A Modified Coupled Spectrophotometric Method to Detect 2-5 Oligoadenylate Synthetase Activity in Prostate Cell Lines

Bhosle, Sushma M., Clark Atlanta University
Hunt, Aisha, Clark Atlanta University
Chaudhary, Jaideep, Clark Atlanta University

2016-01-01

2010-2019

Background: 2-5 oligoadenylate synthetases (OAS) are interferon inducible enzymes that polymerizes ATP to 2-5-linked oligomers of adenylate (2-5As). As part of the innate immune response, these enzymes are activated by viral double stranded RNA or mRNAs with significant double stranded structure. The 2-5As in turn activate RNaseL that degrade single stranded RNAs. Three distinct forms of OAS exist in human cells (OAS1, 2 and 3) with each form having multiple spliced variants. The OAS enzymes and their spliced variants have different enzyme activities. OAS enzymes also play a significant role in regulating multiple cellular processes such as proliferation and apoptosis. Moreover, Single nucleotide polymorphisms that alter OAS activity are also associated with viral infection, diabetes and cancer. Thus detection of OAS enzyme activity with a simple spectrophotometric method in cells will be important in clinical research.

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Biological Procedures Online 18, no. 9 (2016)

Clark Atlanta University

http://dx.doi.org/10.1186/s12575-016-0038-x.

10.1186/s12575-016-0038-x.

http://hdl.handle.net/20.500.12322/cau.ir:2016_bhosle_sushma

http://rightsstatements.org/vocab/InC/1.0/