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Abstract:

Water status and metabolite content are considered as two key features in plant cell physiological phenotype. In order to profiling in situ living plant cell status, turgor pressure of cells located at different locations of tissues was probed with a cell pressure probe and then cell sap was sampled and its metabolite profile was generated with using nanoESI and MALDI mass spectrometry. No purification or separation was included in workflow and picoliter cell sap samples were injected directly into a nanoESI-Orbitrap mass spectrometer and/or deposited on selected matrices from organic compounds and nanoparticles for MALDI-TOF mass spectrometry analysis. Both shotgun mass spectrometry techniques could be used for detecting and quantifying metabolites in single-cell samples. Different metabolites from neutral carbohydrates to amino acids and secondary metabolites could be detected. Quantity of two major metabolites, sucrose and kestose, was also measured in several cells and sucrose concentration was co-plotted with turgor data.

Registro:

Documento: Artículo
Título:Integrative analysis of physiological phenotype of plant cells byturgor measurement and metabolomics
Autor:Gholipour, Y.; Erra-Balsells, R.; Nonami, H.
Filiación:Department of Biomechanical Systems, Faculty of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama 780-8566, Japan
Organic Chemistry department, CIHIDECAR University of Buenos Aires, 1428-Buenos Aires, Argentina
Palabras clave:MALDI; Metabolite profiling; NanoESI; Tulip; Cell pressure; Cell sap; Integrative analysis; Kestose; Key feature; MALDI; MALDI-mass spectrometry; MALDI-TOF mass spectrometry; Metabolite content; Metabolite profiles; Metabolite profiling; Metabolomics; Nano-ESI; Plant cells; Secondary metabolites; Spectrometry technique; Sucrose concentration; Tulip; Turgor pressure; Water status; Amino acids; Biomolecules; Carbohydrates; Mass spectrometry; Physiology; Plant cell culture; Plants (botany); Sugar (sucrose); Tissue; Metabolites
Año:2012
Volumen:20
Número:4
Página de inicio:294
Página de fin:300
Título revista:Engineering Letters
Título revista abreviado:Eng. Lett.
ISSN:1816093X
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1816093X_v20_n4_p294_Gholipour

Referencias:

