The development and design of fluorescent sensors for continuous in vivo glucose monitoring

Permanent URL: http://hdl.handle.net/2047/d20004844
Title:
The development and design of fluorescent sensors for continuous in vivo glucose monitoring
Creator:
Balaconis, Mary Katharine (Author)
Contributor:
Clark, Heather (Advisor)
Amiji, Mansoor M. (Committee member)
Makowski, Lee (Committee member)
Publisher:
Boston, Massachusetts : Northeastern University, 2013
Copyright date:
2013
Date Accepted:
December 2013
Date Awarded:
January 2014
Type of resource:
Text
Genre:
Dissertations
Format:
electronic
Digital origin:
born digital
Abstract/Description:
Diabetes is an international health epidemic characterized by hypo and hyperglycemic levels. To maintain glycemic control, diabetics test glucose levels several times a day. However, the current glucose monitoring approaches suffer from invasiveness, intermittent measurements, accuracy, and lifetime. This dissertation details the design of novel fluorescent glucose-sensitive sensors for monitoring glucose levels non-invasively and continuously after initial implantation. Sensing components were selected for appropriate response at physiological concentrations and were fully characterized for dynamic range, sensitivity, and lifetime in vitro. In preclinical testing, glucose-sensitive sensors tracked changes in glucose levels in mice, but sensor monitoring was limited to one hour. Sensor design was further improved after these studies to prolong in vivo lifetime, increase response at hypoglycemic levels, and enhance sensor biocompatibility. These efforts resulted in in vivo lifetimes greater than one hour, incorporation of more advance sensing moieties, and a biodegradable sensor platform. Future work with these sensors will involve Clark Error Grid Analysis and biodistribution studies to address clinical application requirements.
Subjects and keywords:
diabetes
glucose monitoring
fluorescent sensors
Biomedical devices and instrumentation
Biomedical Engineering and Bioengineering
Mechanical Engineering
Nanoscience and Nanotechnology
DOI:
https://doi.org/10.17760/d20004844
Permanent Link:
http://hdl.handle.net/2047/d20004844
Use and reproduction:
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