Cryptic diversity of the subfamily Calaphidinae (Hemiptera: Aphididae) revealed bycomprehensive DNA barcoding
Authors:
- Yerim Lee,
- Wonhoon Lee,
- Mariusz Kanturski,
- Robert G Foottit,
- Shin-Ichi Akimoto,
- Seunghwan Lee
Abstract
Aphids are a species rich group comprising many important pests. However, species identification can be very difficult for aphids due to their morphological ambiguity. DNA barcoding has been widely adopted for rapid and reliable species identification as well as cryptic species detection. In this study, we investigated cryptic diversity in the subfamily Calaphidinae (Hemiptera: Aphididae) based on 899 sequences of cytochrome c oxidase I (COI) for 115 morphospecies (78 species collected in this study and sequences of 73 species downloaded from Genbank). Among these 115 morphospecies, DNA barcoding results of 90 (78.3%) species were identical to results of morphological identification. However, 25 (21.7%) morphospecies showed discrepancies between DNA barcoding and traditional taxonomy. Among these 25 discordances, a total of 15 cryptic species were identified from 12 morphospecies. We also found three morphologically distinct species pairs that sharing DNA barcoding. Based on molecular operational taxonomic unit (MOTU) estimation, we discussed on species delimitation threshold value for these taxa. Our findings confirm that Calaphidinae has high cryptic diversity even though aphids are relatively well-studied.
- Record ID
- USL4177b318b1254cf99c44518c69cf514c
- Author
- Journal series
- PLoS ONE, ISSN 1932-6203
- Issue year
- 2017
- No
- 4
- Pages
- 1-30
- Keywords in English
- Calaphidinae; DNA barcoding; DNA extraction; genetic variation; Hemiptera; molecular biology; nonhuman; nucleotide sequence; species diversity; species identification; unindexed sequence; animal; aphid; classification; DNA barcoding; genetics; phylogeny
- DOI
- DOI:10.1371/journal.pone.0176582 Opening in a new tab
- Handle.net URL
- hdl.handle.net/20.500.12128/295 Opening in a new tab
- URL
- https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0176582 Opening in a new tab
- Language
- eng (en) English
- License
- File
-
- File: 1
- Cryptic diversity of the subfamily Calaphidinae (Hemiptera: Aphididae) revealed bycomprehensive DNA barcoding, File Kanturski_Cryptic_diversity_of_the_subfamily.pdf / 20 MB
- Kanturski_Cryptic_diversity_of_the_subfamily.pdf
- publication date: 23-08-2023
- Cryptic diversity of the subfamily Calaphidinae (Hemiptera: Aphididae) revealed bycomprehensive DNA barcoding, File Kanturski_Cryptic_diversity_of_the_subfamily.pdf / 20 MB
-
- Score (nominal)
- 40
- Score source
- BIBLIOGRAFIA DOROBKU PRACOWNIKÓW UŚ
- Publication indicators
- Citation count
- 32
- PubMed ID
- 28448639 Opening in a new tab
- Uniform Resource Identifier
- https://opus.us.edu.pl/info/article/USL4177b318b1254cf99c44518c69cf514c/
- URN
urn:uni-kat-prod:USL4177b318b1254cf99c44518c69cf514c
* presented citation count is obtained through Internet information analysis, and it is close to the number calculated by the Publish or PerishOpening in a new tab system.