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Abstract:

Alternative splicing plays a key role in generating protein diversity. Transfections with minigenes revealed coordination between two distant, alternatively spliced exons in the same gene. Mutations that either inhibit or stimulate inclusion of the upstream alternative exon deeply affect inclusion of the downstream one. However, similar mutations at the downstream alternative exon have little effect on the upstream one. This polar effect is promoter specific and is enhanced by inhibition of transcriptional elongation. Consistently, cells from mutant mice with either constitutive or null inclusion of a fibronectin alternative exon revealed coordination with a second alternative splicing region, located far downstream. Using allele-specific RT-PCR, we demonstrate that this coordination occurs in cis and is also affected by transcriptional elongation rates. Bioinformatics supports the generality of these findings, indicating that 25% of human genes contain multiple alternative splicing regions and identifying several genes with nonrandom distribution of mRNA isoforms at two alternative regions. Copyright ©2005 by Elsevier Inc.

Registro:

Documento: Artículo
Título:A polar mechanism coordinates different regions of alternative splicing within a single gene
Autor:Fededa, J.P.; Petrillo, E.; Gelfand, M.S.; Neverov, A.D.; Kadener, S.; Nogués, G.; Pelisch, F.; Baralle, F.E.; Muro, A.F.; Kornblihtt, A.R.
Filiación:Laboratorio de Fisiología Y Biología Molecular, Departamento de Fisiología, Biología Molecular Y Celular, Pabellón 2, (C1428EHA) Buenos Aires, Argentina
Institute for Information Transmission Problems, Russian Academy of Sciences, Bolshoi Karetny pereulok 19, Moscow 127994, Russian Federation
International Centre for Genetic Engineering and Biotechnology, Padriciano 99, 34012 Trieste, Italy
State Research Center GosNIIGenetika, Pervy Dorozhny proezd 1, Moscow 117545, Russian Federation
Palabras clave:fibronectin; alternative RNA splicing; article; bioinformatics; cis isomer; gene identification; human; nonhuman; transcription regulation; Alleles; Alpha-Globulins; Alternative Splicing; Animals; Antigens, Viral, Tumor; Cell Line, Tumor; Cercopithecus aethiops; Computational Biology; COS Cells; Dichlororibofuranosylbenzimidazole; DNA-Binding Proteins; Exons; Fibroblasts; Fibronectins; Genes; Humans; Mice; Mice, Knockout; Models, Genetic; Nuclear Proteins; Promoter Regions (Genetics); Protein Isoforms; RNA Polymerase II; RNA Splicing; RNA-Binding Proteins; Transcription Factors; Transfection
Año:2005
Volumen:19
Número:3
Página de inicio:393
Página de fin:404
DOI: http://dx.doi.org/10.1016/j.molcel.2005.06.035
Título revista:Molecular Cell
Título revista abreviado:Mol. Cell
ISSN:10972765
CODEN:MOCEF
CAS:fibronectin, 86088-83-7; Alpha-Globulins; Antigens, Viral, Tumor; Dichlororibofuranosylbenzimidazole, 53-85-0; DNA-Binding Proteins; Fibronectins; Nuclear Proteins; Protein Isoforms; RNA Polymerase II, EC 2.7.7.-; RNA-Binding Proteins; SFRS1 protein, human; Transcription Factors
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_10972765_v19_n3_p393_Fededa.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10972765_v19_n3_p393_Fededa

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Citas:

---------- APA ----------
Fededa, J.P., Petrillo, E., Gelfand, M.S., Neverov, A.D., Kadener, S., Nogués, G., Pelisch, F.,..., Kornblihtt, A.R. (2005) . A polar mechanism coordinates different regions of alternative splicing within a single gene. Molecular Cell, 19(3), 393-404.
http://dx.doi.org/10.1016/j.molcel.2005.06.035
---------- CHICAGO ----------
Fededa, J.P., Petrillo, E., Gelfand, M.S., Neverov, A.D., Kadener, S., Nogués, G., et al. "A polar mechanism coordinates different regions of alternative splicing within a single gene" . Molecular Cell 19, no. 3 (2005) : 393-404.
http://dx.doi.org/10.1016/j.molcel.2005.06.035
---------- MLA ----------
Fededa, J.P., Petrillo, E., Gelfand, M.S., Neverov, A.D., Kadener, S., Nogués, G., et al. "A polar mechanism coordinates different regions of alternative splicing within a single gene" . Molecular Cell, vol. 19, no. 3, 2005, pp. 393-404.
http://dx.doi.org/10.1016/j.molcel.2005.06.035
---------- VANCOUVER ----------
Fededa, J.P., Petrillo, E., Gelfand, M.S., Neverov, A.D., Kadener, S., Nogués, G., et al. A polar mechanism coordinates different regions of alternative splicing within a single gene. Mol. Cell. 2005;19(3):393-404.
http://dx.doi.org/10.1016/j.molcel.2005.06.035