Cystic echinococcosis (CE) is a zoonotic disease caused by the larval stage of the species complex Echinococcus granulosus sensu lato. Within this complex, genotypes G6 and G7 have been frequently associated with human CE worldwide. Previous studies exploring the genetic variability and phylogeography of genotypes G6 and G7 have been based on relatively short mtDNA sequences, and the resolution of these studies has often been low. Moreover, using short sequences, the distinction between G6 and G7 has in some cases remained challenging. The aim here was to sequence complete mitochondrial genomes (mitogenomes) to obtain deeper insight into the genetic diversity, phylogeny and population structure of genotypes G6 and G7. We sequenced complete mitogenomes of 94 samples collected from 15 different countries worldwide. The results demonstrated that (i) genotypes G6 and G7 can be clearly distinguished when mitogenome sequences are used; (ii) G7 is represented by two major haplogroups, G7a and G7b, the latter being specific to islands of Corsica and Sardinia; (iii) intensive animal trade, but also geographical isolation, have likely had the largest impact on shaping the genetic structure and distribution of genotypes G6 and G7. In addition, we found phylogenetically highly divergent haplotype from Mongolia (Gmon), which had a higher affinity to G6. © 2018 Elsevier B.V.
Documento: | Artículo |
Título: | The benefits of analysing complete mitochondrial genomes: Deep insights into the phylogeny and population structure of Echinococcus granulosus sensu lato genotypes G6 and G7 |
Autor: | Laurimäe, T.; Kinkar, L.; Romig, T.; Omer, R.A.; Casulli, A.; Umhang, G.; Gasser, R.B.; Jabbar, A.; Sharbatkhori, M.; Mirhendi, H.; Ponce-Gordo, F.; Lazzarini, L.E.; Soriano, S.V.; Varcasia, A.; Rostami Nejad, M.; Andresiuk, V.; Maravilla, P.; González, L.M.; Dybicz, M.; Gawor, J.; Šarkūnas, M.; Šnábel, V.; Kuzmina, T.; Saarma, U. |
Filiación: | Department of Zoology, Institute of Ecology and Earth Sciences, University of Tartu, Vanemuise 46, Tartu, 51003, Estonia Institute of Zoology, Parasitology Unit, University of Hohenheim, Stuttgart, 70599, Germany National University Research Institute, National University Sudan, Khartoum, Sudan World Health Organization Collaborating Centre for the Epidemiology, Detection and Control of Cystic and Alveolar Echinococcosis (in humans and animals), Istituto Superiore di Sanità, Viale Regina Elena 299, Rome, 00161, Italy European Union Reference Laboratory for Parasites (EURLP), Istituto Superiore di Sanità, Viale Regina Elena 299, Rome, 00161, Italy Anses, Wildlife Surveillance and Eco-epidemiology Unit, National Reference Laboratory for Echinococcus spp., Nancy Laboratory for Rabies and Wildlife, Malzéville, 54220, France Department of Veterinary Biosciences, Melbourne Veterinary School, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria 3010, Australia Laboratory Science Research Center, Golestan University of Medical Sciences, Gorgan, Iran Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran Department of Parasitology, Faculty of Pharmacy, Complutense University, Plaza Ramón y Cajal s/n, Madrid, 28040, Spain Department of Microbiology and Parasitology, Faculty of Medical Sciences, Comahue National University, 1400, Neuquén, Buenos Aires, 8300, Argentina Laboratorio di Parassitologia e Malattie Parassitarie, Ospedale Didattico Veterinario Dipartimento di Medicina Veterinaria, Università degli Studi di Sassari, Via Vienna 2, Sassari, 07100, Italy Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran Laboratorio de Zoonosis Parasitarias, FCEyN, UNMdP, Funes 3350, Mar del Plata, Buenos Aires, 7600, Argentina Hospital General “Dr. Manuel Gea Gonzalez”, Departamento de Ecologia de Agentes PatogenosDF 14080, Mexico Parasitology Department, Centro Nacional de Microbiologia, Instituto de Salud Carlos III, Majadahonda, Madrid, 28220, Spain Department of General Biology and Parasitology, Medical University of Warsaw, 5 Chałubińskiego Str., Warsaw, 02-004, Poland W. Stefański Institute of Parasitology, Polish Academy of Science, Twarda51/55, Warsaw, 00-818, Poland Department of Veterinary Pathobiology, Veterinary Academy, Lithuanian University of Health Sciences, Tilžes Street 18, Kaunas, 47181, Lithuania Institute of Parasitology, Slovak Academy of Sciences, Košice, Hlinkova 3, Košice, 040 01, Slovakia I.I. Schmalhausen Institute of Zoology, National Academy of Sciences of Ukraine, Kyiv, 01030, Ukraine |
Palabras clave: | Cystic echinococcosis; Genotype G6; Genotype G7; Hydatid disease; Mitochondrial genome; Zoonosis; cyclooxygenase 1; Article; binding affinity; Corsica; Echinococcus granulosus; Echinococcus granulosus sensu lato; gene sequence; gene structure; genetic variability; genetic variation; genotype; geographic mapping; geography; haplotype; human; life cycle; mitochondrial genome; Mongolia; nonhuman; phylogeny; population differentiation; population structure; priority journal; Sardinia; sequence analysis |
Año: | 2018 |
Volumen: | 64 |
Página de inicio: | 85 |
Página de fin: | 94 |
DOI: | http://dx.doi.org/10.1016/j.meegid.2018.06.016 |
Título revista: | Infection, Genetics and Evolution |
Título revista abreviado: | Infec. Genet. Evol. |
ISSN: | 15671348 |
CODEN: | IGENC |
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15671348_v64_n_p85_Laurimae |