  • Kehr, J., Single cell technology (2003) Curr. Opin. Plant Biol, 6, pp. 617-621
  • Angeles, G., Berrio-Sierra, J., Joseleau, J.P., Lorimier, P., Lefebvre, A., Ruel, K., Preparative laser capture micro-dissection and single pot cell wall material preparation: A novel method for tissue specific analysis (2006) Planta, 224, pp. 228-232
  • Holscher, D., Schneider, B., Laser micro-dissection and cryogenic nuclear magnetic resonance spectroscopy: An alliance for cell type-specific metabolite profiling (2007) Planta, 225, pp. 763-770
  • Li, S.H., Schneider, B., Gershenzon, J., Microchemical analysis of laser-microdissected stone cells of Norway spruce by cryogenic nuclear magnetic resonance spectroscopy (2007) Planta, 225, pp. 771-779
  • Shrestha, B., Vertes, A., In situ metabolic profiling of single cells by laser ablation electrospray ionization mass spectrometry (2009) Anal. Chem, 81, pp. 8265-8271
  • Yu, Z., Chen, L.C., Suzuki, H., Ariyada, O., Erra-Balsells, R., Nonami, H., Hiraoka, K., Direct profiling of phytochemicals in tulip tissues and in vivo monitoring of the change of carbohydrate content in tulip bulbs by probe electrospray ionization mass spectrometry (2009) J. Am. Soc. Mass Spectrom, 20, pp. 2304-2311
  • Hüsken, D., Steudle, E., Zimmermann, U., Pressure probe technique for measuring water relations of cells in higher plants (1978) Plant Physiol, 61, pp. 158-163
  • Gholipour, Y., Nonami, H., Erra-Balsells, R., Application of pressure probe and UV-MALDI-TOF MS for direct analysis of plant underivatized carbohydrates in subpicoliter single-cell cytoplasm extract (2008) J. Am. Soc. Mass Spectrom, 19, pp. 1841-1848
  • Malone, M., Leigh, R.A., Tomos, A.D., Extraction and analysis of sap from individual wheat leaf cells: The effect of sampling speed on the osmotic pressure of extracted sap (1987) Plant Cell Environ, 12, pp. 919-926
  • Malone, M., Leigh, R.A., Tomos, A.D., Concentration of Vacuolar Inorganic Ions in Individual Cells Of Intact Wheat Leaf Epidermis (1991) J. Exp. Bot, 42, pp. 305-309
  • Koroleva, O.A., Farrar, J.F., Tomos, A.D., Pollock, C.J., Patterns of solute in individual mesophyll, bundle sheath and epidermal cells of barley leaves induced to accumulate carbohydrate (1997) New Phytol, 136, pp. 97-104
  • Koroleva, O.A., Farrar, J.F., Tomos, A.D., Pollock, C.J., Carbohydrates in individual cells of epidermis, mesophyll, and bundle sheath in barley leaves with changed export or photosynthetic (1998) Plant Physiol, 118, pp. 1525-1532
  • Korolev, A.V., Tomos, A.D., Bowtell, R., Farrar, J.F., Spatial and temporal distribution of solutes in the developing carrot taproot measured at single-cell resolution (2000) J. Exp. Bot, 51, pp. 567-577
  • Tomos, A.D., Sharrock, R.A., Cell sampling and analysis (SiCSA): Metabolites measured at single cell resolution (2001) J. Exp. Bot, 52, pp. 623-630
  • Markey, S.P., Quantitative mass spectrometry (1981) Biol. Mass Spectrom, 8, pp. 426-430
  • Harvey, D.J., Quantitative aspects of the matrix-assisted laser desorption mass spectrometry of complex oligosaccharides (1993) Mass Rapid Commun. in Spectrom, 7, pp. 614-619
  • Bantscheff, M., Schirle, M., Sweetman, G., Rick, J., Bernhard, J., Kuster, B., Quantitative mass spectrometry in proteomics: A critical review (2007) Anal. Bioanal. Chem, 389, pp. 1618-2650
  • Gholipour, Y., Nonami, H., Erra-Balsells, R., Diamond, titanium dioxide, titanium silicon oxide and barium strontium titanium oxide nanoparticles as matrices for direct matrix-assisted laser desorption/ionization mass spectrometry analysis of carbohydrates in plant tissue (2010) Anal. Chem, 82, pp. 5518-5526
  • Kramer, P.J., Boyer, J.S., (1995) Water Relations of Plants and Soils, , Academic Press, San Diego
  • Boyer, J.S., (1995) Measuring the Water Status of Plants and Soils, , Academic Press, San Diego
  • Nonami, H., (2001) Plant Water Relations, , (Japanese in text), Yokendo, Tokyo
  • Gholipour, Y., Erra-Balsells, R., Nonami, H., In situ pressure probe sampling and UVMALDI MS for profiling metabolites in living single cells (2012) Mass Spectrom, p. 1. , DOI: 10.5702/massspectrometry. A0003
  • Erra-Balsells, R., Gholipour, Y., Nonami, H., Situ Pressure Probe Sampling of Single Cell Solution From Living Plants For Metabolite Analyses With UV-MALDI MS, Lecture Notes In Systems Biology and Bioengineering: Proceedings of the World Congress On Engineering 2012, pp. 572-577. , WCE 2012, 4-6 July, 2012, London, U.K
  • Moe, R., Wickström, A., A, The effect of storage temperature on shoot growth, flowering, and carbohydrate metabolism in tulip bulbs (1973) Physiol Plant, 28, pp. 81-87
  • Lambrechts, H., Rook, F., Kolloffel, C., C, Carbohydrate status of tulip bulbs during cold-induced flower stalk elongation and flowering (1994) Plant Physiol, 104, pp. 515-520
  • Steudle, E., Zimmermann, U., Effect of turgor pressure and cell size on the wall elasticity of plant cells (1977) Plant Physiol, 59, p. 285
  • Green, P.B., Erickson, R.O., Buggy, J., Metabolic and physical control of cell elongation rate: In vivo studies in nitella (1971) Plant Physiol, 47, p. 423
  • Nonami, H., Boyer, J.S., Wall extensibility and cell hydraulic conductivity decrease in enlarging stem tissues at low water potentials (1990) Plant Physiol, 93, pp. 1610-1619
  • Nonami, H., Boyer, J.S., Direct demonstration of a growth-induced water potential gradient (1993) Plant Physiol, 102, pp. 13-19
  • Malone, M., Tomos, A.D., A simple pressure-probe method for the determination of volume in higher-plant cells (1990) Planta, 182, pp. 199-203
  • Shackel, K.A., Direct measurement of turgor and osmotic potential in individual epidermal cells: Independent confirmation of leaf water potential as determined by in situ psychrometry (1987) Plant Physiol, 83, pp. 719-722
  • Nonami, H., Schulze, E.D., Cell water potential, osmotic potential, and turgor in the epidermis and mesophyll of transpiring leaves: Combined measurements with the cell pressure probe and nanoliter osmometer (1989) Planta, 171, pp. 35-46
  • Gholipour, Y., Nonami, H., Erra-Balsells, R., In situ analysis of plant tissue underivatized carbohydrates and on-probe enzymatic degraded starch by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry by using carbon nanotubes as matrix (2008) Analytical Biochemistry, 383, pp. 159-167
  • Stahl, B., Linos, A., Karas, M., Hillenkamp, F., Steup, M., Analysis of fructans from higher plants by matrix-assisted laser desorption/ionization mass spectrometry (1997) Anal. Biochem, 246, pp. 195-204
  • Harvey, D.J., Analysis of carbohydrates and glycoconjugates by matrix-assisted laser desorption/ionization mass spectrometry: An update covering the period 2001-2002 (2008) Mass Spectrom. Rev, 27, pp. 125-201

Citas:

---------- APA ----------
Gholipour, Y., Erra-Balsells, R. & Nonami, H. (2012) . Integrative analysis of physiological phenotype of plant cells byturgor measurement and metabolomics. Engineering Letters, 20(4), 294-300.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1816093X_v20_n4_p294_Gholipour [ ]
---------- CHICAGO ----------
Gholipour, Y., Erra-Balsells, R., Nonami, H. "Integrative analysis of physiological phenotype of plant cells byturgor measurement and metabolomics" . Engineering Letters 20, no. 4 (2012) : 294-300.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1816093X_v20_n4_p294_Gholipour [ ]
---------- MLA ----------
Gholipour, Y., Erra-Balsells, R., Nonami, H. "Integrative analysis of physiological phenotype of plant cells byturgor measurement and metabolomics" . Engineering Letters, vol. 20, no. 4, 2012, pp. 294-300.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1816093X_v20_n4_p294_Gholipour [ ]
---------- VANCOUVER ----------
Gholipour, Y., Erra-Balsells, R., Nonami, H. Integrative analysis of physiological phenotype of plant cells byturgor measurement and metabolomics. Eng. Lett. 2012;20(4):294-300.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1816093X_v20_n4_p294_Gholipour [ ]