Artículo

Peña, D.; Rovetta, A.I.; Hernández Del Pino, R.E.; Amiano, N.O.; Pasquinelli, V.; Pellegrini, J.M.; Tateosian, N.L.; Rolandelli, A.; Gutierrez, M.; Musella, R.M.; Palmero, D.J.; Gherardi, M.M.; Iovanna, J.; Chuluyan, H.E.; García, V.E. "A Mycobacterium tuberculosis Dormancy Antigen Differentiates Latently Infected Bacillus Calmette-Guérin-vaccinated Individuals" (2015) EBioMedicine. 2(8):884-890
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Abstract:

IFN-γ release assays (IGRAs) are better indicators of Mycobacterium tuberculosis infection than the tuberculin skin test (TST) in Bacillus Calmette-Guérin (BCG)-vaccinated populations. However, IGRAs do not discriminate active and latent infections (LTBI) and no gold standard for LTBI diagnosis is available. Thus, since improved tests to diagnose M. tuberculosis infection are required, we assessed the efficacy of several M. tuberculosis latency antigens. BCG-vaccinated healthy donors (HD) and tuberculosis (TB) patients were recruited. QuantiFERON-TB Gold In-Tube, TST and clinical data were used to differentiate LTBI. IFN-γ production against CFP-10, ESAT-6, Rv2624c, Rv2626c and Rv2628 antigens was tested in peripheral blood mononuclear cells. LTBI subjects secreted significantly higher IFN-γ levels against Rv2626c than HD. Additionally, Rv2626c peptide pools to which only LTBI responded were identified, and their cumulative IFN-γ response improved LTBI discrimination. Interestingly, whole blood stimulation with Rv2626c allowed the discrimination between active and latent infections, since TB patients did not secrete IFN-γ against Rv2626c, in contrast to CFP-10. +. ESAT-6 stimulation that induced IFN-γ response from both LTBI and TB patients. ROC analysis confirmed that Rv2626c discriminated LTBI from HD and TB patients. Therefore, since only LTBI recognizes specific epitopes from Rv2626c, this antigen could improve LTBI diagnosis, even in BCG-vaccinated people. © 2015 The Authors.

Registro:

Documento: Artículo
Título:A Mycobacterium tuberculosis Dormancy Antigen Differentiates Latently Infected Bacillus Calmette-Guérin-vaccinated Individuals
Autor:Peña, D.; Rovetta, A.I.; Hernández Del Pino, R.E.; Amiano, N.O.; Pasquinelli, V.; Pellegrini, J.M.; Tateosian, N.L.; Rolandelli, A.; Gutierrez, M.; Musella, R.M.; Palmero, D.J.; Gherardi, M.M.; Iovanna, J.; Chuluyan, H.E.; García, V.E.
Filiación:Instituto de Química Biológica, Facultad de Ciencias Exactas y Naturales (IQUIBICEN), UBA (Universidad de Buenos Aires)-CONICET, Intendente Guiraldes 2160, Pabellon II, 4piso Ciudad Univ., Buenos Aires, C1428EGA, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, UBA, Ciudad Universitaria, Intendente Guiraldes 2160, Pabellon II, 4piso, Buenos Aires, C1428EGA, Argentina
Sección Bacteriología de la Tuberculosis, Hospital General de Agudos Dr. E. Tornu, Combatientes de Malvinas 3002, Buenos Aires, 1427, Argentina
División Tisioneumonología Hospital F.J. Muñiz, Uspallata 2272, Buenos Aires, C1282AEN, Argentina
INBIRS, Facultad de Medicina, UBA, Paraguay 2155, Buenos Aires, C1121ABG, Argentina
Centre de Recherche en Cancérologie de Marseille (CRCM), INSERM U1068, CNRS UMR 7258, Aix-Marseille Universite Institut Paoli-Calmettes, Parc Scientifique et Technologique de Luminy, Marseille, France
Centro de Estudios Farmacológicos y Botánicos (CEFYBO), Facultad de Medicina, UBA, Paraguay 2155, Buenos Aires, C1121ABG, Argentina
Palabras clave:Active tuberculosis; Antigens; Diagnosis; IFN-gamma; Latent tuberculosis; epitope; gamma interferon; bacterial antigen; BCG vaccine; gamma interferon; IFNG protein, human; adult; Article; BCG vaccination; controlled study; enzyme linked immunosorbent assay; female; flow cytometry; human; latent virus infection; major clinical study; male; Mycobacterium tuberculosis; nonhuman; peripheral blood mononuclear cell; priority journal; sequence analysis; tuberculin test; tuberculosis; immunology; middle aged; mononuclear cell; Mycobacterium bovis; Adult; Antigens, Bacterial; Female; Humans; Interferon-gamma; Leukocytes, Mononuclear; Male; Middle Aged; Mycobacterium bovis; Mycobacterium tuberculosis; Tuberculosis Vaccines
Año:2015
Volumen:2
Número:8
Página de inicio:884
Página de fin:890
DOI: http://dx.doi.org/10.1016/j.ebiom.2015.05.026
Título revista:EBioMedicine
Título revista abreviado:EBioMedicine
ISSN:23523964
CAS:gamma interferon, 82115-62-6; Antigens, Bacterial; IFNG protein, human; Interferon-gamma; Tuberculosis Vaccines
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_23523964_v2_n8_p884_Pena

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Citas:

---------- APA ----------
Peña, D., Rovetta, A.I., Hernández Del Pino, R.E., Amiano, N.O., Pasquinelli, V., Pellegrini, J.M., Tateosian, N.L.,..., García, V.E. (2015) . A Mycobacterium tuberculosis Dormancy Antigen Differentiates Latently Infected Bacillus Calmette-Guérin-vaccinated Individuals. EBioMedicine, 2(8), 884-890.
http://dx.doi.org/10.1016/j.ebiom.2015.05.026
---------- CHICAGO ----------
Peña, D., Rovetta, A.I., Hernández Del Pino, R.E., Amiano, N.O., Pasquinelli, V., Pellegrini, J.M., et al. "A Mycobacterium tuberculosis Dormancy Antigen Differentiates Latently Infected Bacillus Calmette-Guérin-vaccinated Individuals" . EBioMedicine 2, no. 8 (2015) : 884-890.
http://dx.doi.org/10.1016/j.ebiom.2015.05.026
---------- MLA ----------
Peña, D., Rovetta, A.I., Hernández Del Pino, R.E., Amiano, N.O., Pasquinelli, V., Pellegrini, J.M., et al. "A Mycobacterium tuberculosis Dormancy Antigen Differentiates Latently Infected Bacillus Calmette-Guérin-vaccinated Individuals" . EBioMedicine, vol. 2, no. 8, 2015, pp. 884-890.
http://dx.doi.org/10.1016/j.ebiom.2015.05.026
---------- VANCOUVER ----------
Peña, D., Rovetta, A.I., Hernández Del Pino, R.E., Amiano, N.O., Pasquinelli, V., Pellegrini, J.M., et al. A Mycobacterium tuberculosis Dormancy Antigen Differentiates Latently Infected Bacillus Calmette-Guérin-vaccinated Individuals. EBioMedicine. 2015;2(8):884-890.
http://dx.doi.org/10.1016/j.ebiom.2015.05.026