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Abstract:

Transcription and pre-mRNA splicing are extremely complex multimolecular processes that involve protein-DNA, protein-RNA, and protein-protein interactions. Splicing occurs in the close vicinity of genes and is frequently cotranscriptional. This is consistent with evidence that both processes are coordinated and, in some cases, functionally coupled. This review focuses on the roles of cis- and trans-acting factors that regulate transcription, on constitutive and alternative splicing. We also discuss possible functions in splicing of the C-terminal domain (CTD) of the RNA polymerase II (pol II) largest subunit, whose participation in other key pre-mRNA processing reactions (capping and cleavage/polyadenylation) is well documented. Recent evidence indicates that transcriptional elongation and splicing can be influenced reciprocally: Elongation rates control alternative splicing and splicing factors can, in turn, modulate pol II elongation. The presence of transcription factors in the spliceosome and the existence of proteins, such as the coactivator PGC-1, with dual activities in splicing and transcription can explain the links between both processes and add a new level of complexity to the regulation of gene expression in eukaryotes.

Registro:

Documento: Artículo
Título:Multiple links between transcription and splicing
Autor:Kornblihtt, A.R.; De La Mata, M.; Fededa, J.P.; Muñoz, M.J.; Nogués, G.
Filiación:Depto. de Fisiol., Biol. Molec./Cel., Universidad de Buenos Aires, IFIByNE-CONICET, Buenos Aires, Argentina
Palabras clave:Alternative splicing; Coupling; RNA polymerase II; Splicing; Transcription; cis acting element; messenger RNA; PGC 1 protein; protein; RNA polymerase II; trans acting factor; transcription factor; unclassified drug; alternative RNA splicing; c terminal domain; eukaryote; gene; gene expression; gene expression regulation; minigene; polyadenylation; priority journal; protein DNA interaction; protein domain; protein protein interaction; protein RNA binding; review; RNA capping; RNA cleavage; rna polyadenylation; RNA processing; RNA splicing; RNA transcription; RNA translation; spliceosome; transcription elongation; transcription initiation; transcription regulation; Alternative Splicing; Animals; Humans; Models, Biological; Protein Subunits; RNA Polymerase II; RNA Precursors; RNA Splicing; Spliceosomes; Trans-Activators; Trans-Splicing; Transcription, Genetic; Eukaryota
Año:2004
Volumen:10
Número:10
Página de inicio:1489
Página de fin:1498
DOI: http://dx.doi.org/10.1261/rna.7100104
Título revista:RNA
Título revista abreviado:RNA
ISSN:13558382
CODEN:RNARF
CAS:protein, 67254-75-5; Protein Subunits; RNA Polymerase II, EC 2.7.7.-; RNA Precursors; Trans-Activators
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13558382_v10_n10_p1489_Kornblihtt

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Citas:

---------- APA ----------
Kornblihtt, A.R., De La Mata, M., Fededa, J.P., Muñoz, M.J. & Nogués, G. (2004) . Multiple links between transcription and splicing. RNA, 10(10), 1489-1498.
http://dx.doi.org/10.1261/rna.7100104
---------- CHICAGO ----------
Kornblihtt, A.R., De La Mata, M., Fededa, J.P., Muñoz, M.J., Nogués, G. "Multiple links between transcription and splicing" . RNA 10, no. 10 (2004) : 1489-1498.
http://dx.doi.org/10.1261/rna.7100104
---------- MLA ----------
Kornblihtt, A.R., De La Mata, M., Fededa, J.P., Muñoz, M.J., Nogués, G. "Multiple links between transcription and splicing" . RNA, vol. 10, no. 10, 2004, pp. 1489-1498.
http://dx.doi.org/10.1261/rna.7100104
---------- VANCOUVER ----------
Kornblihtt, A.R., De La Mata, M., Fededa, J.P., Muñoz, M.J., Nogués, G. Multiple links between transcription and splicing. RNA. 2004;10(10):1489-1498.
http://dx.doi.org/10.1261/rna.7100104