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Abstract:

Apoptosis is the biological process by which follicular cells are eliminated in atretic follicles. The aim of the present study was to examine the in vitro effect of a GnRH-a (leuprolide acetate, LA) and its interactions with FSH, dibutyryl cAMP, and growth factors (IGF-I, EGF, and FGF) on follicular apoptosis in early antral ovarian follicles obtained from prepubertal DES-treated rats. Follicles cultured 24 hr in the absence of hormones showed spontaneous onset of apoptotic DNA fragmentation. The presence of FSH suppressed the spontaneous onset of apoptotic DNA fragmentation (75-85%). Quantitative estimation of DNA cleavage from ovarian follicles revealed no significant changes in DNA fragmentation after in vitro LA treatment (1-100 ng/ml). However, coincubation with LA interfered partially with the effects of FSH on apoptosis suppression. This apoptosis suppression was also obtained by treatment with dibutyryl cAMP (80%), and was partially prevented by the presence of LA in the cultures. Follicles were cultured 24 hr with FGF, EGF, or IGF-I, and these factors suppressed DNA fragmentation (70, 60, and 70% respectively), while the presence of LA (100 ng/ml) in the culture medium prevented this effect. In conclusion, we show that the rescue from apoptotic DNA fragmentation produced in early antral follicles by FSH, cAMP, and growth factors, is prevented by coincubation with LA. This GnRH analog would thus interfere in the pathway of FSH, cAMP and/or growth factors by an as yet unknown mechanism. © 2001 Wiley-Liss, Inc.

Registro:

Documento: Artículo
Título:Gonadotropin-releasing hormone agonist affects rat ovarian follicle development by interfering with FSH and growth factors on the prevention of apoptosis
Autor:Parborell, F.; Dain, L.; Tesone, M.
Filiación:Instituto de Biologia y Medicina Experimental (IBYME) CONICET, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
Instituto de Biología y Medicina Experimental, Obligado 2490, (1428) Buenos Aires, Argentina
Palabras clave:Apoptosis; Early antral follicles; GnRH; Growth factors; Ovary; diethylstilbestrol; DNA fragment; follitropin; gonadorelin agonist; growth factor; leuprorelin; animal cell; apoptosis; article; cell culture; controlled study; DNA cleavage; female; in vitro study; nonhuman; ovary development; ovary follicle; prepuberty; priority journal; rat; Animals; Apoptosis; Bucladesine; Cells, Cultured; Culture Media, Serum-Free; Diethylstilbestrol; DNA Fragmentation; Epidermal Growth Factor; Estrogens, Non-Steroidal; Female; Fertility Agents, Female; Fibroblast Growth Factors; Follicle Stimulating Hormone; Follicular Atresia; Gonadotropin-Releasing Hormone; Insulin-Like Growth Factor I; Leuprolide; Ovarian Follicle; Rats; Rats, Sprague-Dawley; Sexual Maturation
Año:2001
Volumen:60
Número:2
Página de inicio:241
Página de fin:247
DOI: http://dx.doi.org/10.1002/mrd.1084
Título revista:Molecular Reproduction and Development
Título revista abreviado:Mol. Reprod. Dev.
ISSN:1040452X
CODEN:MREDE
CAS:Bucladesine, 362-74-3; Culture Media, Serum-Free; Diethylstilbestrol, 56-53-1; Epidermal Growth Factor, 62229-50-9; Estrogens, Non-Steroidal; Fertility Agents, Female; Fibroblast Growth Factors, 62031-54-3; Follicle Stimulating Hormone, 9002-68-0; Gonadotropin-Releasing Hormone, 33515-09-2; Insulin-Like Growth Factor I, 67763-96-6; Leuprolide, 53714-56-0
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1040452X_v60_n2_p241_Parborell

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Citas:

---------- APA ----------
Parborell, F., Dain, L. & Tesone, M. (2001) . Gonadotropin-releasing hormone agonist affects rat ovarian follicle development by interfering with FSH and growth factors on the prevention of apoptosis. Molecular Reproduction and Development, 60(2), 241-247.
http://dx.doi.org/10.1002/mrd.1084
---------- CHICAGO ----------
Parborell, F., Dain, L., Tesone, M. "Gonadotropin-releasing hormone agonist affects rat ovarian follicle development by interfering with FSH and growth factors on the prevention of apoptosis" . Molecular Reproduction and Development 60, no. 2 (2001) : 241-247.
http://dx.doi.org/10.1002/mrd.1084
---------- MLA ----------
Parborell, F., Dain, L., Tesone, M. "Gonadotropin-releasing hormone agonist affects rat ovarian follicle development by interfering with FSH and growth factors on the prevention of apoptosis" . Molecular Reproduction and Development, vol. 60, no. 2, 2001, pp. 241-247.
http://dx.doi.org/10.1002/mrd.1084
---------- VANCOUVER ----------
Parborell, F., Dain, L., Tesone, M. Gonadotropin-releasing hormone agonist affects rat ovarian follicle development by interfering with FSH and growth factors on the prevention of apoptosis. Mol. Reprod. Dev. 2001;60(2):241-247.
http://dx.doi.org/10.1002/mrd.1084