlle, C. Non-Essential Activation of Rat Liver Porphobilinogen-Deaminase by Folic Acid*. Z. Naturforsch. Sect. C J. Biosci. 1992;47(5-6):416-419.http://dx.doi.org/10.1515/znc-1992-0616
