Artículo

Traglia, G.M.; Sala, C.D.; Bass, J.I.F.; Soler-Bistué, A.J.C.; Zorreguieta, A.; Ramírez, M.S.; Tolmasky, M.E. "Internalization of locked nucleic acids/DNA hybrid oligomers into escherichia coli" (2012) BioResearch Open Access. 1(5):260-263
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Abstract:

Delivery inside the cells is essential for practical application of antisense technologies. The hybrid locked nucleic acid (LNA)/DNA CAAGTACTGTTCCACCA (LNA residues are underlined) was labeled by conjugation to Alexa Fluor 488 (fLNA/DNA) and tested to determine its ability to penetrate Escherichia coli cells and reach the cytoplasm. Flow cytometry analysis showed that the fLNA/DNA was associated with 14% of cells from a sta- tionary phase culture, while association with a labeled isosequential oligodeoxynucleotide was negligible. Laser scanning confocal microscopy confirmed that the fLNA/DNA was located inside the cytoplasm.© Mary Ann Liebert, Inc.

Registro:

Documento: Artículo
Título:Internalization of locked nucleic acids/DNA hybrid oligomers into escherichia coli
Autor:Traglia, G.M.; Sala, C.D.; Bass, J.I.F.; Soler-Bistué, A.J.C.; Zorreguieta, A.; Ramírez, M.S.; Tolmasky, M.E.
Filiación:Institute of Microbiology and Medical Parasitology, National Scientific and Technical Research Council (CONICET), University of Buenos Aires, Buenos Aires, Argentina
Leloir Foundation Institute, Biochemical Research Institute of Buenos Aires (IBBA), CONICET; Department of Biological Chemistry, Faculty of Natural Sciences (FCEyN); University of Buenos Aires, Buenos Aires, Argentina
Department of Immunology, Hematological Research Institute, Maissa Foundation Oncological Studies Institute; National Academy of Medicine, Buenos Aires, Argentina
Center for Applied Biotechnology Studies, Department of Biological Science, California State University, Fullerton, CA, United States
Program in Systems Biology, University of Massachusetts Medical School, Worcester, MA, United States
Bacterial Genome Plasticity Unit, Department of Genomes and Genetics, Institut Pasteur, Paris, France
Palabras clave:Drug discovery; Drug resistance; Gene therapy; Microbiology; Nucleic acids; alexa fluor 488; locked nucleic acid; oligomer; unclassified drug; antisense therapy; article; bacterial cell; cell culture; confocal microscopy; conjugation; controlled study; cytoplasm; drug penetration; Escherichia coli; flow cytometry; gene delivery system; nonhuman; priority journal
Año:2012
Volumen:1
Número:5
Página de inicio:260
Página de fin:263
DOI: http://dx.doi.org/10.1089/biores.2012.0257
Título revista:BioResearch Open Access
Título revista abreviado:BioResearch Open Access
ISSN:21647860
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_21647860_v1_n5_p260_Traglia

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Citas:

---------- APA ----------
Traglia, G.M., Sala, C.D., Bass, J.I.F., Soler-Bistué, A.J.C., Zorreguieta, A., Ramírez, M.S. & Tolmasky, M.E. (2012) . Internalization of locked nucleic acids/DNA hybrid oligomers into escherichia coli. BioResearch Open Access, 1(5), 260-263.
http://dx.doi.org/10.1089/biores.2012.0257
---------- CHICAGO ----------
Traglia, G.M., Sala, C.D., Bass, J.I.F., Soler-Bistué, A.J.C., Zorreguieta, A., Ramírez, M.S., et al. "Internalization of locked nucleic acids/DNA hybrid oligomers into escherichia coli" . BioResearch Open Access 1, no. 5 (2012) : 260-263.
http://dx.doi.org/10.1089/biores.2012.0257
---------- MLA ----------
Traglia, G.M., Sala, C.D., Bass, J.I.F., Soler-Bistué, A.J.C., Zorreguieta, A., Ramírez, M.S., et al. "Internalization of locked nucleic acids/DNA hybrid oligomers into escherichia coli" . BioResearch Open Access, vol. 1, no. 5, 2012, pp. 260-263.
http://dx.doi.org/10.1089/biores.2012.0257
---------- VANCOUVER ----------
Traglia, G.M., Sala, C.D., Bass, J.I.F., Soler-Bistué, A.J.C., Zorreguieta, A., Ramírez, M.S., et al. Internalization of locked nucleic acids/DNA hybrid oligomers into escherichia coli. BioResearch Open Access. 2012;1(5):260-263.
http://dx.doi.org/10.1089/biores.2012.0257