Artículo

Guimarães, T.D.R.; Quiroz, C.G.; Rigotto, C.; De Oliveira, S.Q.; De Almeida, M.T.R.; Bianco, É.M.; Moritz, M.I.G.; Carraro, J.L.; Palermo, J.A.; Cabrera, G.; Schenkel, E.P.; Reginatto, F.H.; Simões, C.M.O. "Anti HSV-1 activity of halistanol sulfate and halistanol sulfate C isolated from Brazilian marine sponge Petromica citrina (Demospongiae)" (2013) Marine Drugs. 11(11):4176-4192
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Abstract:

The n-butanol fraction (BF) obtained from the crude extract of the marine sponge Petromica citrina, the halistanol-enriched fraction (TSH fraction), and the isolated compounds halistanol sulfate (1) and halistanol sulfate C (2), were evaluated for their inhibitory effects on the replication of the Herpes Simplex Virus type 1 (HSV-1, KOS strain) by the viral plaque number reduction assay. The TSH fraction was the most effective against HSV-1 replication (SI = 15.33), whereas compounds 1 (SI = 2.46) and 2 (SI = 1.95) were less active. The most active fraction and these compounds were also assayed to determine the viral multiplication step(s) upon which they act as well as their potential synergistic effects. The anti-HSV-1 activity detected was mediated by the inhibition of virus attachment and by the penetration into Vero cells, the virucidal effect on virus particles, and by the impairment in levels of ICP27 and gD proteins of HSV-1. In summary, these results suggest that the anti-HSV-1 activity of TSH fraction detected is possibly related to the synergic effects of compounds 1 and 2. © 2013 by the authors; licensee MDPI.

Registro:

Documento: Artículo
Título:Anti HSV-1 activity of halistanol sulfate and halistanol sulfate C isolated from Brazilian marine sponge Petromica citrina (Demospongiae)
Autor:Guimarães, T.D.R.; Quiroz, C.G.; Rigotto, C.; De Oliveira, S.Q.; De Almeida, M.T.R.; Bianco, É.M.; Moritz, M.I.G.; Carraro, J.L.; Palermo, J.A.; Cabrera, G.; Schenkel, E.P.; Reginatto, F.H.; Simões, C.M.O.
Filiación:Laboratory of Natural Products, Department of Pharmaceutical Sciences, Universidade Federal de Santa Catarina, Florianópolis 88040-900, SC, Brazil
Laboratory of Applied Virology, Department of Microbiology, Immunology and Parasitology, Universidade Federal de Santa Catarina, Florianópolis 88040-900, SC, Brazil
Laboratory of Porifera, National Museum, Universidade Federal do Rio de Janeiro, Rio de Janeiro 20940-040, RJ, Brazil
UMYMFOR, Department of Organic Chemistry, University of Buenos Aires, Buenos Aires C1428EGA, Argentina
Palabras clave:Antiviral activity; HSV-1; Marine sponge; Petromica citrina; Sulfate sterols; acetic acid ethyl ester; aciclovir; alcohol; antivirus agent; butanol; gD protein; halistanol; halistanol sulfate; halistanol sulfate C; ICP27 protein; unclassified drug; virus protein; animal tissue; antiviral activity; article; controlled study; drug cytotoxicity; drug isolation; drug potentiation; drug structure; Herpes simplex virus 1; nonhuman; Petromica citrina; sponge (Porifera); Vero cell; virus attachment; virus entry; virus particle; virus replication; virus strain; 1-Butanol; Animals; Antiviral Agents; Brazil; Cell Line; Cercopithecus aethiops; Herpesvirus 1, Human; Porifera; Sterols; Vero Cells; Viral Plaque Assay
Año:2013
Volumen:11
Número:11
Página de inicio:4176
Página de fin:4192
DOI: http://dx.doi.org/10.3390/md11114176
Título revista:Marine Drugs
Título revista abreviado:Mar. Drugs
ISSN:16603397
CODEN:MDARE
CAS:acetic acid ethyl ester, 141-78-6; aciclovir, 59277-89-3; alcohol, 64-17-5; butanol, 35296-72-1, 71-36-3
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_16603397_v11_n11_p4176_Guimaraes

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Citas:

---------- APA ----------
Guimarães, T.D.R., Quiroz, C.G., Rigotto, C., De Oliveira, S.Q., De Almeida, M.T.R., Bianco, É.M., Moritz, M.I.G.,..., Simões, C.M.O. (2013) . Anti HSV-1 activity of halistanol sulfate and halistanol sulfate C isolated from Brazilian marine sponge Petromica citrina (Demospongiae). Marine Drugs, 11(11), 4176-4192.
http://dx.doi.org/10.3390/md11114176
---------- CHICAGO ----------
Guimarães, T.D.R., Quiroz, C.G., Rigotto, C., De Oliveira, S.Q., De Almeida, M.T.R., Bianco, É.M., et al. "Anti HSV-1 activity of halistanol sulfate and halistanol sulfate C isolated from Brazilian marine sponge Petromica citrina (Demospongiae)" . Marine Drugs 11, no. 11 (2013) : 4176-4192.
http://dx.doi.org/10.3390/md11114176
---------- MLA ----------
Guimarães, T.D.R., Quiroz, C.G., Rigotto, C., De Oliveira, S.Q., De Almeida, M.T.R., Bianco, É.M., et al. "Anti HSV-1 activity of halistanol sulfate and halistanol sulfate C isolated from Brazilian marine sponge Petromica citrina (Demospongiae)" . Marine Drugs, vol. 11, no. 11, 2013, pp. 4176-4192.
http://dx.doi.org/10.3390/md11114176
---------- VANCOUVER ----------
Guimarães, T.D.R., Quiroz, C.G., Rigotto, C., De Oliveira, S.Q., De Almeida, M.T.R., Bianco, É.M., et al. Anti HSV-1 activity of halistanol sulfate and halistanol sulfate C isolated from Brazilian marine sponge Petromica citrina (Demospongiae). Mar. Drugs. 2013;11(11):4176-4192.
http://dx.doi.org/10.3390/md11114176