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Maymó, J.L.; Riedel, R.; Pérez-Pérez, A.; Magatti, M.; Maskin, B.; Dueñas, J.L.; Parolini, O.; Sánchez-Margalet, V.; Varone, C.L. "Proliferation and survival of human amniotic epithelial cells during their hepatic differentiation" (2018) PLoS ONE. 13(1)
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Abstract:

Stem cells derived from placental tissues are an attractive source of cells for regenerative medicine. Amniotic epithelial cells isolated from human amnion (hAECs) have desirable and competitive characteristics that make them stand out between other stem cells. They have the ability to differentiate toward all three germ layers, they are not tumorigenic and they have immunosuppressive properties. Although liver transplantation is the best way to treat acute and chronic hepatic failure patients, there are several obstacles. Recently, stem cells have been spotlighted as alternative source of hepatocytes because of their potential for hepatogenic differentiation. In this work, we aimed to study the proliferation and survival of the hAECs during their hepatic differentiation. We have also analyzed the changes in pluripotency and hepatic markers. We differentiated amniotic cells applying a specific hepatic differentiation (HD) protocol. We determined by qRT-PCR that hAECs express significant levels of SOX-2, OCT-4 and NANOG during at least 15 days in culture and these pluripotent markers diminish during HD. SSEA-4 expression was reduced during HD, measured by immunofluorescence. Morphological characteristics became more similar to hepatic ones in differentiated cells and representative hepatic markers significantly augmented their expression, measured by qRT-PCR and Western blot. Cells achieved a differentiation efficiency of 75%. We observed that HD induced proliferation and promoted survival of hAECs, during 30 days in culture, evaluated by 3H-thymidine incorporation and MTT assay. HD also promoted changes in hAECs cell cycle. Cyclin D1 expression increased, while p21 and p53 levels were reduced. Immunofluorescence analysis showed that Ki-67 expression was upregulated during HD. Finally, ERK 1/2 phosphorylation, which is intimately linked to proliferation and cell survival, augmented during all HD process and the inhibition of this signaling pathway affected not only proliferation but also differentiation. Our results suggest that HD promotes proliferation and survival of hAECs, providing important evidence about the mechanisms governing their hepatic differentiation. We bring new knowledge concerning some of the optimal transplantation conditions for these hepatic like cells. Copyright: © 2018 Maymó et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Registro:

Documento: Artículo
Título:Proliferation and survival of human amniotic epithelial cells during their hepatic differentiation
Autor:Maymó, J.L.; Riedel, R.; Pérez-Pérez, A.; Magatti, M.; Maskin, B.; Dueñas, J.L.; Parolini, O.; Sánchez-Margalet, V.; Varone, C.L.
Filiación:Universidad de Buenos Aires, CONICET, Instituto de Química Biológica, Facultad de Ciencias Exactas y Naturales (IQUIBICEN), Ciudad Universitaria Pabellón 2, 4 piso, Buenos Aires, 1428, Argentina
Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Química Biológica, Ciudad Universitaria Pabellón 2, 4 piso, Buenos Aires, 1428, Argentina
Departamento de Bioquímica Médica y Biología Molecular, Hospital Universitario Virgen Macarena, Facultad de Medicina, Universidad de Sevilla, Avenida Sánchez Pizjuán 4, Sevilla, 41009, Spain
Centro di Ricerca E. Menni-Fondazione Poliambulanza, Istituto Ospedaliero, Brescia, Italy
Hospital Nacional Profesor Alejandro Posadas, Buenos Aires, Argentina
Servicio de Ginecología y Obstetricia, Hospital Universitario Virgen Macarena, Sevilla, Spain
Palabras clave:cyclin D1; Ki 67 antigen; mitogen activated protein kinase 1; mitogen activated protein kinase 3; octamer transcription factor 4; protein p21; protein p53; stage specific embryo antigen 4; transcription factor NANOG; transcription factor Sox2; biological marker; amnion cell; amniotic epithelial cell; Article; cell culture; cell cycle; cell differentiation; cell proliferation; cell structure; cell survival; controlled study; enzyme phosphorylation; human; human cell; immunofluorescence; liver cell; pluripotent stem cell; protein expression; reverse transcription polymerase chain reaction; upregulation; Western blotting; amnion; cytology; epithelium cell; female; liver; MAPK signaling; metabolism; phosphorylation; pregnancy; real time polymerase chain reaction; Amnion; Biomarkers; Cell Proliferation; Cell Survival; Cells, Cultured; Epithelial Cells; Female; Humans; Liver; MAP Kinase Signaling System; Phosphorylation; Pregnancy; Real-Time Polymerase Chain Reaction
Año:2018
Volumen:13
Número:1
DOI: http://dx.doi.org/10.1371/journal.pone.0191489
Título revista:PLoS ONE
Título revista abreviado:PLoS ONE
ISSN:19326203
CODEN:POLNC
CAS:mitogen activated protein kinase 1, 137632-08-7; mitogen activated protein kinase 3, 137632-07-6; protein p21, 85306-28-1; Biomarkers
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v13_n1_p_Maymo

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Citas:

---------- APA ----------
Maymó, J.L., Riedel, R., Pérez-Pérez, A., Magatti, M., Maskin, B., Dueñas, J.L., Parolini, O.,..., Varone, C.L. (2018) . Proliferation and survival of human amniotic epithelial cells during their hepatic differentiation. PLoS ONE, 13(1).
http://dx.doi.org/10.1371/journal.pone.0191489
---------- CHICAGO ----------
Maymó, J.L., Riedel, R., Pérez-Pérez, A., Magatti, M., Maskin, B., Dueñas, J.L., et al. "Proliferation and survival of human amniotic epithelial cells during their hepatic differentiation" . PLoS ONE 13, no. 1 (2018).
http://dx.doi.org/10.1371/journal.pone.0191489
---------- MLA ----------
Maymó, J.L., Riedel, R., Pérez-Pérez, A., Magatti, M., Maskin, B., Dueñas, J.L., et al. "Proliferation and survival of human amniotic epithelial cells during their hepatic differentiation" . PLoS ONE, vol. 13, no. 1, 2018.
http://dx.doi.org/10.1371/journal.pone.0191489
---------- VANCOUVER ----------
Maymó, J.L., Riedel, R., Pérez-Pérez, A., Magatti, M., Maskin, B., Dueñas, J.L., et al. Proliferation and survival of human amniotic epithelial cells during their hepatic differentiation. PLoS ONE. 2018;13(1).
http://dx.doi.org/10.1371/journal.pone.0191489