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Abstract:

Implantation is a crucial event in human pregnancy. The participation of cytokines in the implantation process has been widely documented, although the role of many of these molecules is still a matter of controversy. In a previous report from our laboratory, we demonstrated that addition of interferon-γ to the culture medium produces deleterious effects on mouse embryo development. In this study we investigated the effect of this cytokine on outgrowing embryo morphology and on the expression of epidermal growth factor receptors (ErbBs) and heparan sulfate proteoglycan (perlecan) in mouse embryos cultured in vitro. Morphological assessment of inner cell mass and trophoblast development was carried on in-situ fixed and stained outgrowths. Localization of ErbB1, ErbB4 and perlecan on pre- and peri-implantation embryos was investigated by immunocytochemistry. Addition of interferon-γ produced a deleterious effect on both inner cell mass and trophoblast morphology. Immunostaining demonstrated that ErbB1, ErbB4 and perlecan are present on pre-implantation embryos and blastocysts; interferon-γ altered the expression of ErbB4 and Perlecan at the blastocyst stage. We propose that the effects produced by this cytokine could be related to the altered acquisition of adhesion competence and low implantation rates observed in certain reproductive immunological disorders. © 2004 Society for Reproduction and Fertility.

Registro:

Documento: Artículo
Título:Exogenous interferon-γ alters murine inner cell mass and trophoblast development. Effect on the expression of ErbB1, ErbB4 and heparan sulfate proteoglycan (perlecan)
Autor:Fontana, V.; Choren, V.; Vauthay, L.; Calvo, J.C.; Calvo, L.; Cameo, M.
Filiación:Inst. de Oncologia A.H. Roffo, Fac. de Ciencias Exactas/Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Inst. de Biol./Medicina Experimental, Fac. de Ciencias Exactas/Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Department of Biological Chemistry, Fac. de Ciencias Exactas/Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Laboratorio Biologia de Reproduccion, Ecuador 1465 2oB 1425, Buenos Aires, Argentina
Palabras clave:epidermal growth factor receptor; epidermal growth factor receptor 4; gamma interferon; proteoheparan sulfate; adhesion; animal tissue; article; blastocyst; controlled study; embryo; embryo culture; embryo development; female; genital system disease; immunocytochemistry; immunohistochemistry; immunopathology; in vitro study; inner cell mass; morphology; mouse; nonhuman; preimplantation embryo; priority journal; protein expression; protein localization; trophoblast; Animals; Blastocyst; Embryonic Development; Female; Heparan Sulfate Proteoglycans; Immunohistochemistry; Interferon-gamma, Recombinant; Mice; Receptor, Epidermal Growth Factor; Tissue Culture Techniques; Trophoblasts; Murinae
Año:2004
Volumen:128
Número:6
Página de inicio:717
Página de fin:725
DOI: http://dx.doi.org/10.1530/rep.1.00335
Título revista:Reproduction
Título revista abreviado:Reproduction
ISSN:14701626
CODEN:RCUKB
CAS:gamma interferon, 82115-62-6; ERBB4 protein, EC 2.7.1.112; Heparan Sulfate Proteoglycans; Interferon-gamma, Recombinant; perlecan, 143972-95-6; Receptor, Epidermal Growth Factor, EC 2.7.1.112
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14701626_v128_n6_p717_Fontana

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Citas:

---------- APA ----------
Fontana, V., Choren, V., Vauthay, L., Calvo, J.C., Calvo, L. & Cameo, M. (2004) . Exogenous interferon-γ alters murine inner cell mass and trophoblast development. Effect on the expression of ErbB1, ErbB4 and heparan sulfate proteoglycan (perlecan). Reproduction, 128(6), 717-725.
http://dx.doi.org/10.1530/rep.1.00335
---------- CHICAGO ----------
Fontana, V., Choren, V., Vauthay, L., Calvo, J.C., Calvo, L., Cameo, M. "Exogenous interferon-γ alters murine inner cell mass and trophoblast development. Effect on the expression of ErbB1, ErbB4 and heparan sulfate proteoglycan (perlecan)" . Reproduction 128, no. 6 (2004) : 717-725.
http://dx.doi.org/10.1530/rep.1.00335
---------- MLA ----------
Fontana, V., Choren, V., Vauthay, L., Calvo, J.C., Calvo, L., Cameo, M. "Exogenous interferon-γ alters murine inner cell mass and trophoblast development. Effect on the expression of ErbB1, ErbB4 and heparan sulfate proteoglycan (perlecan)" . Reproduction, vol. 128, no. 6, 2004, pp. 717-725.
http://dx.doi.org/10.1530/rep.1.00335
---------- VANCOUVER ----------
Fontana, V., Choren, V., Vauthay, L., Calvo, J.C., Calvo, L., Cameo, M. Exogenous interferon-γ alters murine inner cell mass and trophoblast development. Effect on the expression of ErbB1, ErbB4 and heparan sulfate proteoglycan (perlecan). Reproduction. 2004;128(6):717-725.
http://dx.doi.org/10.1530/rep.1.00335