DNA methylation patterns of Brachypodium distachyon chromosomes and their alteration by 5-azacytidine treatment
Authors:
- Natalia Magdalena Borowska-Żuchowska,
- Dominika Idziak-Helmcke,
- Robert Rudolf Hasterok
Abstract
Sequential immunolocalisation of 5-methylcytosine (5-MeC) and fluorescence in situ hybridisation with chromosome-specific BAC clones were performed on Brachypodium distachyon mitotic metaphase chromosomes to determine specific DNA methylation patterns of each chromosome in the complement. In the majority of cells examined, chromosomes Bd4 and Bd5, which bear the loci of 5S and 35S ribosomal DNA, respectively, had characteristic 5-MeC patterns. In contrast, the distribution of 5-MeC along the metacentric chromosome pairs Bd1, Bd2 and Bd3 was more variable. There were numerous differences in distribution of methylated sites between homologous chromosomes as well as between chromosome arms. Some chromosome sites, such as pericentromeric regions, were highly methylated in all chromosomes. Additionally, the influence of a hypomethylating agent, 5-azacytidine, on B. distachyon chromosome methylation patterns was confirmed. It was found that some chromosome pairs underwent demethylation more easily than others, but there was no apparent regularity in demethylation of particular chromosome segments.
- Record ID
- USLa4cf0cd6f5a24ee7a468298aff749af0
- Author
- Journal series
- Chromosome Research, ISSN 0967-3849, e-ISSN 1573-6849
- Issue year
- 2011
- Vol
- 19
- No
- 8
- Pages
- 955-967
- Publication size in sheets
- 0.60
- Keywords in English
- 5-azacytidine; Brachypodium distachyon; Chromosome; DNA methylation; Epigenetics
- DOI
- DOI:10.1007/s10577-011-9243-2 Opening in a new tab
- Handle.net URL
- hdl.handle.net/20.500.12128/9028 Opening in a new tab
- URL
- https://link.springer.com/content/pdf/10.1007/s10577-011-9243-2.pdf Opening in a new tab
- Language
- eng (en) English
- File
-
- File: 1
- DNA methylation patterns of Brachypodium distachyon chromosomes and their alteration by 5-azacytidine treatment, File Borowska_DNA_methylation_patterns_of_Brachypodium_distachyon.pdf / 858 KB
- Borowska_DNA_methylation_patterns_of_Brachypodium_distachyon.pdf
- publication date: 06-02-2024
- DNA methylation patterns of Brachypodium distachyon chromosomes and their alteration by 5-azacytidine treatment, File Borowska_DNA_methylation_patterns_of_Brachypodium_distachyon.pdf / 858 KB
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- Score (nominal)
- 10
- Score source
- BIBLIOGRAFIA DOROBKU PRACOWNIKÓW UŚ
- Publication indicators
- Citation count
- 17
- Uniform Resource Identifier
- https://opus.us.edu.pl/info/article/USLa4cf0cd6f5a24ee7a468298aff749af0/
- URN
urn:uni-kat-prod:USLa4cf0cd6f5a24ee7a468298aff749af0
* presented citation count is obtained through Internet information analysis, and it is close to the number calculated by the Publish or PerishOpening in a new tab system.