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Abstract:

During the immune response, a great number of cytokines that modulate the function of mononuclear phagocytes are produced. Since interferons are one of the most important cytokines, the aim of this study was to evaluate the expression of HLA-DR antigen after an 18-h culture with human recombinant interferon-γ (hrIFN-γ) on freshly isolated peripheral blood monocytes from 16 colorectal cancer patients and 16 healthy donors, using an indirect immunofluorescence method. The results obtained showed that there was a decreased percentage of HLA-DR+ monocytes in the colorectal cancer patents (51 ± 3%, p < 0.01) compared with the healthy donors (77 ± 2%). Treatment for 18 h with hrIFN-γ increased the percentages of monocytes expressing HLA-DR: 71 ± 3% for the cancer patients and 84 ± 2% for the healthy donors (p < 0.01 and < 0.001, respectively). Our results demonstrate that after in vitro treatment with hrIFN-γ, functionally altered monocytes from colorectal cancer patients can reach major histocompatibility complex II antigen expression values similar to those of healthy donors, thus improving the host's cellular immune response against the tumor.

Registro:

Documento: Artículo
Título:Regulation of HLA-DR antigen in monocytes from colorectal cancer patients by in vitro treatment with human recombinant interferon-γ
Autor:Novellino, P.S.; Trejo, Y.G.; Beviacqua, M.; Bordenave, R.H.; Rumi, L.S.
Filiación:Immunology Laboratory, Inst. de Biol. y Med. Exp. (IByME), Buenos Aires, Argentina
Hospital de Agudos Iriarte, Buenos Aires, Argentina
Inst. de Biol. y Med. Experimental, Vuelta de Obligado 2490, (1428) Buenos Aires, Argentina
Palabras clave:Colorectal cancer; HLA-DR; Interferon-γ; Monocytes; cytokine; HLA DR antigen; major histocompatibility antigen class 2; recombinant gamma interferon; article; cellular immunity; clinical article; colorectal cancer; controlled study; cytokine production; human; human cell; immune response; immunofluorescence; leukocyte culture; monocyte; mononuclear phagocyte; tumor immunity; Adenocarcinoma; Antigens, Neoplasm; Colorectal Neoplasms; Fluorescent Antibody Technique, Indirect; Gene Expression Regulation, Neoplastic; HLA-DR Antigens; Humans; Interferon-gamma, Recombinant; Leukocytes, Mononuclear
Año:2000
Volumen:10
Número:2
Página de inicio:90
Página de fin:93
Título revista:Journal of Investigational Allergology and Clinical Immunology
Título revista abreviado:J. Invest. Allergol. Clin. Immunol.
ISSN:10189068
CODEN:JIAIE
CAS:Antigens, Neoplasm; HLA-DR Antigens; Interferon-gamma, Recombinant
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10189068_v10_n2_p90_Novellino

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Citas:

---------- APA ----------
Novellino, P.S., Trejo, Y.G., Beviacqua, M., Bordenave, R.H. & Rumi, L.S. (2000) . Regulation of HLA-DR antigen in monocytes from colorectal cancer patients by in vitro treatment with human recombinant interferon-γ. Journal of Investigational Allergology and Clinical Immunology, 10(2), 90-93.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10189068_v10_n2_p90_Novellino [ ]
---------- CHICAGO ----------
Novellino, P.S., Trejo, Y.G., Beviacqua, M., Bordenave, R.H., Rumi, L.S. "Regulation of HLA-DR antigen in monocytes from colorectal cancer patients by in vitro treatment with human recombinant interferon-γ" . Journal of Investigational Allergology and Clinical Immunology 10, no. 2 (2000) : 90-93.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10189068_v10_n2_p90_Novellino [ ]
---------- MLA ----------
Novellino, P.S., Trejo, Y.G., Beviacqua, M., Bordenave, R.H., Rumi, L.S. "Regulation of HLA-DR antigen in monocytes from colorectal cancer patients by in vitro treatment with human recombinant interferon-γ" . Journal of Investigational Allergology and Clinical Immunology, vol. 10, no. 2, 2000, pp. 90-93.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10189068_v10_n2_p90_Novellino [ ]
---------- VANCOUVER ----------
Novellino, P.S., Trejo, Y.G., Beviacqua, M., Bordenave, R.H., Rumi, L.S. Regulation of HLA-DR antigen in monocytes from colorectal cancer patients by in vitro treatment with human recombinant interferon-γ. J. Invest. Allergol. Clin. Immunol. 2000;10(2):90-93.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10189068_v10_n2_p90_Novellino [ ]