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Abstract:

The development of modern approaches to the genetic improvement of the tree crops Ilex paraguariensis ('yerba mate') and Ilex dumosa ('yerba señorita') is halted by the scarcity of basic genetic information. In this study, we characterized the implementation of low-cost methodologies such as representational difference analysis (RDA), single-strand conformation polymorphisms (SSCP), and reverse and direct dot-blot filter hybridization assays coupled with thorough bioinformatic characterization of sequence data for both species. Also, we estimated the genome size of each species using flow cytometry. This study contributes to the better understanding of the genetic differences between two cultivated species, by generating new quantitative and qualitative genome-level data. Using the RDA technique, we isolated a group of non-coding repetitive sequences, tentatively considered as Ilex-specific, which were 1.21-to 39.62-fold more abundant in the genome of I. paraguariensis. Another group of repetitive DNA sequences involved retrotransposons, which appeared 1.41-to 35.77-fold more abundantly in the genome of I. dumosa. The genomic DNA of each species showed different performances in filter hybridizations: while I. paraguariensis showed a high intraspecific affinity, I. dumosa exhibited a higher affinity for the genome of the former species (i.e. interspecific). These differences could be attributed to the occurrence of homologous but slightly divergent repetitive DNA sequences, highly amplified in the genome of I. paraguariensis but not in the genome of I. dumosa. Additionally, our hybridization outcomes suggest that the genomes of both species have less than 80% similarity. Moreover, for the first time, we report herein a genome size estimate of 1670 Mbp for I. paraguariensis and that of 1848 Mbp for I. dumosa. © 2014 NIAB.

Registro:

Documento: Artículo
Título:Quantitative and qualitative genomic characterization of cultivated Ilex L. species
Autor:Gottlieb, A.M.; Poggio, L.
Filiación:Laboratorio de Citogenética y Evolución (LaCyE), Departamento de Ecología Genética y Evolución, Universidad de Buenos Aires, 4to. Piso, Pabellón II, Buenos Aires, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Avenida Rivadavia 1917, Ciudad Autónoma de Buenos Aires, C1033AAJ, Argentina
Palabras clave:flow cytometry; genome filter hybridization; representational difference analysis; reverse dot-blot hybridization; Ilex
Año:2014
Volumen:13
Número:3
DOI: http://dx.doi.org/10.1017/S1479262114000756
Título revista:Plant Genetic Resources: Characterisation and Utilisation
Título revista abreviado:Plant Genet. Resour. Charact. Util.
ISSN:14792621
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14792621_v13_n3_p_Gottlieb

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Citas:

---------- APA ----------
Gottlieb, A.M. & Poggio, L. (2014) . Quantitative and qualitative genomic characterization of cultivated Ilex L. species. Plant Genetic Resources: Characterisation and Utilisation, 13(3).
http://dx.doi.org/10.1017/S1479262114000756
---------- CHICAGO ----------
Gottlieb, A.M., Poggio, L. "Quantitative and qualitative genomic characterization of cultivated Ilex L. species" . Plant Genetic Resources: Characterisation and Utilisation 13, no. 3 (2014).
http://dx.doi.org/10.1017/S1479262114000756
---------- MLA ----------
Gottlieb, A.M., Poggio, L. "Quantitative and qualitative genomic characterization of cultivated Ilex L. species" . Plant Genetic Resources: Characterisation and Utilisation, vol. 13, no. 3, 2014.
http://dx.doi.org/10.1017/S1479262114000756
---------- VANCOUVER ----------
Gottlieb, A.M., Poggio, L. Quantitative and qualitative genomic characterization of cultivated Ilex L. species. Plant Genet. Resour. Charact. Util. 2014;13(3).
http://dx.doi.org/10.1017/S1479262114000756