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Abstract:

The trans-sialidase from the trypomastigote stage of Trypanosoma cruzi was metabolically labeled with [3H]-palmitic acid and purified by immunoprecipitation with a monoclonal antibody. The action of PI-PLC on the immunoprecipitate released a lipid that was analyzed by TLC. Lyso-1-O-h exadecylglycerol and N-palmitoyl-sphinganine were obtained in a 1:3 ratio. A comparison with the GPI anchors present in the different stages of T. ruzi was made.

Registro:

Documento: Artículo
Título:The trans-sialidase of Trypanosoma cruzi is anchored by two different lipids
Autor:Agusti, R.; Couto, A.S.; Campetella, O.E.; Frasch, A.C.C.; De Lederkremer, R.M.
Filiación:CIHIDECAR, Depto. de Quim. Orgánica, Ciudad Universitaria, Pabellón 2, (1428) Buenos Aires, Argentina
Inst. Invest. Bioquimicas Fund. C., Antonio Machado 151, (1405) Buenos Aires, Argentina
Palabras clave:GPI; Trans-sialidase; Trypanosoma cruzi; lipid; palmitic acid; sialidase; article; immunoprecipitation; nonhuman; priority journal; thin layer chromatography; trypanosoma cruzi; Animals; Chromatography, Thin Layer; Glycosylphosphatidylinositols; Membrane Lipids; Mice; Neuraminidase; Phosphatidylinositol Diacylglycerol-Lyase; Phospholipase C; Substrate Specificity; Trypanosoma cruzi; Trypanosoma; Trypanosoma cruzi
Año:1997
Volumen:7
Número:6
Página de inicio:731
Página de fin:735
DOI: http://dx.doi.org/10.1093/glycob/7.6.731
Título revista:Glycobiology
Título revista abreviado:GLYCOBIOLOGY
ISSN:09596658
CODEN:GLYCE
CAS:Glycosylphosphatidylinositols; Membrane Lipids; Neuraminidase, EC 3.2.1.18; Phosphatidylinositol Diacylglycerol-Lyase, EC 4.6.1.13; Phospholipase C, EC 3.1.4.3
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09596658_v7_n6_p731_Agusti

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Citas:

---------- APA ----------
Agusti, R., Couto, A.S., Campetella, O.E., Frasch, A.C.C. & De Lederkremer, R.M. (1997) . The trans-sialidase of Trypanosoma cruzi is anchored by two different lipids. Glycobiology, 7(6), 731-735.
http://dx.doi.org/10.1093/glycob/7.6.731
---------- CHICAGO ----------
Agusti, R., Couto, A.S., Campetella, O.E., Frasch, A.C.C., De Lederkremer, R.M. "The trans-sialidase of Trypanosoma cruzi is anchored by two different lipids" . Glycobiology 7, no. 6 (1997) : 731-735.
http://dx.doi.org/10.1093/glycob/7.6.731
---------- MLA ----------
Agusti, R., Couto, A.S., Campetella, O.E., Frasch, A.C.C., De Lederkremer, R.M. "The trans-sialidase of Trypanosoma cruzi is anchored by two different lipids" . Glycobiology, vol. 7, no. 6, 1997, pp. 731-735.
http://dx.doi.org/10.1093/glycob/7.6.731
---------- VANCOUVER ----------
Agusti, R., Couto, A.S., Campetella, O.E., Frasch, A.C.C., De Lederkremer, R.M. The trans-sialidase of Trypanosoma cruzi is anchored by two different lipids. GLYCOBIOLOGY. 1997;7(6):731-735.
http://dx.doi.org/10.1093/glycob/7.6.731